Improved human islets’ viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro

Camille, Laporte; Tubbs, Emily; Pierron, Maxime; Gallego, Amanda; Moisan, Anaïck; Lamarche, Frédéric; Lozano, Tamara; Hernández, Andrea; Cottet‐Rousselle, Cécile; Gauchez, Anne-Sophie; Persoons, Virginie; Bottausci, Frédéric; Fontelaye, Caroline; Boizot, François; Lablanche, Sandrine; Rivera, Florence

doi:10.1016/j.bbrc.2020.05.107

Cited by 25 publications

(17 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microcapsules (ø 500 µm) were produced using an in‐house microfluidic device as described elsewhere 17 . Capsules shared shape, size and quality parameters.…”

Section: Methodsmentioning

confidence: 99%

Semi‐automated digital quantification of cellular infiltrates for in vivo evaluation of transplanted islets of Langerhans encapsulated with bioactive materials

Ramirez

Courtoy

Kharrat

et al. 2021

Xenotransplantation

View full text Add to dashboard Cite

Background In the field of xenotransplantation, digital image analysis (DIA) is an asset to quantify heterogeneous cell infiltrates around transplanted encapsulated islets. Materials and Methods RGD‐alginate was used to produce empty capsules or to encapsulate neonatal porcine islets (NPI) with different combinations of human pancreatic extracellular matrix (hpECM), porcine mesenchymal stem cells (pMSC) and a chitosan anti‐fouling coating. Capsules were transplanted subcutaneously in rats for one month and then processed for immunohistochemistry. Immunostainings for macrophages (CD68) and lymphocytes (CD3) were quantified by DIA in two concentric regions of interest (ROI) around the capsules. DIA replicability and reproducibility were assessed by two blind operators. Repeatability was evaluated by processing the same biopsies at different time points. DIA was also compared with quantification by point counting (PC). Results Methodology validation: different sizes of ROIs were highly correlated. Intraclass correlation coefficients confirmed replicability and reproducibility. Repeatability showed a very strong correlation with CD3 stains and moderate/strong for CD68 stains. Group comparisons for CD68 IHC at each time point proved internal consistency. Point counting and DIA were strongly correlated with both CD3 and CD68. Capsule biocompatibility: Macrophage infiltration was higher around capsules containing biomaterials than around empty and RGD‐alginate‐NPI capsules. Lymphocytic infiltration was comparable among groups containing cells and higher than in empty capsules. Conclusion We validated a semi‐automated quantification methodology to assess cellular infiltrates and successfully applied it to investigate graft biocompatibility, showing that neonatal porcine islets encapsulated in alginate alone triggered less infiltration than capsules containing islets and bioactive materials.

show abstract

“…Microcapsules (ø 500 µm) were produced using an in‐house microfluidic device as described elsewhere 17 . Capsules shared shape, size and quality parameters.…”

Section: Methodsmentioning

confidence: 99%

Semi‐automated digital quantification of cellular infiltrates for in vivo evaluation of transplanted islets of Langerhans encapsulated with bioactive materials

Ramirez

Courtoy

Kharrat

et al. 2021

Xenotransplantation

View full text Add to dashboard Cite

show abstract

“…Based on these evidences, Laporte et al. developed a biocompatible composite capsule by combining M-rich alginate, RGD G-rich alginate and MSCs ( 139 ). This capsule composition improved the deleterious effect of encapsulation on human islets in vitro , showing decreased caspase activity and increased VEGF secretion.…”

Section: Mscs and Islet Co-transplantation For Islet Function Protectionmentioning

confidence: 99%

Engineering Islets From Stem Cells: The Optimal Solution for the Treatment of Diabetes?

Zhang

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Diabetes is a metabolic disease characterized by insulin deficiency. Bioengineering of stem cells with the aim to restore insulin production and glucose regulation has the potential to cure diabetic patients. In this review, we focus on the recent developments for bioengineering of induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and pancreatic progenitor cells in view of generating insulin producing and glucose regulating cells for β-cell replacement therapies. Recent clinical trials using islet cells derived from stem cells have been initiated for the transplantation into diabetic patients, with crucial bottlenecks of tumorigenesis, post-transplant survival, genetic instability, and immunogenicity that should be further optimized. As a new approach given high expectations, bioengineered islets from stem cells occupies considerable potential for the future clinical application and addressing the treatment dilemma of diabetes.

show abstract

“…On the other hand, insulin secretion was significantly higher in MSCs and RGD-containing encapsulated cells than in the two other samples. Also, results showed that insulin secretion increases 2.5% in those microcapsules containing only β -cells and 2.9% in microcapsules, which also included MSCs and RGD ( Laporte et al, 2020 ). This study concluded that RGD incorporation in the alginate microcapsules and co-culturing with MSCs could remarkably improve cell viability and functionality.…”

Section: Hydrogel-based Encapsulation Devicesmentioning

confidence: 99%

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Ghasemi

Akbari

Imani

2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Islet transplantation provides a promising strategy in treating type 1 diabetes as an autoimmune disease, in which damaged β-cells are replaced with new islets in a minimally invasive procedure. Although islet transplantation avoids the complications associated with whole pancreas transplantations, its clinical applications maintain significant drawbacks, including long-term immunosuppression, a lack of compatible donors, and blood-mediated inflammatory responses. Biomaterial-assisted islet transplantation is an emerging technology that embeds desired cells into biomaterials, which are then directly transplanted into the patient, overcoming the aforementioned challenges. Among various biomaterials, hydrogels are the preferred biomaterial of choice in these transplants due to their ECM-like structure and tunable properties. This review aims to present a comprehensive overview of hydrogel-based biomaterials that are engineered for encapsulation of insulin-secreting cells, focusing on new hydrogel design and modification strategies to improve β-cell viability, decrease inflammatory responses, and enhance insulin secretion. We will discuss the current status of clinical studies using therapeutic bioengineering hydrogels in insulin release and prospective approaches.

show abstract

Improved human islets’ viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro

Cited by 25 publications

References 21 publications

Semi‐automated digital quantification of cellular infiltrates for in vivo evaluation of transplanted islets of Langerhans encapsulated with bioactive materials

Semi‐automated digital quantification of cellular infiltrates for in vivo evaluation of transplanted islets of Langerhans encapsulated with bioactive materials

Engineering Islets From Stem Cells: The Optimal Solution for the Treatment of Diabetes?

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Contact Info

Product

Resources

About