Improved derivative of 6-keto-prostaglandin F12 for gas chromatographic-mass spectrometric analysis

Bazan, A.; Knapp, Daniel R.

doi:10.1016/s0021-9673(00)82515-7

Cited by 35 publications

(1 citation statement)

References 12 publications

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“…208) with 2-(methoxycarbonyl)ethylsulphenyl chloride then furnished both the 6a-and the 6P-isomer, ( 209) and ( 210) respectively, after deprotection. Both (209) and (210) were inactive in the platelet-aggregation assay, although (209) showed some inhibition of ethanol-elicited gastric lesions. Szhntay et aL116 have reported syntheses of the 13-oxa-and 13-thia-prostacyclins ( 215) and (216).…”

Section: Thpomentioning

confidence: 96%

Prostaglandins, thromboxanes, leukotrienes, and related arachidonic acid metabolites

Lai¹,

Manley²

1984

Nat. Prod. Rep.

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Section: Thpomentioning

confidence: 96%

Prostaglandins, thromboxanes, leukotrienes, and related arachidonic acid metabolites

Lai¹,

Manley²

1984

Nat. Prod. Rep.

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Combined capillary column gas chromatography negative ion chemical ionization mass spectrometry of prostanoids

Waddell

Blair

Wellby

1983

Biol. Mass Spectrom.

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Electron capturing pentafluorobenzyl ester derivatives of prostanoids provide intense negative ion chemical ionization mass spectra. Fragmentation is directed almost entirely away from the prostanoid molecule and this provides intense [M-C,F,CH,]-ions. These ions are ideal for specific and sensitive quantitative selected ion monitoring analysis. The limits of sensitivity employing this technique are in the range 1-8 pg on column compared with 100-500 pg using the corresponding methyl ester derivative in the electron impact mode. The capillary column characteristics of the pentafluorobenzyl esters are suitable for the development of multiprostaglandin gas chromatographic mass spectrometric assays.

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Quantitative analysis of prostanoids in biological fluids by combined capillary column gas chromatography negative ion chemical ionization mass spectrometry

Waddell

Barrow

Robinson

et al. 1984

Biol. Mass Spectrom.

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An assay for the quantitative analysis of six biologically important prostanoids based on combined gas chromatography negative ion chemical ionization mass spectrometry has been developed. Prostanoids were extracted from biological fluids by liquid chromatography on Sep-Pak cartridges and converted to pentafluorobenzyl ester derivatives. Samples were injected on capillary column by the splitless technique and injections were made in a high boiling hydrocarbon solvent (n-dodecane) in order to minimize chromatographic run times. Quantification was carried out using selected ion monitoring of the appropriate [M-pentafluorobenzyl]- anion. The assay has been used for profiling cyclooxygenase metabolites of arachidonic acid in guinea pig lung perfusate after induction of anaphylaxis and platelet rich plasma after collagen-stimulated aggregation.

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Improved derivative of 6-keto-prostaglandin F12 for gas chromatographic-mass spectrometric analysis

Cited by 35 publications

References 12 publications

Prostaglandins, thromboxanes, leukotrienes, and related arachidonic acid metabolites

Prostaglandins, thromboxanes, leukotrienes, and related arachidonic acid metabolites

Combined capillary column gas chromatography negative ion chemical ionization mass spectrometry of prostanoids

Quantitative analysis of prostanoids in biological fluids by combined capillary column gas chromatography negative ion chemical ionization mass spectrometry

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