Improved chemotherapy against breast cancer through immunotherapeutic activity of fucoidan decorated electrostatically assembled nanoparticles bearing doxorubicin

Pawar, Vivek K.; Singh, Yuvraj; Sharma, Komal; Shrivastav, Arpita; Sharma, Abhisheak; Singh, Atul Kumar; Meher, Jaya Gopal; Singh, Pankaj Kumar; Raval, Kavit; Kumar, Abdhesh; Bora, Himangshu K.; Datta, Dipak; Lal, Jawahar; Chourasia, Manish K.

doi:10.1016/j.ijbiomac.2018.09.059

Cited by 63 publications

(32 citation statements)

References 47 publications

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“…The results demonstrated that intravenously injected nanoparticles with DOX were characterized by stronger antiproliferative properties and higher tumor inhibitory activity than free DOX. The beneficial effects on the antitumor activity of the obtained nanoparticles were caused probably by immunomodulatory properties of fucoidan, which were confirmed in the in vitro studies [53]. In addition, fucoidan is characterized by the ability to improve the stability of metallic nanoparticles thereby contributing to the reduction of their toxicity.…”

Section: Fucoidan Application In the Pharmaceutical Technologymentioning

confidence: 74%

Possibilities of Fucoidan Utilization in the Development of Pharmaceutical Dosage Forms

2019

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Fucoidan is a polysaccharide built from L-fucose molecules. The main source of this polysaccharide is the extracellular matrix of brown seaweed (Phaeophyta), but it can be also isolated from invertebrates such as sea urchins (Echinoidea) and sea cucumbers (Holothuroidea). Interest in fucoidan is related to its broad biological activity, including possible antioxidant, anti-inflammatory, antifungal, antiviral or antithrombotic effects. The potential application of fucoidan in the pharmaceutical technology is also due to its ionic nature. The negative charge of the molecule results from the presence of sulfate residues in the C-2 and C-4 positions, occasionally in C-3, allowing the formation of complexes with other oppositely charged molecules. Fucoidan is non-toxic, biodegradable and biocompatible compound approved by Food and Drug Administration (FDA) as Generally Recognized As Safe (GRAS) category as food ingredient. Fucoidan plays an important role in the pharmaceutical technology, so in this work aspects concerning its pharmaceutical characteristics and designing of various dosage forms (nanoparticles, liposomes, microparticles, and semisolid formulations) with fucoidan itself and with its combinations with other polymers or components that give a positive charge were reviewed. Advantages and limitations of fucoidan utilization in the pharmaceutical technology were also discussed.

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Section: Fucoidan Application In the Pharmaceutical Technologymentioning

confidence: 74%

Possibilities of Fucoidan Utilization in the Development of Pharmaceutical Dosage Forms

2019

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“…Fucoidan is abundantly produced by marine algae and has many biological functions, including anti-tumor, anti-inflammatory, anti-oxidation effects, and the regulation of glucose metabolism. 7 – 11 In animal experiments, Beppu and Peng confirmed that the repeated oral administration of high purity fucoidan at a maximum dose of 1000 mg/kg within 30 days caused no abnormal changes to the liver, kidney, spleen, or gonad tissues. 12 , 13 At the same time, fucoidan was not associated with any genotoxic effects in mouse bone marrow cells.…”

Section: Introductionmentioning

confidence: 93%

“…20 As a significant clinical application, fucoidan has been found to improve the sensitivity of lung and breast cancer to chemotherapeutic drugs, which not only reduced the severity of related side effects, but also reduced the economic burden to patients. 10,11 In addition, many studies have reported that fucoidan also plays an important anti-cancer role in other tumors, including prostate cancer, gastric cancer, liver cancer, and large B cell lymphoma. 18,[21][22][23][24][25] However, the effects of fucoidan have not been verified in OC.…”

Section: Introductionmentioning

confidence: 99%

<p>The Natural Product Fucoidan Inhibits Proliferation and Induces Apoptosis of Human Ovarian Cancer Cells: Focus on the PI3K/Akt Signaling Pathway</p>

