Carbon pool assessments in forests is one of the most important tasks of forest ecology. Despite the wide cultivation range, and economical and traditional importance, the aboveground biomass of European larch (Larix decidua Mill.) stands is poorly characterized. To increase knowledge about forest biomass accumulation and to provide a set of tools for aboveground biomass estimation, we studied a chronosequence of 12 larch forest stands (7-120 years old). From these stands, we measured the biomass of 96 sample trees ranging from 1.9 to 57.9 cm in diameter at breast height. We provided age-specific and generalized allometric equations, biomass conversion and expansion factors (BCEFs) and biomass models based on forest stand characteristics. Aboveground biomass of stands ranged from 4.46 (7-year-old forest stand) to 445.76 Mg ha −1 (106-year-old). Stand biomass increased with increasing stand age, basal area, mean diameter, height and total stem volume and decreased with increasing density. BCEFs of the aboveground biomass and stem were almost constant (mean BCEFs of 0.4688 and 0.3833 Mg m −3 , respectively). Our generalized models at the tree and stand level had lower bias in predicting the biomass of the forest stands studied, than other published models. The set of tools provided fills the gap in biomass estimation caused by the low number of studies on larch biomass, which allows for better estimation of forest carbon pools.Forest stand biomass can be estimated at two levels of detail: tree level and stand level. For tree level the most frequent approach is usage of allometric equations (AEs)-regression models allowing for tree biomass estimation using simple measurements, i.e., diameter at breast height or height [17][18][19]. AEs based on individual tree measurements require time-and money-consuming inventories, but yield higher accuracy of biomass estimation. Stand level estimation is usually conducted using forest stand models (e.g., [20,21]) or biomass expansion and conversion factors (BCEFs; e.g., [12,[22][23][24]). BCEFs are literally proportions of forest stand biomass and tree stand volume, allowing for easy conversion of forest inventory data (growing stock) into biomass. However, this approach is double-biased by errors of statistical models and errors of forest inventory. For example, BCEFs are not useful in cases of young forest stands where volume of merchantable wood is underestimated [21].The need for elaboration of more accurate biomass models is connected with the variability of biomass allocation patterns. The proportions of carbohydrates invested in development of particular organs vary climatically [25], but also with increasing age (e.g., [26][27][28]). For that reason, younger trees need separate models both at the tree (e.g., [23,29,30]) and stand (e.g., [21,22,24]) levels. Moreover, according to metabolic scaling theory [31] and its expansion [32], biomass allocation is connected with tree dimensions. In cases of managed forests, initial spacing of forest stands also influence both bioma...