Background:We assessed the current status of knowledge, perception, attitude, and role model regarding hand hygiene (HH) among infection control nurses (ICNs) and identified the factors influencing these variables. Methods: A structured questionnaire was adapted from a World Health Organization survey. Data were collected from Results: ICNs showed the following scores (mean § SD): knowledge (19.5 § 2.3), perception (69.9 § 8.9), attitude (46.9 § 5.8), and role model (39.2 § 6.0). HH performance of health care workers (HCWs) was 75.2 § 15.5. Mean HH performance scores of HCWs (P = .007) differed significantly according to infection control experience (3 groups: ≤ 12 months, 13-24 months, > 24 months). Perception, attitude, and role model scores showed positive correlations with each other (P < .01). The regression model for HH performance of HCWs was calculated as follows: Y 1 = 31.638 + 0.067X 1 (perception of ICNs) + 0.133X 2 (attitude of ICNs) + 0.825X 3 (role model of ICNs) (P < .001; adjusted R 2 = 0.115). Discussion: Perception, attitude, and role model scores of ICNs were significant predictors of HH performance of HCWs.Conclusions: Specialized well-structured HH education programs should be developed for ICNs that will help improve HH performance of HCWs.