Improved method for the determination of sulphate in human serum using ion chromatography

Reiter, Christoph; Müller, Susanne; Muller, Thomas E.

doi:10.1016/0378-4347(87)80235-9

Cited by 17 publications

(3 citation statements)

References 16 publications

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“…Colorimetric methods using benzidine (9), barium chloranilate (10) or rhodizonate (11) as well as turbidimetric methods based on the detection of barium sulphate (12) have been described for the determination of inorganic sulphate in biological materials. The kinetic of sulphate consumption was approximated as c(t) = c x + (c 0 -c«) · e" The kinetic of acetaminophen was approximated as c(t) (16). Several slightly different methods for the determination of sulphate in serum by high-performance-ion-chromatography with conductivity detection have also been described (5, 13 -16).…”

Section: Discussionmentioning

confidence: 99%

The Determination of Inorganic Sulphate in Serum and Synovial Fluid by High Performance Ion Chromatography

Kock¹,

Schneider²,

Delvoux³

et al. 1997

Clinical Chemistry and Laboratory Medicine

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A method for the determination of inorganic sulphate based on high performance ion chromatography is presented. The separation was performed on an anion-exchange column with a 1.8mmol/l sodium carbonate/ 1.7 mmol/1 sodium hydrogen carbonate-buffer, pH 10.35. Conductivity of the eluate was monitored after suppression of the background conductivity caused by the eluent-buffer.Serum and synovial fluid samples were prepared by ultrafiltration through membranes with a molecular mass cutoff of M T 10000. The viscosity of the synovial fluids was reduced by treatment with hyaluronate lyase before ultrafiltration.The method showed a linear response for sulphate concentrations between 0.5 and 1000 μπιοΐ/ΐ. The limit of detection was 1 umol/l for aqueous standards. For serum the coefficient of variation within-run was 2.3%-2.4%, the coefficient of variation between days 2.9%-3.1%. For synovial fluids the coefficient of variation within-run was 3.1%-3.4%, the coefficient of variation between days 4.6%-5.7%. Standard recovery experiments performed by spiking pools of human sera containing low sulphate concentrations with sulphate concentrations between 5 μιηοΐ/ΐ and 40 μηιοΐ/ΐ showed recoveries between 98.9% and 100.6%. The corresponding experiments with pools of synovial fluids showed recoveries of 98.3% to 100.9%.As determined from 127 serum samples the reference range for sulphate was 262 μηιοΐ/ΐ-420 μπιοΐ/l, with a mean value of 314 μηιοΐ/ΐ. No dependence on age or sex was observed.The sulphate concentration in 36 synovial fluids from knees affected by inflammatory processes showed a mean value of 424 μπιοΐ/l and a standard deviation of 70 μπιοΐ/ΐ. In 41 synovial fluids from knees affected by chronic degenerative joint disease, the sulphate concentrations were statistically significantly lower, with a mean of 374 μπιοΙ/1 and a standard deviation of 58 μηιοΐ/ΐ. The concentrations of sulphate in the synovial fluids were statistically significantly higher than those in the serum samples used for determination of the reference range.Following the oral application of a subtoxic single dose of acetaminophen (32.5 mg/kg body weight-62.5 mg/kg body weight) to 4 healthy volunteers, there was a significant decrease in the concentration of sulphate in serum with a minimum at 4-5 h after application of the drug. The cumulative concentration decrease of sulphate in serum and the kinetic constant of the sulphate depletion were not correlated with the applied acetaminophen dose normalized for body weight.

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Section: Discussionmentioning

confidence: 99%

The Determination of Inorganic Sulphate in Serum and Synovial Fluid by High Performance Ion Chromatography

Kock¹,

Schneider²,

Delvoux³

et al. 1997

Clinical Chemistry and Laboratory Medicine

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“…To confirm the results obtained using the IC system proposed in this paper, the same samples were pre-treated (methods for pretreatment were the same as described in the literature 4,6 ) and then analysed using a conventional anion-exchange IC system. The concentration for Br 2 was 30.4 mM and for NO 3 2 was 421.1 mM in the urine sample.…”

Section: Determination Of Anions In Real Samplesmentioning

confidence: 67%

“…A number of techniques/methods for the pretreatment of biological samples have been developed. Mixing the sample with silver resin 4 to eliminate the chloride ion or addition of acetonitrile 5 or perchloric acid 6 to the sample and then centrifugation of the mixture to remove proteins are well known and frequently used methods.…”

Section: Introductionmentioning

confidence: 99%

Determination of inorganic anions in biological fluids with direct sample injection by electrostatic ion chromatography using zwitterionic micelles in both stationary and mobile phases

Tanaka

Hasebe

2000

Analyst

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A new ion chromatographic (IC) system, which uses zwitterionic (e.g., Zwittergent 3-14) micelles as both stationary and mobile phases, highly useful for the analysis of inorganic anions in biological samples, was developed. The zwitterionic micellar stationary phase (which is obtained by immobilizing the zwitterionic surfactant on surfaces of the reversed-phase ODS) showed high ability to confine the elution bands of the large amount of SO4(2-) and Cl- to narrow zones. As a result, a base-line separation of NO2-, Br- and NO3- from SO4(2-) and Cl- is always achieved. The zwitterionic micellar mobile phase, (which is obtained by dissolving the zwitterionic surfactant with a suppressive electrolytic solution, e.g., aqueous NaHCO3 solution), on the other hand, showed high ability for rapid elution of proteins. The separation column is therefore always being cleaned up even after the protein-containing sample is directly injected. The zwitterionic micelles are also insensitive to conductivity detection, therefore either the suppressed or the non-suppressed conductivity detection method is applicable for detection of the analyte ions. Urine and serum were chosen as the model real samples and were analysed with direct sample injection; the results of successful determination of a number of inorganic anions (SO4(2-), Cl-, NO2-, Br- and NO3-) in both samples have demonstrated the usefulness of this new IC system.

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1994

Ion Chromatography

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Improved method for the determination of sulphate in human serum using ion chromatography

Cited by 17 publications

References 16 publications

The Determination of Inorganic Sulphate in Serum and Synovial Fluid by High Performance Ion Chromatography

The Determination of Inorganic Sulphate in Serum and Synovial Fluid by High Performance Ion Chromatography

Determination of inorganic anions in biological fluids with direct sample injection by electrostatic ion chromatography using zwitterionic micelles in both stationary and mobile phases

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