Improved evaluation of the genetic variability of Brazilian strains of Erwinia psidii with newly developed microsatellite markers

Abstract

Erwinia psidii is the causal agent of bacterial blight of guava, and wilt and dieback of eucalypt in Brazil. Previous studies using repetitive sequence‐based PCR (rep‐PCR) revealed limited genetic diversity in E. psidii populations. Here, the draft genome sequence of the type strain IBSBF435 was probed for variable number of tandem repeats (VNTRs) that could be used to study E. psidii genetic diversity by multilocus variable number of tandem repeat analysis (MLVA). Eleven selected VNTRs were used to assess genetic relatedness among 108 E. psidii strains from guava (36) and eucalypt (72), of different geographic origins and years of collection. A total of 79 haplotypes were detected in the strain collection. The number of alleles per locus ranged from 3 to 15. A minimum spanning tree indicated the occurrence of nine clonal complexes, of which two contained only guava strains, five only eucalypt strains, and two contained guava and eucalypt strains. The three oldest strains collected from guava in the 1980s (IBSBF435, IBSBF454, and IBSBF493) exhibited distinct haplotypes. IBSBF435 was genetically more closely related to some eucalypt strains than to guava strains, which supports previously reported lack of host specificity. IBSBF454 grouped with guava strains more recently collected from different states, suggesting that it could have been disseminated to different Brazilian areas. The implication of the lack of differentiation between guava and eucalypt strains is discussed in relation to the detection of E. psidii affecting eucalypt more than 25 years after the first description of guava bacterial blight.

Phylloplane as a potential Erwinia psidii inoculum reservoir

“…The biological aspects of E. psidii have been extensively studied (Teixeira et al, 2009;, which has made it possible to establish standards for characterizing the genetic variability of the pathogen (Teixeira et al, 2009;Hermenegildo et al, 2021) and determine some components involved in virulence (Montoya-Estrada et al, 2019a; Pereira et al, 2021). However, studies on the bacterium's lifestyle are poor, which limits the understanding of the bacterial pathogen's life cycle in association with the host or under planted forest conditions.…”

Genetic diversity of eight maize (Zea mays) cultivars from East Nusa Tenggara (Indonesia) based on inter simple sequence repeat markers

“…The detection methods of genetic diversity using molecular markers are commonly categorized into three groups: DNA sequence-based (Nikmah et al 2016), hybridization-based, andPCR-based (Wahyudi et al 2020b). Several DNA molecular methods are frequently used to understand genetic characteristics (Govindaraj et al 2015), such as Restriction Fragment Length Polymorphism (RFLP) (Hapsari et al 2015), Random Amplified Polymorphic DNA (RAPD) (Uslan and Pharmawati 2020), Variable Number Tandem Repeats (VNTR) (Hermenegildo et al 2021), and Sequence-Related Amplified Polymorphism (Wahyudi et al 2020b). One of the approaches, Inter Simple Sequence Repeat (ISSR), is thought to be more robust than others and generates more polymorphism bands than RAPD (Das et al 2018;Poerba et al 2018).…”

Sobrevivência e herança da resistência a Erwinia psidii em eucalipto

“…Parte das sementes coletadas foi enviada à Clonar Resistência a Doenças Florestais LTDA (Cajuri, Minas Gerais, Brasil) para produção de mudas e inoculações em condições controladas.(2014). Desde então, este tem sido o isolado mais utilizado em pesquisas do patossistema Erwinia psidii × Eucalyptus spp Montoya-Estrada et al 2019Hermenegildo et al 2021;. Prereira et al 2021).…”