Improved MALDI-TOF MS Identification of Mycobacterium tuberculosis by Use of an Enhanced Cell Disruption Protocol

Bacanelli, Gisele; Araújo, Flábio R.; Verbisck, Newton V.

doi:10.3390/microorganisms11071692

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…Each ATCC isolate was stored as a bacterial suspension in tryptone soy broth containing 30% glycerol at −80 °C. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method used to identify bacterial isolates and was used in this study to determine the identity and integrity of all bacterial strains prior to assaying.…”

Section: Methodsmentioning

confidence: 99%

A New Gramicidin S Analogue with Potent Antibacterial Activity and Negligible Hemolytic Toxicity

Kalyvas,

Wang,

Toronjo-Urquiza

et al. 2024

J. Med. Chem.

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Antibiotic resistance is an urgent threat to global health, with the decreasing efficacy of conventional drugs underscoring the urgency for innovative therapeutic strategies. Antimicrobial peptides present as promising alternatives to conventional antibiotics. Gramicidin S is one such naturally occurring antimicrobial peptide that is effective against Staphylococcus aureus, with a minimum inhibitory concentration (MIC) of 4 μg/mL (3.6 μM). Despite this potent activity, its significant hemolytic toxicity restricts its clinical use to topical applications. Herein, we present rational modifications to the key β-strand and β-turn regions of gramicidin S to concurrently mitigate hemolytic effects, while maintaining potency. Critically, peptide 9 displayed negligible hemolytic toxicity, while possessing significant antibacterial potency against a panel of methicillin-sensitive and methicillin-resistant S. aureus clinical isolates (MIC of 8 μg/mL, 7.2 μM). Given the substantial antibacterial activity and near absence of cytotoxicity, 9 presents as a potential candidate for systemic administration in the treatment of S. aureus bacteremia/sepsis.

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Section: Methodsmentioning

confidence: 99%

A New Gramicidin S Analogue with Potent Antibacterial Activity and Negligible Hemolytic Toxicity

Kalyvas,

Wang,

Toronjo-Urquiza

et al. 2024

J. Med. Chem.

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Evaluation of protein extraction protocols for MALDI-TOF Biotyper analysis of mycobacteria

Machnik,

Smoliński,

Paściak

2024

Journal of Microbiological Methods

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Application of Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

KWON

2023

Korean J Clin Lab Sci

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The timeliness and accuracy of test results are crucial factors for clinicians to decide and promptly administer effective and targeted antimicrobial therapy, especially in life-threatening infections or when vital organs and functions, such as sight, are at risk. Further research is needed to refine and optimize matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based assays to obtain accurate and reliable results in the shortest time possible. MALDI-TOF MS-based bacterial identification focuses primarily on techniques for isolating and purifying pathogens from clinical samples, the expansion of spectral libraries, and the upgrading of software. As technology advances, many MALDI-based microbial identification databases and systems have been licensed and put into clinical use. Nevertheless, it is still necessary to develop MALDI-TOF MS-based antimicrobial-resistance analysis for comprehensive clinical microbiology characterization. The important applications of MALDI-TOF MS in clinical research include specific application categories, common analytes, main methods, limitations, and solutions. In order to utilize clinical microbiology laboratories, it is essential to secure expertise through education and training of clinical laboratory scientists, and database construction and experience must be maximized. In the future, MALDI-TOF mass spectrometry is expected to be applied in various fields through the use of more powerful databases.

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Improved MALDI-TOF MS Identification of Mycobacterium tuberculosis by Use of an Enhanced Cell Disruption Protocol

Cited by 4 publications

References 28 publications

A New Gramicidin S Analogue with Potent Antibacterial Activity and Negligible Hemolytic Toxicity

A New Gramicidin S Analogue with Potent Antibacterial Activity and Negligible Hemolytic Toxicity

Evaluation of protein extraction protocols for MALDI-TOF Biotyper analysis of mycobacteria

Application of Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

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