Improved initial osteoblast functions on amino-functionalized titanium surfaces

Nebe, Barbara; Finke, Birgit; Lüthen, Frank; Bergemann, Claudia; Schröder, Karsten; Rychly, Joachim; Liefeith, Klaus; Ohl, A.

doi:10.1016/j.bioeng.2007.07.004

Cited by 88 publications

(83 citation statements)

References 25 publications

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“…But actually, the matrix substance hyaluronan [8,9] seems to take a key role in initial interface reactions. Thus, a surface functionalization process was developed based on a positively charged implant surface to interact with the negative charge of hyaluronan which is due to the carboxyl group of the glucuronic acid [10,11]. An amino groups carrying surface would present the desired properties, because these groups are protonated in aqueous solutions at physiological pH values and therefore generate positive charges.…”

Section: Introductionmentioning

confidence: 99%

Gas-Discharge Plasma-Assisted Functionalization of Titanium Implant Surfaces

Schröder

Finke

Polák

et al. 2010

MSF

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Abstract.A crucial factor for in-growth of metallic implants in the bone stock is the rapid cellular acceptance whilst prevention of bacterial adhesion on the surface. Such contradictorily adhesion events could be triggered by surface properties. There already exists fundamental knowledge about the influence of physicochemical surface properties like roughness, titanium dioxide modifications, cleanness, and (mainly ceramic) coatings on cell and microbial behavior in vitro and in vivo.The titanium surface can be equipped with antimicrobial properties by plasma-based copper implantation, which allows the release and generation of small concentrations of copper ions during contact with water-based biological liquids.Additionally, the titanium surface was equipped with amino groups by the deposition of an ultrathin plasma polymer. This coating on the one hand does not significantly reduce the generation of copper ions, and on the other hand improves the adhesion and spreading of osteoblast cells.The process development was accompanied by physicochemical surface analyses like XPS, FTIR, contact angle, SEM, and AFM. Very thin modified layers were created, which are resistant to hydrolysis and delamination. These titanium surface functionalizations were found to have either an antimicrobial activity or cell-adhesive properties. Intramuscular implantation of titanium samples coated with the cell-adhesive plasma polymer in rats revealed a reduced inflammation reaction compared to uncoated titanium.

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Section: Introductionmentioning

confidence: 99%

Gas-Discharge Plasma-Assisted Functionalization of Titanium Implant Surfaces

Schröder

Finke

Polák

et al. 2010

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“…At pH 7.4, ALN results in positive zeta potentials, while RIS results in negative ones as a function of concentration, most likely due to the differing nitrogen charges/species among the two bisphosphonates. (1) Cells may sense and react to this potential, (46)(47)(48)(49)(50)(51)(52)(53) thus it is plausible that the different affinities and zeta-potentials influence osteoblastic function differently, quantitatively and/or qualitatively.…”

Section: Discussionmentioning

confidence: 99%

Effects of alendronate and risedronate on bone material properties in actively forming trabecular bone surfaces

Hofstetter

Gamsjaeger

Phipps³

et al. 2012

Journal of Bone and Mineral Research

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We used Raman and Fourier transform infrared microspectroscopy (FTIRM) analysis to examine the intrinsic bone material properties at actively bone-forming trabecular surfaces in iliac crest biopsies from women with postmenopausal osteoporosis (PMO) who were treated with either alendronate (ALN) or risedronate (RIS). At eight study sites, women were identified who had postmenopausal osteoporosis (PMO), were at least 5 years postmenopause, and had been on long-term therapy (either 3-5 years or >5 years) with daily or weekly ALN or RIS. Following standard tetracycline labeling, biopsies were collected from 102 women (33 treated with ALN for 3-5 years , 35 with ALN for >5 years , 26 with RIS for 3-5 years , and 8 with RIS for >5 years ) and were analyzed at anatomical areas of similar tissue age in bone-forming areas (within the fluorescent double labels). The following outcomes were monitored and reported: mineral to matrix ratio (corresponding to ash weight), relative proteoglycan content (regulating mineralization commencement), mineral maturity (indicative of the mineral crystallite chemistry and stoichiometry, and having a direct bearing on crystallite shape and size), and the ratio of two of the major enzymatic collagen cross-links (pyridinoline/divalent). In RIS-5 there was a significant decrease in the relative proteoglycan content (À5.83% compared to ALN-5), while in both RIS-3 and RIS-5 there was significantly lower mineral maturity/ crystallinity (À6.78% and À13.68% versus ALN-3 and ALN-5, respectively), and pyridinoline/divalent collagen cross-link ratio (À23.09% and À41.85% versus ALN-3 and ALN-5, respectively). The results of the present study indicate that ALN and RIS exert differential effects on the intrinsic bone material properties at actively bone-forming trabecular surfaces. ß

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“…Comparison of the initial osteoblast adhesion on titanium biofunctionalized with PPAAm vs. a COL-treated surface. Note that already after 2 min cell adhesion is impressively improved on PPAAm which is significant, but not for cell adhesion on COL (details see Nebe et al, 2007). Human MG-63 cells, adherent cells related to Ti_P=1, flow cytometric approach, T-test paired, *p<0.01. experiments can be found elsewhere Nebe et al, 2007;Rebl et al, 2010a).…”

Section: Improvement Of Osteoblast Adhesion: Comparision Of a Plasma mentioning

confidence: 99%

“…Note that already after 2 min cell adhesion is impressively improved on PPAAm which is significant, but not for cell adhesion on COL (details see Nebe et al, 2007). Human MG-63 cells, adherent cells related to Ti_P=1, flow cytometric approach, T-test paired, *p<0.01. experiments can be found elsewhere Nebe et al, 2007;Rebl et al, 2010a). Furthermore, covalent coupling reactions of the plasma generated amino groups with collagen I (COL) were carried out to compare the cell adhesion between solely surface charges received by PPAAm and additionally immobilized COL. For the immobilization of collagen I the bifunctional spacer polyethylene glycol diacid (PEG DA) catalyzed by carbodiimide, was used.…”

Section: Improvement Of Osteoblast Adhesion: Comparision Of a Plasma mentioning

confidence: 99%

“…Due to plasma processes the number of hydroxyl groups on biomaterial surfaces can be increased and reactive functional amino-or carboxyl groups can be deposited (Schröder et al, , 2010c. Plasma polymerized allylamine (PPAAm) coatings induce strong cell-adhesive effects (Nebe et al, 2010;Nebe et al, 2007;Finke et al, 2007). Decisive for plasma applications on biomaterial surfaces is if cell physiology can be stimulated by the deposited functional groups of its own or, if additional immobilization of biorelevant molecules is necessary.…”

Section: Introductionmentioning

confidence: 99%

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Improved initial osteoblast functions on amino-functionalized titanium surfaces

Cited by 88 publications

References 25 publications

Gas-Discharge Plasma-Assisted Functionalization of Titanium Implant Surfaces

Gas-Discharge Plasma-Assisted Functionalization of Titanium Implant Surfaces

Effects of alendronate and risedronate on bone material properties in actively forming trabecular bone surfaces

On the Application of Gas Discharge Plasmas for the Immobilization of Bioactive Molecules for Biomedical and Bioengineering Applications

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