Improved Metagenomic Taxonomic Profiling Using a Curated Core Gene-Based Bacterial Database Reveals Unrecognized Species in the Genus Streptococcus

Abstract

Shotgun metagenomics is of great importance in order to understand the composition of the microbial community associated with a sample and the potential impact it may exert on its host. For clinical metagenomics, one of the initial challenges is the accurate identification of a pathogen of interest and ability to single out that pathogen within a complex community of microorganisms. However, in absence of an accurate identification of those microorganisms, any kind of conclusion or diagnosis based on misidentification may lead to erroneous conclusions, especially when comparing distinct groups of individuals. When comparing a shotgun metagenomic sample against a reference genome sequence database, the classification itself is dependent on the contents of the database. Focusing on the genus Streptococcus, we built four synthetic metagenomic samples and demonstrated that shotgun taxonomic profiling using the bacterial core genes as the reference database performed better in both taxonomic profiling and relative abundance prediction than that based on the marker gene reference database included in MetaPhlAn2. Additionally, by classifying sputum samples of patients suffering from chronic obstructive pulmonary disease, we showed that adding genomes of genomospecies to a reference database offers higher taxonomic resolution for taxonomic profiling. Finally, we show how our genomospecies database is able to identify correctly a clinical stool sample from a patient with a streptococcal infection, proving that genomospecies provide better taxonomic coverage for metagenomic analyses.

Comparative metagenomics reveals expanded insights into intra- and interspecific variation among wild bee microbiomes

“…Future studies which sample microbiomes from consistent life stages and tissue types across a comparably wide range of bee families may yield refined insights. We are on the cusp of tackling these and other questions as metagenomic methods continue to improve 91 and comparable metagenomic studies are performed in bees across independent origins of sociality 92 , 93 . These works promise to provide additional datasets that will be invaluable for further illuminating comparisons and that should contribute to a progressively well-defined spectrum of environmental through intranidal origins of bee metagenomes.…”

Metagenomic Association Analysis of Gut Symbiont Limosilactobacillus reuteri Without Host-Specific Genome Isolation

“…Species-level taxonomy profiles were created using a curated core gene-based bacterial database ( Chalita et al, 2020 ). All samples not containing L. reuteri in their profiles were discarded.…”

Metagenomic association analysis of gut symbiontLactobacillus reuteriwithout host-specific genome isolation

“…Species-level taxonomy profiles were created using a curated core gene-based bacterial database [32]. All samples not containing L. reuteri in their profiles were discarded.…”