Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity

Schierwagen, Cecilia; Bylund-Fellenius, Ann-Christin; Lundberg, Claes

doi:10.1016/0160-5402(90)90061-o

Cited by 343 publications

(215 citation statements)

References 15 publications

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“…The carrageenin-induced cellular response was inhibited by a NK 1 receptor antagonist, SR140333, in the wild-type group. Furthermore, B 1 and B 2 receptor antagonists (desArg 9 [Leu 8 ]bradykinin and HOE 140) also attenuated neutrophil accumulation to carrageenin in wild-type mice but not in knockout mice. The results provide further evidence that B 1 and B 2 receptors participate in the NK 1 receptor-dependent component of the neutrophil cell recruitment induced by carrageenin.…”

Section: Discussionmentioning

confidence: 99%

Neurokinin-1 Receptor Agonists Are Involved in Mediating Neutrophil Accumulation in the Inflamed, But Not Normal, Cutaneous Microvasculature: An In Vivo Study Using Neurokinin-1 Receptor Knockout Mice

Cao¹,

Pintér²,

Al-Rashed³

et al. 2000

The Journal of Immunology

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We have used tachykinin neurokinin-1 receptor (NK1 receptor) knockout mice to learn of the link between NK1 receptors and neutrophil accumulation in normal naive skin, as compared with inflamed skin. Intradermal substance P (300 pmol) induced edema formation in wild-type mice, but not in NK1 knockout mice, as expected. However, in contrast to IL-1β (0.3 pmol), substance P did not induce neutrophil accumulation in wild-type mice. IL-1β-induced neutrophil accumulation was similar in wild-type and knockout mice, but a significant (p < 0.05) contributory effect of added NK1 agonists, which by themselves have no effect on neutrophil accumulation in normal skin, was observed. The results support the concept that NK1 agonists such as substance P cannot act on their own to mediate neutrophil accumulation in naive skin and provide direct evidence that in inflamed skin, under certain circumstances, the NK1 receptor can play a pivotal role in modulating neutrophil accumulation during the ongoing inflammatory process. We investigated responses to two inflammatory stimuli (carrageenin and zymosan). Neutrophil accumulation was significantly attenuated (p < 0.001) in carrageenin- but not zymosan-induced inflammation in NK1 knockout mice. The carrageenin (500 μg)-induced response was inhibited (p < 0.05) by a NK1 receptor antagonist, SR140333 (480 nmol/kg i.v. at −5 min), in the wild-type group. The bradykinin B1 and B2 receptor antagonists (desArg9[Leu8]bradykinin and HOE 140) each reduced neutrophil accumulation to carrageenin in wild-type animals (p < 0.05), but did not cause further reduction of the suppressed response of knockout mice. The results provide evidence that kinin receptors participate in NK1 receptor-dependent neutrophil accumulation in inflamed mouse skin.

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Section: Discussionmentioning

confidence: 99%

Neurokinin-1 Receptor Agonists Are Involved in Mediating Neutrophil Accumulation in the Inflamed, But Not Normal, Cutaneous Microvasculature: An In Vivo Study Using Neurokinin-1 Receptor Knockout Mice

Cao¹,

Pintér²,

Al-Rashed³

et al. 2000

The Journal of Immunology

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“…Lung MPO, as an indirect measurement of neutrophil infiltration, was quantified as described previously (15). Briefly, fresh lung samples were weighed (50 mg) and homogenized in 1 ml buffer A (3.4 mM KH 2 PO 4 , 16 mM Na 2 HPO 4, PH 7.4).…”

Section: Lung Myeloperoxidase (Mpo) Assaymentioning

confidence: 99%

Inefficiency of C3H/HeN Mice to Control Chlamydial Lung Infection Correlates with Downregulation of Neutrophil Activation During the Late Stage of Infection

Tang

Zhang

et al. 2009

Cell Mol Immunol

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We previously reported that massive infiltration of neutrophils in C3H/HeN (C3H) mice could not efficiently control Chlamydia muridarum (Cm) infection and might contribute to the high susceptibility of these mice to lung infection. To further define the nature of neutrophil responses in C3H mice during chlamydial infection, we examine the expression of adhesion molecules and CD11b related to neutrophils infiltration and activation, respectively, following intranasal Cm infection. The results showed that the expression of selectins (E-selectin, P-selectin and L-selectin), and intercellular cell adhesion molecule-1 (ICAM-1) in the lung of C3H mice increased more significantly than in C57BL/6 (B6) mice, the more resistant strain. These results correlated well with the massive neutrophils infiltration in C3H mice. In contrast, CD11b expression on peripheral blood and lung neutrophils in C3H mice exhibited a significant reduction compared with B6 mice during the late phage of infection (day 14). These findings suggest that the high-level expression of adhesion molecules in C3H mice may enhance neutrophils recruitment to the lung, but the decline of CD11b expression on neutrophils may attenuate neutrophil function. Therefore, CD11b down-regulation on neutrophils may contribute to the failure of C3H mice to control chlamydial lung infection. Cellular & Molecular Immunology. 2009;6(4):253-260.

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“…MPO reflects accumulation of tissue polymorphonuclear cells (PMN), as MPO in neutrophils is constant (55). In our study, we found that ACS resulted in significant increases of MPO, particularly in lung, liver and small intestine.…”

Section: Discussionmentioning

confidence: 58%

“…The pressure exerted on the abdominal boundaries will be directly related to the intraabdominal volume (IAV) and the compliance of the abdominal wall. Similar to other compartments, the pressure increases exponentially at a critical volume (55). Further, the pressures, but not the curve shape, are influenced by abdominal wall compliance, with low abdominal wall compliance leading to higher pressures at the same volume (56).…”

Section: Intra-abdominal Pressurementioning

confidence: 95%

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Intra-abdominal hypertension and the abdominal compartment syndrome

Schein

Ivatury

1998

Journal of British Surgery

138

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Intra-abdominal hypertension (IAH) and abdominal compartment syndrome (ACS) are increasingly recognized in critically ill patients; no nonoperative treatments exist, and mortality remains high.The purpose of this thesis was to prospectively characterize the incidence of IAH in a mixed medical-surgical intensive care unit, and to test the potential therapeutic benefit of carbon monoxide (CO) and hydrogen sulphide (H2S) using an animal model of ACS. IAH was diagnosed in 30% of patients on admission; further 15% developed IAH during ICU stay. Incidence of ACS was 3%, with obesity, sepsis, mechanical ventilation and 24-hour fluid balance as independent predictors, also predicting ICU mortality. In a rat model of ACS, CO and H2S were found to improve ACS-induced microcirculatory dysfunction, inflammation, cell death and overall organ dysfunction.

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Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity

Cited by 343 publications

References 15 publications

Neurokinin-1 Receptor Agonists Are Involved in Mediating Neutrophil Accumulation in the Inflamed, But Not Normal, Cutaneous Microvasculature: An In Vivo Study Using Neurokinin-1 Receptor Knockout Mice

Neurokinin-1 Receptor Agonists Are Involved in Mediating Neutrophil Accumulation in the Inflamed, But Not Normal, Cutaneous Microvasculature: An In Vivo Study Using Neurokinin-1 Receptor Knockout Mice

Inefficiency of C3H/HeN Mice to Control Chlamydial Lung Infection Correlates with Downregulation of Neutrophil Activation During the Late Stage of Infection

Intra-abdominal hypertension and the abdominal compartment syndrome

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