Improved Hepatic Regeneration With Reduced Injury by Redox Factor-1 in a Rat Small Size-Liver Transplant Model

Zinc Finger Protein A20 Promotes Regeneration of Small-for-Size Liver Allograft and Suppresses Rejection and Results in a Longer Survival in Recipient Rats

“…For small-for-size liver transplant, the median and left lateral lobes of the graft were resected following the standard donor operation to obtain 30% small-sized liver graft [6,19]. Ex vivo gene transfer in liver graft were performed as previously described [20]. The liver grafts were washed with ice-cold physiological saline (PS), and immediately infused with PS (PS rats), 5 ϫ 10 9 pfu rAdEasy-A20 (A20 rats) or 5 ϫ10 9 pfu rAdEasy (rAdEasy rats) through the portal vein (total volume, 0.6 mL).…”

Ex vivo adenoviral gene transfer of constitutively activated STAT3 reduces post-transplant liver injury and promotes regeneration in a 20% rat partial liver transplant model

“…Rat partial hepatectomy and ex vivo gene transfer was performed as previously described [26, 27]. Briefly, adult male Lewis rats weighing 230–250 g were used as donors and recipients.…”

“…Orthotopic liver transplantation of the partial liver grafts was performed with revascularization but without hepatic artery reconstruction, as previously described [26, 27]. In brief, the donor liver was flushed via the portal vein with ice‐cold PS, and the suprahepatic vena cava was anastomosed by 7–0 continuous sutures.…”

Comparative proteome profile during the early period of small-for-size liver transplantation in rats revealed the protective role of Prdx5

“…Clinical data [5] shows that survival in adult recipients undergoing LDLT is significantly lower than that in children. Size mismatch of graft and recipient remains a major risk factor contributing to impaired postoperative graft function [5,6], and the degree of graft damage is inversely related to graft size in SFSLT [7][8][9]. IRI occurs in a biphasic manner [10] in liver transplantation.…”