Improved embryogenic callus induction and plant regeneration in big bluestem (Andropogon gerardii Vitman), a potential bioenergy feedstock

Abstract

The objective of this study was to develop an efficient regeneration protocol in big bluestem, a potential feedstock that produces huge biomass. ). Shoots were regenerated on all of the concentrations tested and the regeneration percentage and number of shoots per calli increased with the increase in BA or kinetin concentration. Regenerated shoots were transferred to half strength MS medium for rooting. The fully developed plantlets were established in the greenhouse. The regeneration protocol established in this study may be used for the application of genetic engineering technologies in big bluestem.