Improved method for plasma malondialdehyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard

Sim, Ah Siew; Salonikas, Chris; Naidoo, Daya; Wilcken, D. E. L.

doi:10.1016/s1570-0232(02)00956-x

Cited by 121 publications

(111 citation statements)

References 16 publications

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“…The statistical tests were two-sided at a significant level α = 0.05. The level of the LPRRAs in healthy subjects were found to be 1.04±0.060, 1.26±0.28, 15.1±0.52 and 0.960±0.060 for GO, ACR, MDA and HNE, respectively, and these values show good agreement with other previous reports [12, [31][32][33][34].…”

Section: Analysis Of Serum Samples From Healthy Subjects and Patientssupporting

confidence: 90%

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

El-Maghrabey

Kishikawa

Ohyama

et al. 2014

Analytical Biochemistry

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A validated, simple and sensitive HPLC method was developed for the simultaneous determination of lipoperoxidation relevant reactive aldehydes; glyoxal (GO), acrolein (ACR), malondialdehyde (MDA) and 4-Hydroxy-2-nonenal (HNE) in human serum. The studied aldehydes were reacted with 2,2'-furil to form fluorescent difurylimidazole derivatives that were separated on respectively, with detection limits ranged from 0.030 to 0.11 nmol/mL. The % RSD of intra-day and inter-day precision didn't exceed 5.0% and 6.2%, respectively, and the accuracy (%found) ranged from 95.5 to 103%. The proposed method was applied for monitoring the four aldehydes in sera of healthy, diabetic and rheumatic human subjects with simple pretreatment steps and without interference from endogenous components. By virtue of its high sensitivity and accuracy, our method enabled detection of differences between analytes concentrations in sera of human subjects at different clinical conditions.

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Section: Analysis Of Serum Samples From Healthy Subjects and Patientssupporting

confidence: 90%

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

El-Maghrabey

Kishikawa

Ohyama

et al. 2014

Analytical Biochemistry

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“…MDA level was measured following a derivatization with 2,4-dinitrophenylhydrazine (DNPH) and HPLC analysis 13 with minor modifications. Fifty ml of reconstructed samples were injected into HPLC system (Agilent 1100 series) equipped with an autosampler, a quaternary pump, a UV diod-array detector, and a C18 column (Nucleodur AE C18 Pyramid 3 mmol/L, MachereyeNagel).…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Interaction of ω-3 polyunsaturated fatty acids with radiation therapy in two different colorectal cancer cell lines

et al. 2014

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Methods: LS174T and HT-29 cells were treated with 20 or 50 mmol/L EPA or DHA followed by single X-ray RT of 0, 2 or 4 Gy, to evaluate cell survival, apoptosis, peroxide and malondialdehyde productions. Inflammation-and apoptosis-related proteins were analyzed by Western Blot. ANOVAs were used for statistical analysis. Results: LS174T was more sensitive to RT than HT-29. DHA and to a lesser extent EPA increased cell death, apoptosis and peroxide production after RT in LS174T and to a lesser extent in HT-29 (p < 0.05). This was associated with increased expression of heat shock protein 70, decreased expression of NF-kB p65, COX-2 and Bcl-2 proteins. Conclusions: The effect of RT combination with DHA and to a lesser extent EPA was synergistic in the radio-sensitive LS174T cells, but additive in the radio-resistant HT-29 cells. This enhanced cytotoxicity was provoked at least partly by lipid peroxidation, which consequently modulated inflammatory response and induced apoptosis.

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“…[3,4] The reaction of MDA and TBA depends on temperature and pH, but too strong acid and high temperature (so-called "harsh conditions") can lead to overestimation of results due to artefactual peroxidation of sample constituents. [16] In this study the reaction mixture was heated at 90 °C for 30 minutes, since the experimental data indicate that these conditions yield maximal product formation (Figure 2). Literature references also indicate that the TBA assay lacks selectivity: TBA is reacting not only with MDA, but also with other components of biological samples, such as sugars, amino acids, bilirubin and albumin.…”

Section: Sample Preparation and Testing For Possible Interferencesmentioning

confidence: 99%

Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies

Weitner¹,

Inić²,

Jablan³

et al. 2016

Croat. Chem. Acta

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A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at -20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of "whole-body" oxidative stress.

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Improved method for plasma malondialdehyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard

Cited by 121 publications

References 16 publications

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

Analytical method for lipoperoxidation relevant reactive aldehydes in human sera by high-performance liquid chromatography–fluorescence detection

Interaction of ω-3 polyunsaturated fatty acids with radiation therapy in two different colorectal cancer cell lines

Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies

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