Improved identification of Streptococcus bovis-Streptococcus equinus-complex species and subspecies by MALDI-TOF MS using a novel library

Öberg, Jonas; Inghammar, Malin; Nilson, Bo

doi:10.1016/j.diagmicrobio.2023.116045

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Matrix-Assisted Laser Desorption/Ionization Time-of Flight Mass Spectrometry (MALDI-TOF MS) has been widely applied for the fast and accurate clinical identification of microbial species in recent years. MALDI-TOF MS could identify different subspecies ( Wang et al, 2022 ; Liu et al, 2023 ; Oberg et al, 2023 ), like automated, fast and accurate prediction of methicillin-resistant Staphylococcus aureus (MRSA) clonal complexes (CCs) ( Camoez et al, 2016 ) and GBS subspecies-level typing based on the mass variation of ribosomal subunit proteins (rsp profile) ( Rothen et al, 2019 ). The previous reported GBS subtyping strategies based on MALDI-TOF MS all require manual assessment of the acquired spectra and highly trained personnel with professional tools like ClinProTool software or online GBS serotyper, including peak biomarkers of different GBS sequence types (STs) and serotypes ( Lartigue et al, 2011 ; Lanotte et al, 2013 ; Lin et al, 2019 ), the statistical models generated based on MALDI-TOF MS spectrometry for the rapid classification of major GBS serotypes (Ia, Ib, III, V, VI) ( Wang et al, 2019 ) and STs (ST10, ST12, ST17, ST19) ( Huang et al, 2020 ), or the facile machine learning GBS CCs multi-classification model generated through XGBoost algorithm based on the antibiotic susceptibility, serotypes and virulence genes of GBS strains ( Liu et al, 2022 ), making them not suitable for routine clinical application.…”

Section: Introductionmentioning

confidence: 99%

Automated classification of group B Streptococcus into different clonal complexes using MALDI-TOF mass spectrometry

Huang,

Gao,

Zhong

et al. 2024

Front. Mol. Biosci.

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ObjectivesTo evaluate the performance of Matrix-Assisted Laser Desorption/Ionization Time-of Flight Mass Spectra (MALDI-TOF MS) for automated classification of GBS (Group B Streptococcus) into five major CCs (clonal complexes) during routine GBS identification.MethodsMALDI-TOF MS of 167 GBS strains belonging to five major CCs (CC10, CC12, CC17, CC19, CC23) were grouped into a reference set (n = 67) and a validation set (n = 100) for the creation and evaluation with GBS CCs subtyping main spectrum (MSP) and MSP-M using MALDI BioTyper and ClinProTools. GBS CCs subtyping MSPs-M was generated by resetting the discriminative peaks of GBS CCs subtyping MSP according to the informative peaks from the optimal classification model of five major CCs and the contribution of each peak to the model created by ClinProTools.ResultsThe PPV for the GBS CCs subtyping MSP-M was greater than the subtyping MSP for CC10 (99.21% vs. 93.65%), but similar for CC12 (79.55% vs. 81.06%), CC17 (93.55% vs. 94.09%), and CC19 (92.59% vs. 95.37%), and lower for CC23 (66.67% vs. 83.33%).ConclusionMALDI-TOF MS could be a promising tool for the automated categorization of GBS into 5 CCs by both CCs subtyping MSP and MSP-M, GBS CCs subtyping MSP-M is preferred for the accurate prediction of CCs with highly discriminative peaks.

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Section: Introductionmentioning

confidence: 99%

Automated classification of group B Streptococcus into different clonal complexes using MALDI-TOF mass spectrometry

Huang,

Gao,

Zhong

et al. 2024

Front. Mol. Biosci.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid analysis of eight Gelsemium elegans Benth alkaloids in human plasma and urine

Zeng,

Wang,

Luo

et al. 2024

One Health Adv.

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Gelsemium elegans Benth alkaloids are the main components of G. elegans and can cause acute toxicosis or even death. Although several studies have reported methods for detecting G. elegans alkaloids, a high-throughput and environmental-friendly strategy for detection of multiple G. elegans alkaloids has not been realized. In this work, a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method was developed for rapid detection of G. elegans alkaloids in human plasma and urine for diagnosis of poisoning. Multiple matrices and crystal spotting methods were evaluated to obtain stable and high peak intensities without “sweet spot”. We verified the methodology and obtained excellent results. The matrix effects with different dilutions were compared and good recoveries and a low relative standard deviation were obtained with a 40-fold dilution. This method could shorten the analysis time and greatly reduce the consumption of chemical solvents. Furthermore, it could be applied to quantitative assessment of G. elegans alkaloid poisoning incidents.

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Improved identification of Streptococcus bovis-Streptococcus equinus-complex species and subspecies by MALDI-TOF MS using a novel library

Cited by 2 publications

References 13 publications

Automated classification of group B Streptococcus into different clonal complexes using MALDI-TOF mass spectrometry

Automated classification of group B Streptococcus into different clonal complexes using MALDI-TOF mass spectrometry

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid analysis of eight Gelsemium elegans Benth alkaloids in human plasma and urine

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