Improved endometrial assessment using cyclin E and p27

Dubowy, R.L; Feinberg, Ronald F.; Keefe, David L.; Doncel, Gustavo F.; Williams, Shaun C; McSweet, Juliette C.; Kliman, Harvey J.

doi:10.1016/s0015-0282(03)00573-9

Cited by 63 publications

(37 citation statements)

References 40 publications

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“…Although these criteria did not prove to be accurate or precise enough to diagnose luteal phase deficiency with validity, there is a continued search for new markers including ultrasonographic measurements, ultrastructural examination by electron microscopy, immunological markers, steroid hormones and receptors, growth factors, and other proteins and secreted factors [32]. Some of these biomarkers are integrated into specific tests, such as the E-tegrity [33] or the Endometrial Function Test [34]. More recently, with the development of the new B-omics^era, new methods continue to be developed.…”

Section: Discussionmentioning

confidence: 99%

Reproductive outcome is optimized by genomic embryo screening, vitrification, and subsequent transfer into a prepared synchronous endometrium

Rodriguez-Purata

Lee

Whitehouse

et al. 2016

J Assist Reprod Genet

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Purpose The aim of this study is to compare implantation and live birth rates (LBR) between fresh euploid embryo transfers versus cryo-all cycles with a subsequent embryo transfer into a prepared endometrium. Material and Methods This is a retrospective cohort study. Patients who underwent an IVF cycle with PGS with trophectoderm biopsy from January 2011 to July 2015 were included. Patients were divided into three groups: "Fresh Only," "Frozen Embryo Transfer ('FET) Only," and "Fresh ET then FET." For "Fresh Only" group (n = 345), PGS results were received within 24 h. For "FET Only" group (n = 514), results were expected after 24 h, and embryos were cryopreserved after biopsy; only FET was performed in this group (no fresh transfer). For "FET with a previous fresh ET" (n = 139) group, patients underwent a fresh ET with a subsequent FET, in which the same cohort of embryos was utilized. The main outcome measures were pregnancy rate (PR), clinical PR, implantation rate (IR), LBR, and early pregnancy loss rate. Results IRs were statistically higher in the "FET Only" group when compared to the "Fresh Only" group (59.5 vs. 50.6 %, p < 0.01) and the "FET with a previous fresh ET" (59.5 vs. 50.6 %, p < 0.05). LBR was statistically significant in the "FET Only" group when compared to the "Fresh Only" group (57.6 vs. 46.5 %, p < 0.005) but not when compared to "FET with a previous fresh ET" group (57.6 vs. 47.7 %, p = 0.07). Conclusions This analysis suggests euploid embryos to be more likely to implant and achieve a LBR in a synthetic FET cycle than in a fresh cycle.

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Section: Discussionmentioning

confidence: 99%

Reproductive outcome is optimized by genomic embryo screening, vitrification, and subsequent transfer into a prepared synchronous endometrium

Rodriguez-Purata

Lee

Whitehouse

et al. 2016

J Assist Reprod Genet

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“…The proliferating activity of stromal cell may depend on steroid hormones acting through cyclin E [22,23]. Some factors such as TGF-beta may regulate cell proliferation through P27, the cellc yc le i n hi b it o r , b ut n o ev i d en c e h as be en documented on the bovine endometrium [9,23].…”

Section: Discussionmentioning

confidence: 99%

Proliferative Potential of Endometrial Stromal Cells, and Endometrial and Placental Expression of Cyclin in the Bovine

Yamauchi

Takezawa

Kizaki

et al. 2003

Journal of Reproduction and Development

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Abstract. The objective of the present investigation was to study proliferative activity of fibroblast-like endometrial stromal cells in bovine endometrial caruncular (CAR) and intercaruncular (ICAR) areas that have distinct functions during implantation. Endometrial stromal cells were derived from CAR and ICAR of nonpregnant cows, and their proliferative potential was analyzed in an in vitro cell culture system. In addition, expression of four types of cell cycle regulatory molecules was analyzed by RT-PCR in samples of CAR, ICAR, cotyledon (COT) and fetal membrane of both artificially inseminated (AI) and somatic nuclear transferred (NT) cows on days 30 and 60 of gestation. The proliferation growth curve showed that the cells derived from CAR had higher proliferative activity than that of ICAR-derived cells. No morphological differences were found between the cells derived from CAR and ICAR at population-doubling levels (PDL) of the two. Most of the cells derived from ICAR of nonpregnant cows exhibited expanded shape with no further proliferation at PDL 6 with a lack of cyclin E expression. Of the regulatory molecules, cyclin D1, CDK2 and CDK4 were expressed in both CAR and ICAR cells derived from both nonpregnant, and AI cows on days 30 and 60 of gestation. The expression of cyclin E in both AI and NT cows was confined to COT and fetal membrane on day 30 of gestation. Cyclin E expression on day 60 of gestation in AI cows was observed in all but ICAR areas. In marked contrast, however, cyclin E expression on day 60 of gestation in NT cows was confined to COT, suggesting that poor placentation in these cows is possibly associated with a lack of cyclin E expression. These results suggest that CAR-derived stromal cells have higher proliferative potential, which may be related to cyclin E expression during implantation. Key words: Bovine, Endometrium, Caruncle, Cell culture, Cyclin (J. Reprod. Dev. 49: [553][554][555][556][557][558][559][560] 2003) emarkable structural and functional changes, which occur in the endometrium to prepare suitable environment for implantation, depend on many factors including steroid hormones and cytokines [1]. Although biochemical and molecular mechanisms responsible for these changes are still

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“…Many studies searching for endometrial markers have focused on single molecules or on specific families of molecules (Hynes 1992; Lessey et al, 1992;Meseguer et al, 2001;Dubowy et al, 2003) reaching the conclusion that a single molecule is not sufficient to describe such a complex phenomenon. By acknowledging this fact, transcriptomic profiles may prove to be complex enough to classify the different endometrial cycle phases, including the window of endometrial receptivity (Fig.…”

Section: Clinical Translation Of Endometrial Transcriptomics and Persmentioning

confidence: 99%

Transcriptomics of the human endometrium

Díaz-Gimeno

Ruiz-Alonso²,

Blesa³

et al. 2014

Int. J. Dev. Biol.

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During the mid-secretory phase, the endometrium acquires the receptive phenotype, which corresponds to the only period throughout the endometrial cycle in which embryo implantation is viable. Endometrial receptivity is a crucial process and even more important in Assisted Reproductive Technologies (ART) where embryo-endometrial synchronization is coordinated through embryo transfer timing. Over the last decade, transcriptomic analyses performed on the human endometrium have shown that specific genomic signatures can be used to successfully phenotype different phases of the menstrual cycle including the receptive stage, independently of the histological appereance of the endometrial tissue. In this paper, we review current evidence demonstrating that endometrial transcriptomics objectively identifies the implantation window in a personalized manner, opening the field for the diagnosis of the endometrial factor in ART and moving to stratified medicine at this level, using microarray technology and soon high-throughput next generation sequencing coupled with functional and systems genomics approach.

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Improved endometrial assessment using cyclin E and p27

Cited by 63 publications

References 40 publications

Reproductive outcome is optimized by genomic embryo screening, vitrification, and subsequent transfer into a prepared synchronous endometrium

Reproductive outcome is optimized by genomic embryo screening, vitrification, and subsequent transfer into a prepared synchronous endometrium

Proliferative Potential of Endometrial Stromal Cells, and Endometrial and Placental Expression of Cyclin in the Bovine

Transcriptomics of the human endometrium

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