Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment

Abstract

Arcobacter -like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter -like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter- like spp. from marine and shellfish matrices . For this purpose, 14 different Arcobacter -like spp . mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD 420nm ) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences ( p < 0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter -like species growth was significantly higher in Arcobacter broth + 50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter -like spp .

A core of functional complementary bacteria infects oysters in Pacific Oyster Mortality Syndrome

“…Vibrio species associated with POMS were characterized by key virulence factors that are required to weaken oyster cellular defenses (Rubio et al, 2019; Piel et al, 2019). Members of the Arcobacter genus had also been associated with POMS-diseased oysters [41, 42], but the role of this genus in pathogenesis was not investigated more deeply due to limitations of culture-based techniques [43]. In the present study, we extended the core colonizing microbiota to five bacterial genera.…”

Microbial community structure in a host–parasite system: the case of Prussian carp and its parasitic crustaceans

“…2018; Rahman et al . 2020). The second dominant OTU, Corynebacterium , is often found in the environment and usually colonizes the blood, skin and mucous membranes of humans and animals (Baya et al .…”

The Prevalence of Arcobacteraceae in Aquatic Environments: A Systematic Review and Meta-Analysis

“…The use of molecular methods allows a faster and more sensitive detection of bacteria, being able to detect both viable and non-viable cells, as well as viable but not cultivable cells. Nonetheless, this methodology has some drawbacks as well, associated with the fact that some molecular methodologies do not allow to distinguish dead from live cells or to recover bacterial isolates that can be used for further studies [14,[23][24][25]. Several culture methods are used and the recovery of bacteria from this family can be associated with various factors related with the sample, but also with the disparity in the sensitivity and specificity of isolation methods [25], pointing out the need for a standard protocol for the isolation of Arcobacteraceae species from diverse samples [11,23,25].…”

“…Arcobacteraceae is a family found in diverse habitats, environments and hosts, including animals, humans, foods and food-processing facilities, environmental and water sources such as underground water, surface water, sewage and sea water [11][12][13]. In fact, most of the species from this family have been first isolated from aquatic environments [14]. Few species from this family have been associated with animal and human disease, among which Aliarcobacter butzleri and Aliarcobacter cryaerophilus have been classified by the International Commission on Microbiological Specification for Food as a serious hazard to human [15].…”