Improved efficiency of microspore culture of Brassica campestris ssp. pekinensis (Chinese cabbage)

Abstract

We selected two cultivars of Brassica campestris ssp. pekinensis (Chinese cabbage), 'Futian 50' and 'Changkuai,' to investigate the factors that influence microspore embryogenesis and plantlet regeneration. We have also discussed some protocols for the induction culture of microspore-derived embryos and for rooting and transplantation of microspore-derived plantlets. We obtained the following findings in our study. Although NAA promotes the development of embryo and reduces the percentage of abnormal embryos, it inhibits the formation of microspore-derived embryos. 2,4-D also inhibits the formation of microspore-derived embryos. Low concentrations of cytokinins facilitate embryogenesis, while high concentrations inhibit embryogenesis; BA has a stronger influence than zeatin (Z) on embryo induction. The combined effects of auxin and cytokinin are synergistic. Organic compounds increase the rate of formation of microspore-derived embryos. However, activated charcoal (AC) inhibits embryo development. The better the development of the embryos, the higher is the plantlet regeneration rate. The plant regeneration rate increased significantly on the MS culture medium supplemented with 200 mg·L -1 AC. The MS medium s suitable for the subculture of the regenerated plantlets. MS medium containing 0.1 mg·L -1 NAA is the optimal medium for rooting of microsporederived plantlets.

Mutagenic treatment of microspore‐derived embryos of turnip rape (Brassica rapa) to increase oleic acid content

“…Many researchers have also used culture media without plant growth regulators. However, Han, Ye, and Feng (2014) reported that BAP stimulates embryogenesis in B. campestris at a concentration of 0.4 mg/L. The results of our study did not fully correspond with the abovementioned works.…”

Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

“…A very similar response has been observed in Chinese cabbage (Brassica campestris spp. pekinensis) (Han et al, 2014), where the addition of AC increased plantlet regeneration. In several subspecies of Brassica rapa, further development of obtained microsporederived embryos can be approached in different ways (Takahashi, 2012).…”