Improved hatching in mouse embryos brought about by combined partial zona dissection and co-culture

Khalifa, Essam-Aldein M.; Tucker, Michael; Hunt, Patricia P.; Hamidi, Jamal

doi:10.1093/oxfordjournals.humrep.a138103

Cited by 14 publications

(5 citation statements)

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“…However, historical comparison of two studies performed by the same group, where co-culture alone (Wiemer et al,, 1993) and AH + CC were performed, strongly suggests an additive beneficial effect of the two techniques combined. This agrees with the all-way comparison of standard culture, co-culture alone, AH-alone, and AH+CC in the mouse (Khalifa et al, 1993), where the combination AH + CC group outperformed all the other groups in terms of complete blastocyst hatching.…”

Section: Discussionsupporting

confidence: 88%

“…Cohen et al (1990) made the initial observation that a combination of assisted hatching and co-culture was apparently incompatible. However, subsequent study in the mouse (Khalifa et al, 1993) enabled a comparison of rates of blastocyst hatching relative to the use of assisted hatching and/or co-culture to be made, and showed that blastocyst hatching was optimally and additively enhanced by combination of both techniques. Also, combination of 2 days of co-culture and day 3 assisted hatching in human IVF has recently been shown not only to be feasible but also suggestive of being significantly advantageous to certain poor prognosis patients .…”

Section: Introductionmentioning

confidence: 99%

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Assisted hatching with or without bovine oviductal epithelial cell co-culture for poor prognosis in-vitro fertilization patients

et al. 1994

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Older patients and those who consistently return for embryo transfer but without implantation were studied to see if a combination of day 3 assisted hatching and co-culture (AH+CC) might be beneficial compared to assisted hatching alone (AH-alone). Female patients of > or = 38 years and couples who had previously failed to implant embryos three times or more were prospectively and randomly assigned to either an experimental or a control group. In the experimental group all embryos were co-cultured on partial monolayers of bovine oviductal epithelial cells for 2 days followed by assisted hatching by zona drilling (AH+CC). All control embryos were cultured by standard procedures until day 3 when they also underwent zona drilling prior to uterine transfer (AH-alone). With 50 cycles in each group there was unfortunately a marginal bias against the AH+CC group in that these patients had undergone a higher number of previous transfer cycles. There was a marginally lower percentage of fragmentation and a significantly higher degree of zona thickness variability in the AH+CC embryo group. Embryonic implantation was significantly increased (P < 0.05) in the AH+CC group (18%) when compared to the AH-alone group (10%). This difference was reflected in a significantly higher (P < 0.05) initial pregnancy rate (52 versus 32%) in the AH+CC group, and a higher (not significant) viable pregnancy rate (38 versus 22%).

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Assisted hatching with or without bovine oviductal epithelial cell co-culture for poor prognosis in-vitro fertilization patients

et al. 1994

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“…Next, matured oocytes were inseminated with heparin-treated (20 IU/ml) frozen-thawed bull spermatozoa. After 20 to 22 h zygotes were placed in co-culture with the bovine oviduct epithelial cell monolayer in Menezo B2 medium 4 (Khalifa et al, 1993) for 6 to 8 days to develop up to the blastocyst stage. Embryos at middle, late or expanded blastocyst stages were used.…”

Section: In Vitromentioning

confidence: 99%

“…By scraping the needle along the holding pipette the external surface of the zona pellucida was broken along the area where the needle was inserted. This procedure of the partial zona disection was based, in part, on the method used by Malter and Cohen (1989) and Khalifa et al (1993).…”

Section: Perforating the Zona Pellucidamentioning

confidence: 99%

Cattle twins after transfer of demi-embryos derived from zona-perforated blastocysts

Skrzyszowska¹,

Smorag²,

Ka̧tska³

et al. 1999

J. Anim. Feed Sci.

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This experiment investigated a method of bovine embryo bisection during hatching stimulated by a zona pellucida perforation. The method of bisection was intended to significantly lower cell loss which occurs during standard bisection. Blastocysts, obtained either in vitro or in vivo, had their zona pellucida perforated by puncturing or slitting near the inner cell mass and subsequently were cultured in vitro. During culture, hatching was characterized by the extrusion of the inner cell mass as well as trophoectodermal cells through the opening in the zona pellucida. At a certain point the hatching the blastocyst took on a figure eight shape. Cutting the narrow bridge of cells which connected both parts of the embryo led to a splitting of the embryo into two equivalent parts.The experiment determined the factors responsible for effective hatching stimulated by earlier zona pellucida perforation. The optimal stage of bovine embryo development for obtaining the highest proportion of eight-in-shape hatching was late blastocyst. At this stage eight-in-shape hatching occurred in 50.4% of the embryos (137/272) while in the case of middle blastocyst 28.3% (17/60) and expanding blastocysts 15.3% (11/72). In the cases of puncturing and slitting the zona pellucida there was a similar proportion of eight-in-shape hatching, 137/272 (50.4%) and 93/190 (48.9%), respectively.Demi-embryos produced from thirty-five blastocysts bisected by the eight-in-shape method (modified bisection) were transferred into 41 recipients. Seventeen recipients became pregnant. All 17 calved, resulting in 20 calves, including 4 sets of monozygotic twins.In the control group there were 13 recipients which received halves obtained from conventional blastocyst bisection. Two recipients became pregnant, resulting in 3 calves, including 1 set of monozygotic twins.It can be concluded that the developed procedure of splitting embryos during stimulated hatching may be an alternative to standard embryo bisection.

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“…Zygotes produced after subzonal injection of human spermatozoa showed a greater potential for development to cleaved embryos and pregnancy when cocultured with Vero cells (12). Utilization of coculture after partial zona dissection of mouse embryos has resulted in greater numbers of hatched blastocysts (13). In a similar study embryos from couples with three or more failed attempts at IVF were treated with coculture on bovine oviduct epithelial cells (BOEC) followed by assisted hatching.…”

Section: Introductionmentioning

confidence: 99%

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Nematollahi

Valojerdi

1999

Journal of Assisted Reproduction and Genetics

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Improved hatching in mouse embryos brought about by combined partial zona dissection and co-culture

Cited by 14 publications

References 0 publications

Assisted hatching with or without bovine oviductal epithelial cell co-culture for poor prognosis in-vitro fertilization patients

Assisted hatching with or without bovine oviductal epithelial cell co-culture for poor prognosis in-vitro fertilization patients

Cattle twins after transfer of demi-embryos derived from zona-perforated blastocysts

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