Liu

Yang

Peng

et al. 2020

CMAR

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Objective: Ovarian cancer (OC) is the leading cause of death among gynecological tumors; however, no effective treatment is currently available. Fucoidan, which is extracted from marine algae, has significant anti-cancer effects. The aim of this study was to determine the effects of fucoidan on the proliferation and apoptosis of OC cells through inhibition of the PI3K/Akt signaling pathway. Methods: Human ovarian normal epithelial cells (IOSE80) and human OC cells (SKOV-3, A2780, OVCAR-3, TOV-112D, and Caov-3) were selected to verify the safety of fucoidan at various doses in SKOV-3 and Caov-3 cells as well as a xenograft mouse model using various molecular biology techniques. Results: Fucoidan had no significant effect on normal ovarian epithelial cells, but had significantly inhibited the proliferation of OC cells, induced cell cycle arrest at the G0/G1 phase, increased the proportion of apoptotic cells and expression of pro-apoptotic proteins, and inhibited the expression of PI3K and phosphorylation of Akt, which could be partly rescued by IGF-1. Conclusion: Fucoidan had anti-tumor effects both in vivo and in vitro via a mechanism that is related to the inhibition of the PI3K/Akt signaling pathway.

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“…In [ 113 ] The authors have also observed that combination treatments enhanced intracellular ROS levels and reduced glutathione (GSH) levels in breast cancer cells, suggesting that induction of oxidative stress was an important event in the cell death induced by the combination treatments. Very recently, Pawar et al [ 114 ] prepared doxorubicin (DOX)-loaded nanoparticles (NPs) using fucoidan and evaluated them as an improved chemotherapy against breast cancer through the immunotherapeutic activity of fucoidan based on an in-vivo model using 4T1 induced tumor-bearing BALB/c mice. Structural characterization and antitumor effects of enzymatically digested fucoidans extracted from the brown alga Kjellmaniella crassifolia have been reported [ 115 ].…”

Section: Point-of-care Applications Of Bioactive Compoundsmentioning

confidence: 99%

State-of-the-Art Extraction Methodologies for Bioactive Compounds from Algal Biome to Meet Bio-Economy Challenges and Opportunities

et al. 2018

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Over the years, significant research efforts have been made to extract bioactive compounds by applying different methodologies for various applications. For instance, the use of bioactive compounds in several commercial sectors such as biomedical, pharmaceutical, cosmeceutical, nutraceutical and chemical industries, has promoted the need of the most suitable and standardized methods to extract these bioactive constituents in a sophisticated and cost-effective manner. In practice, several conventional extraction methods have numerous limitations, e.g., lower efficacy, high energy cost, low yield, etc., thus urges for new state-of-the-art extraction methodologies. Thus, the optimization along with the integration of efficient pretreatment strategies followed by traditional extraction and purification processes, have been the primary goal of current research and development studies. Among different sources, algal biome has been found as a promising and feasible source to extract a broader spectrum of bioactive compounds with point-of-care application potentialities. As evident from the literature, algal bio-products includes biofuels, lipids, polyunsaturated fatty acids, pigments, enzymes, polysaccharides, and proteins. The recovery of products from algal biomass is a matter of constant development and progress. This review covers recent advancements in the extraction methodologies such as enzyme-assisted extraction (EAE), supercritical-fluid extraction (SFE), microwave-assisted extraction (MAE) and pressurized-liquid extraction (PLF) along with their working mechanism for extracting bioactive compounds from algal-based sources to meet bio-economy challenges and opportunities. A particular focus has been given to design characteristics, performance evaluation, and point-of-care applications of different bioactive compounds of microalgae. The previous and recent studies on the anticancer, antibacterial, and antiviral potentialities of algal-based bioactive compounds have also been discussed with particular reference to the mechanism underlying the effects of these active constituents with the related pathways. Towards the end, the information is also given on the possible research gaps, future perspectives and concluding remarks.

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Improved chemotherapy against breast cancer through immunotherapeutic activity of fucoidan decorated electrostatically assembled nanoparticles bearing doxorubicin

Cited by 63 publications

References 47 publications

Possibilities of Fucoidan Utilization in the Development of Pharmaceutical Dosage Forms

Possibilities of Fucoidan Utilization in the Development of Pharmaceutical Dosage Forms

<p>The Natural Product Fucoidan Inhibits Proliferation and Induces Apoptosis of Human Ovarian Cancer Cells: Focus on the PI3K/Akt Signaling Pathway</p>

State-of-the-Art Extraction Methodologies for Bioactive Compounds from Algal Biome to Meet Bio-Economy Challenges and Opportunities

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