Improved mechanical properties by modifying fibrin scaffold with PCL and its biocompatibility evaluation

Lei, Yang; Li, Xiafei; Wang, Dongmei; Mu, Songfeng; Lv, Wenhao; Hao, Yongwei; Lu, Xiaosheng; Zhang, Guojiang; Nan, Wenbin; Chen, Hongli; Xie, Liqin; Zhang, Yongjun; Dong, Yuzhen; Zhang, Qiqing; Zhao, Liang

doi:10.1080/09205063.2019.1710370

Cited by 44 publications

(34 citation statements)

References 34 publications

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“…In previous paper published by our research group, the measurement of mechanical properties such as maximum stress, elastic modulus and breaking strain have been reported in detail [ 19 ]. To test the mechanical strength of the vascular graft, it was cut into 3 mm (width) × 4 mm (length) × 0.3 mm (thickness) samples respectively and fixed on a microcomputer controlled electronic universal testing machine (Kesheng WWD-100D, Jinan, China) for measurement.…”

Section: Methodsmentioning

confidence: 99%

“…In the previous work, we successfully prepared the PCL/fibrin vascular scaffold through electrospinning technology, and found that it had good biomechanical properties, cell compatibility, biocompatibility and degradation properties [ 19 ].Experiments results have confirmed that the PCL/fibrin scaffold material can be an ideal scaffold material for small diameter tissue engineering blood vessels. So we hypothesize that PCL/fibrin vascular scaffold is suitable as a substitute for artificial blood vessels in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Zhao

Lei

et al. 2021

Materials Science and Engineering: C

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The success of artificial vascular graft in the host to obtain functional tissue regeneration and remodeling is a great challenge in the field of small diameter tissue engineering blood vessels. In our previous work, poly(ε-caprolactone) (PCL)/fibrin vascular grafts were fabricated by electrospinning. It was proved that the PCL/fibrin vascular graft was a suitable small diameter tissue engineering vascular scaffold with good biomechanical properties and cell compatibility. Here we mainly examined the performance of PCL/fibrin vascular graft in vivo. The graft showed randomly arranged nanofiber structure, excellent mechanical strength, higher compliance and degradation properties. At 9 months after implantation in the rat abdominal aorta, the graft induced the regeneration of neoarteries, and promoted ECM deposition and rapid endothelialization. More importantly, the PCL/fibrin vascular graft showed more microvessels density and fewer calcification areas at 3 months, which was beneficial to improve cell infiltration and proliferation. Moreover, the ratio of M2/M1macrophage in PCL/fibrin graft had a higher expression level and the secretion amount of pro-inflammatory cytokines started to increase, and then decreased to similar to the native artery. Thus, the electrospun PCL/fibrin tubular vascular graft had great potential to become a new type of artificial blood vessel scaffold that can be implanted in vivo for long term.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Zhao

Lei

et al. 2021

Materials Science and Engineering: C

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“…The animal model of skin trauma in Sprague Dawley rats (SD rats) was selected to investigate the effect between QCT–TTO–pADM (cross-linked pADM when the concentration of QCT was 5 mg/mL) and wound through wound healing observation, histology observation, and immunohistochemical analysis. 50 …”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Antibacterial Porcine Acellular Dermal Matrices with High Performance

Wang

Gong

et al. 2020

ACS Omega

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Infection is a common complication in the process of wound management. An ideal wound dressing is supposed to reduce or even prevent the infection while promoting wound healing. A porcine acellular dermal matrix (pADM) has been already used as a wound dressing in clinic due to its capacity to accelerate wound healing. However, not only is pure pADM not antibacterial, its mechanical properties are poor. In this study, an antibacterial pADM with good performance was prepared by adding two natural products as modifiers, quercetin (QCT) and tea tree oil (TTO). The result of Fourier-transform infrared (FTIR) proved that the addition of modifiers did not break the natural triple-helical structure of collagen. Meanwhile, the results of differential scanning calorimetry (DSC), thermogravimetric analysis (TG), mechanic experiment, and enzymatic degradation demonstrated that pADM handled with QCT and TTO (termed QCT–TTO–pADM) had better thermal stability, mechanical strength, and resistance to enzymatic degradation than pADM. Meanwhile, QCT–TTO–pADM had excellent antibacterial activity and showed an antibacterial rate of over 80%. Furthermore, in the cytocompatibility analysis, QCT–TTO–pADM had no side effects on the adhesion, growth, and proliferation of fibroblasts. QCT–TTO–pADM could even accelerate wound healing more efficiently than pADM and glutaraldehyde-modified pADM (GA-pADM). In conclusion, QCT–TTO–pADM was a potential antibacterial wound dressing with good performance.

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“…Indeed, the average burst pressure of fibrin-based vascular grafts was 543 ± 77 mmHg, which is very low to withstand the physiological burst pressures of blood flow [125]. A recent study from Yang et al [126] showed that the mechanical properties of these vessel conduits can be improved with the addition of PCL, resulting in the production of a hybrid graft (fibrin-PCL vascular graft). In this study, electrospun PCL/fibrin vascular grafts were developed, followed by evaluation of mechanical properties, cytotoxic effects, and in vivo biocompatibility [126].…”

Section: Biopolymersmentioning

confidence: 99%

“…A recent study from Yang et al [126] showed that the mechanical properties of these vessel conduits can be improved with the addition of PCL, resulting in the production of a hybrid graft (fibrin-PCL vascular graft). In this study, electrospun PCL/fibrin vascular grafts were developed, followed by evaluation of mechanical properties, cytotoxic effects, and in vivo biocompatibility [126]. The burst pressure of these hybrid vascular grafts was 1811 ± 101 mmHg, which is similar to native blood vessels (2000 mmHg).…”

Section: Biopolymersmentioning

confidence: 99%

Future Perspectives in Small-Diameter Vascular Graft Engineering

et al. 2020

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The increased demands of small-diameter vascular grafts (SDVGs) globally has forced the scientific society to explore alternative strategies utilizing the tissue engineering approaches. Cardiovascular disease (CVD) comprises one of the most lethal groups of non-communicable disorders worldwide. It has been estimated that in Europe, the healthcare cost for the administration of CVD is more than 169 billion €. Common manifestations involve the narrowing or occlusion of blood vessels. The replacement of damaged vessels with autologous grafts represents one of the applied therapeutic approaches in CVD. However, significant drawbacks are accompanying the above procedure; therefore, the exploration of alternative vessel sources must be performed. Engineered SDVGs can be produced through the utilization of non-degradable/degradable and naturally derived materials. Decellularized vessels represent also an alternative valuable source for the development of SDVGs. In this review, a great number of SDVG engineering approaches will be highlighted. Importantly, the state-of-the-art methodologies, which are currently employed, will be comprehensively presented. A discussion summarizing the key marks and the future perspectives of SDVG engineering will be included in this review. Taking into consideration the increased number of patients with CVD, SDVG engineering may assist significantly in cardiovascular reconstructive surgery and, therefore, the overall improvement of patients’ life.

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Improved mechanical properties by modifying fibrin scaffold with PCL and its biocompatibility evaluation

Cited by 44 publications

References 34 publications

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo

Preparation and Characterization of Antibacterial Porcine Acellular Dermal Matrices with High Performance

Future Perspectives in Small-Diameter Vascular Graft Engineering

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