Improved method for harvesting human Schwann cells from mature peripheral nerve and expansion in vitro

Casella, Gizelda; Bunge, Richard P.; Wood, Patrick M.

doi:10.1002/(sici)1098-1136(199608)17:4<327::aid-glia7>3.0.co;2-w

Cited by 165 publications

(95 citation statements)

References 24 publications

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“…All shipments were received and processing began within 12 hours of death. The procedure described by Casella (Casella et al, 1996) was utilized to isolate nhSCs from the nerves. Under these procedures it is apparent that the Schwann cells survive in a healthy state, and that the number of cells harvested from the nerve is equal or greater than the number of Schwann cells originally present in the nerve.…”

Section: Cell Culturementioning

confidence: 99%

Transcriptional profiling in an MPNST-derived cell line and normal human Schwann cells

et al. 2004

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AbstractcDNA microarrays were utilized to identify abnormally expressed genes in a malignant peripheral nerve sheath tumor (MPNST)-derived cell line, T265, by comparing the mRNA abundance profiles with that of normal human Schwann cells (nhSCs). The findings characterize the molecular phenotype of this important cell-line model of MPNSTs, and elucidate the contribution of Schwann cells in MPNSTs. In total, 4608 cDNA sequences were screened and hybridizations replicated on custom cDNA microarrays. In order to verify the microarray data, a large selection of differentially expressed mRNA transcripts were subjected to semi-quantitative reverse transcription PCR (LightCycler). Western blotting was performed to investigate a selection of genes and signal transduction pathways, as a further validation of the microarray data. The data generated from multiple microarray screens, semi-quantitative RT-PCR and Western blotting are in broad agreement. This study represents a comprehensive gene-expression analysis of an MPNST-derived cell line and the first comprehensive global mRNA profile of nhSCs in culture. This study has identified ~900 genes that are expressed abnormally in the T265 cell line and detected many genes not previously reported to be expressed in nhSCs. The results provide crucial information on the T265 cells that is essential for investigation using this cell line in experimental studies in neurofibromatosis type I (NF1), and important information on normal human Schwann cells that is applicable to a wide range of studies on Schwann cells in cell culture.

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Section: Cell Culturementioning

confidence: 99%

Transcriptional profiling in an MPNST-derived cell line and normal human Schwann cells

et al. 2004

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“…We identified CB as potent compound to induce neural cell identity and developed a CB-based approach to efficiently generate Schwann cells without any genetic modifications. Compared to existing Schwann cell sources like derivation from pluripotent stem cells or as primary cells (Casella et al, 1996), chemical-based conversion is more cost effective and easier to control and to standardize. Because Schwann cells play an important role in development, homeostasis, and diseases of the peripheral nervous system (Bhatheja and Field, 2006), we suggest that iSCs could represent useful tools to analyze Schwann cell functions or pathophysiology in vitro and to develop cellular interaction models allowing to study disease mechanisms in neuron-Schwann cell coculture.…”

Section: Stem Cell Reportsmentioning

confidence: 99%

Chemical Conversion of Human Fibroblasts into Functional Schwann Cells

Thoma

2018

Methods in Molecular Biology

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SUMMARYDirect transdifferentiation of somatic cells is a promising approach to obtain patient-specific cells for numerous applications. However, conversion across germ-layer borders often requires ectopic gene expression with unpredictable side effects. Here, we present a gene-free approach that allows efficient conversion of human fibroblasts via a transient progenitor stage into Schwann cells, the major glial cell type of peripheral nerves. Using a multikinase inhibitor, we transdifferentiated fibroblasts into transient neural precursors that were subsequently further differentiated into Schwann cells. The resulting induced Schwann cells (iSCs) expressed numerous Schwann cell-specific proteins and displayed neurosupportive and myelination capacity in vitro. Thus, we established a strategy to obtain mature Schwann cells from human postnatal fibroblasts under chemically defined conditions without the introduction of ectopic genes.

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“…208,209 Finally, Schwann cells can be obtained from adult peripheral nerve and rapidly expanded for autologous transplantation: human SCs have been successfully isolated, examined in vitro, and implanted into the injured rat spinal cord. [210][211][212] Schwann cell transplantation at the site of SCI in the adult rat enjoys a prolific history and has been reviewed previously. 213 Many experiments have tested a SC-filled PAN/PVC polymer channel, grafted at the site of acute thoracic transection in the adult rat.…”

Section: In the Spotlight: Schwann Cells And Oecsmentioning

confidence: 99%

Setting the stage for functional repair of spinal cord injuries: a cast of thousands

2005

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Here we review mechanisms and molecules that necessitate protection and oppose axonal growth in the injured spinal cord, representing not only a cast of villains but also a company of therapeutic targets, many of which have yet to be fully exploited. We next discuss recent progress in the fields of bridging, overcoming conduction block and rehabilitation after spinal cord injury (SCI), where several treatments in each category have entered the spotlight, and some are being tested clinically. Finally, studies that combine treatments targeting different aspects of SCI are reviewed. Although experiments applying some treatments in combination have been completed, auditions for each part in the much-sought combination therapy are ongoing, and performers must demonstrate robust anatomical regeneration and/or significant return of function in animal models before being considered for a lead role.Spinal Cord (2005) 43, 134-161.

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Improved method for harvesting human Schwann cells from mature peripheral nerve and expansion in vitro

Cited by 165 publications

References 24 publications

Transcriptional profiling in an MPNST-derived cell line and normal human Schwann cells

Transcriptional profiling in an MPNST-derived cell line and normal human Schwann cells

Chemical Conversion of Human Fibroblasts into Functional Schwann Cells

Setting the stage for functional repair of spinal cord injuries: a cast of thousands

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