Improved enzyme thermal stability, loading and bioavailability using alginate encapsulation

Weng, Yilun; Ranaweera, Supun; Zou, Da; Cameron, Anna P.; Chen, Xiaojing; Song, Hao; Zhao, Chun‐Xia

doi:10.1016/j.foodhyd.2022.108385

Cited by 19 publications

(3 citation statements)

References 51 publications

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“…There is an observed decrease at 190 nm and an increase at 220 nm when heat treatment destabilizes protein or secondary structures like β-sheet and α-helix ( Miles et al, 2021 ). Specifically, it has been reported that the α-helix content of proteins decreases when the temperature exceeds a protein-dependent specific range ( Weng et al, 2023 ). It has been demonstrated that α-helices play an important role in maintaining the structural conformation of proteins.…”

Section: Resultsmentioning

confidence: 99%

Improving the thermal stability of phytase using core-shell hydrogel beads

Yang,

Dong,

Khongkomolsakul

et al. 2024

Food Chemistry: X

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Section: Resultsmentioning

confidence: 99%

Improving the thermal stability of phytase using core-shell hydrogel beads

Yang,

Dong,

Khongkomolsakul

et al. 2024

Food Chemistry: X

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“…In addition, gastric acids triggered the transformation of the control NPs (88.3%) compared to the encapsulated curcumin (48%) and thus had significantly lower bioaccessible (9.1%) curcumin [173]. Similarly, in vitro simulated poultry GIT conditions demonstrated that encapsulation enhanced the release of enzymes from alginate NPs with concomitant good bioavailability of phosphorus and calcium in chicken [49]. A recent study showed that Phaeodactylum tricornutum (PE) extract encapsulated with alginate-casein (A-C-PE:PE) and chitosan (A-C-PE) showed a controlled release of fucoxanthin and improved adsorption (1.8-fold) using Caco-2/TC7 cells compared to control.…”

Section: Biosafety and Bioavailabilitymentioning

confidence: 98%

“…Natural polysaccharides, notably starch [48], cellulose [49], chitosan [50], inulin [51], dextrin [52], hyaluronic acid [53], fucoidan [54], and pectin [55], are used as substrates to synthesize amphiphilic polysaccharides via octenylsuccination [56], acetylation [57], and esterification with fatty acids [58]. In addition, semisynthetic polysaccharides with grafted hydrophobic groups and synthetic polysaccharides had attracted attention as wall materials [59].…”

Section: Carrier Classificationmentioning

confidence: 99%

Encapsulation of Hydrophobic Bioactive Substances for Food Applications: Carriers, Techniques, and Biosafety

Shavronskaya,

Noskova,

Skvortsova

et al. 2023

Journal of Food Processing and Preservation

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Biologically active substances (BASs) are used as novel ingredients to design functional foods. These functional foods are alternate approaches to treat or cure chronic diseases. However, the application of BASs is limited due to their hydrophobic nature, low bioavailability, sensitivity to gastric acid, and environmental conditions (i.e., high temperatures, radiation, pH, and oxygen). Thus, research has been channeled to find ways of curbing these limitations. This review provides an overview of the modern methods for BAS encapsulation, carrier classifications, benefits, and drawbacks as well as the biosafety of encapsulated BASs. Encapsulation of BASs into organic/inorganic carriers or composites overcomes the limitations mentioned above. In addition, encapsulation enables the controlled release of active compounds to target cells. The market for encapsulated foods has grown globally at a significant pace due to their various applications as functional foods, dietary supplements, and other products. It is estimated that by 2027, the market worth of encapsulated foods will be valued at $17 billion.

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Carbamoylase-based impedimetric electronic tongue for rapid detection of paralytic shellfish toxins

Raposo,

Soreto,

Moreirinha

et al. 2024

Anal Bioanal Chem

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Phytotoxins produced by marine microalgae, such as paralytic shellfish toxins (PSTs), can accumulate in bivalve molluscs, representing a human health concern due to the life-threatening symptoms they cause. To avoid the commercialization of contaminated bivalves, monitoring programs were established in the EU. The purpose of this work is the implementation of a PST transforming enzyme—carbamoylase—in an impedimetric test for rapid simultaneous detection of several carbamate and N-sulfocarbamoyl PSTs. Carbamoylase hydrolyses carbamate and sulfocarbamoyl toxins, which may account for up to 90% of bivalve toxicity related to PSTs. Conformational changes of carbamoylase accompanying enzymatic reactions were probed by Fourier transform mid-infrared spectroscopy (FT-MIR) and electrochemical impedance spectroscopy (EIS). Furthermore, a combination of EIS with a metal electrode and a carbamoylase-based assay was employed to harness changes in the enzyme conformation and adsorption on the electrode surface during the enzymatic reaction as an analytical signal. After optimization of the working conditions, the developed impedimetric e-tongue could quantify N-sulfocarbamoyl toxins with a detection limit of 0.1 µM. The developed e-tongue allows the detection of these toxins at concentration levels observed in bivalves with PST toxicity close to the regulatory limit. The quantification of a sum of N-sulfocarbamoyl PSTs in naturally contaminated mussel extracts using the developed impedimetric e-tongue has been demonstrated.

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Improved enzyme thermal stability, loading and bioavailability using alginate encapsulation

Cited by 19 publications

References 51 publications

Improving the thermal stability of phytase using core-shell hydrogel beads

Improving the thermal stability of phytase using core-shell hydrogel beads

Encapsulation of Hydrophobic Bioactive Substances for Food Applications: Carriers, Techniques, and Biosafety

Carbamoylase-based impedimetric electronic tongue for rapid detection of paralytic shellfish toxins

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