Improved methodology for analysis of multiple phytohormones using sequential magnetic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry

Luo, Xiao-Tong; Cai, Bingna; Chen, Xi; Feng, Yu‐Qi

doi:10.1016/j.aca.2017.06.019

Cited by 38 publications

(24 citation statements)

References 32 publications

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“…Compared with classic extraction methods, microextraction methods have the advantages of having high extraction efficiency and miniaturization of device and enrichment capacity making them more suitable for the analysis of plant hormone [47]. The extraction and clean-up techniques for trace compounds analysis include liquid-liquid extraction (LLE) [17,21,24,30,[63][64][65], liquid phase microextraction (LPME) [52,56,66], dispersive liquid-liquid microextraction (DLLME) [40,67], polymer monolith microextraction (PMME) [68], solid phase extraction (SPE) [26,38,55,[69][70][71][72], solid phase microextraction (SPME) [25,34], magnetic solid phase extraction (MSPE) [16,23,73], matrix solidphase dispersion (MSPD) [37], dispersive micro solid phase extraction (DMSPE) [74], ion pair stir bar sorptive extraction (SBSE) [1] and electromembrane extraction (EME) [75] (see Fig. 1).…”

Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

“…Over the past decades, the trend of plant hormone analysis has lied in a tiny amount sample consumption with sensitive performance [35,115,116]. However, most of the plant hormones were present in plant at ultra-low levels, while many classed of them exhibit with low ionization efficiency in MS [16,40]. Benefit from high resolution of MS, chemical labeling may serve as a strong tool to enhance sensitivity and accuracy of determining plant hormones [11,26], which overcome the problem by synthesizing series of stable isotope-coded reagents for chemical derivatization to the target compounds during sample pretreatment or MS detection procedure [117].…”

Section: Chemical Labelingmentioning

confidence: 99%

“…Good precisions with RSD 1.5-13.8% (intra-day) and 1.2-7.3% (inter-day) and acceptable recoveries (88.6-102.9%, n = 6) were achieved through this method. Luo et al [16] developed a method for analyzing plant hormones including IAA, ABA, JA, GAs, CKs and BRs in 100 mg (FW) of Brassica napus L. The samples were purified and separated by sequential magnetic solid-phase extraction (MSPE) and then analyzed by an ultra-high performance liquid chromatographytandem mass spectrometry (UHPLC-MS/MS) with the LOD (S/N = 3) ranging from 0.45 to 126.1 pg mL −1 . The amount of plant tissues samples required has been reduced significantly from g (FW) to mg (FW).…”

Section: Acidic Plant Hormones Auxin Abscisic Acid (Aba) Jasmonic Amentioning

confidence: 99%

“…Accurate determination of plant hormones requires the development of highly sensitive and efficient analytical techniques for analysis of plant hormone. Effective analysis of plant hormones is limited by inefficient extraction procedures due to their extremely low abundance (usually at ng g −1 or even pg g −1 level) and varied concentration ranges in plants [16]. For instance, the concentration of auxin and JA in plants ranges about 1-50 ng g −1 fresh weight (FW) whereas the content of BRs is as low as 0.01-0.1 ng g −1 FW [17].…”

Section: Introductionmentioning

confidence: 99%

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Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

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Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

Section: Chemical Labelingmentioning

confidence: 99%

Section: Acidic Plant Hormones Auxin Abscisic Acid (Aba) Jasmonic Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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“…Graphene oxide (GO) is a novel carbon material and contains a large number of epoxy groups, phenolic hydroxyl groups and carboxyl groups, and other oxygen-containing functional groups, and its electron-rich double-sided polycyclic aromatic scaffold and large surface area makes it a promising analytical candidate as a novel and high-potential SPE adsorbent [21,[27][28][29][30][31]. GO-based MSPE methods have been used to enrich various compounds such as metal ions, molecules of biological interest, medical products, and environmental and food contaminants for environmental analysis, food safety analysis, and bioanalysis [21,[28][29][30][31], and recent reports have demonstrated that the GO-MSPE method was an outstanding sample preparation technique in the separation and enrichment of phytohormones from complex plant samples [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide composites for magnetic solid‐phase extraction of trace cytokinins in plant samples followed by liquid chromatography–tandem mass spectrometry

Cheng

Qin

et al. 2018

J of Separation Science

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In this work, an easy, effective, and sensitive method based on graphene oxide@silica@magnetite composites as adsorbent of magnetic solid-phase extraction combined with liquid chromatography and tandem mass spectrometry, was established and validated for the trace analysis of cytokinins in different plants. The prepared magnetic composite was characterized by infrared spectroscopy, transmission electron microscopy, Brunauer-Emmett-Teller analysis, and magnetic hysteresis. Under the optimized conditions, good linearities in the range of 0.5-100 ng/mL were obtained with the corresponding linear correlation coefficient >0.9989 for the investigated four cytokinins, and good sensitivity levels were achieved with low detection limits ranging from 93 to 120 pg/mL. The established magnetic solid-phase extraction with liquid chromatography and tandem mass spectrometry method has been validated in the separation and analysis of four cytokinins in plant samples with good recoveries between 78.9 and 97.3% for four cytokinins with the relative standard deviations lower than 13.5%.

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Recent advances in application and progress of advanced materials as adsorbents in sample preparation for plant growth regulators

Liu

2023

J of Separation Science

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Plant growth regulators are a class of physiologically active substances that could modify or regulate basic physiological processes in the plant and defense against abiotic and biotic stresses, including natural plant growth regulators and synthetic ones. Different from natural plant growth regulators with low content and high cost of extraction in plants, synthetic ones can be produced in large‐scale production and widely used in agriculture for increasing and securing yield and quality of the harvested produce. However, like pesticides, the abuse of plant growth regulators will have negative impacts on human beings. Therefore, it is important to monitor plant growth regulators residues. Due to the low concentration of plant growth regulators and complex matrices of food, it is necessary to isolate and extract plant growth regulators by appropriate adsorbents in sample preparation for obtaining satisfactory results. In the last decade, several advanced materials as adsorbents have shown superiority in sample preparation. This review briefly introduces the recent application and progress of advanced materials as adsorbents in sample preparation for extraction of plant growth regulators from the complex matrix. In the end, the challenge and outlook about the extraction of plant growth regulators of these advanced adsorbents in sample preparation are presented.

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Improved methodology for analysis of multiple phytohormones using sequential magnetic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry

Cited by 38 publications

References 32 publications

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Graphene oxide composites for magnetic solid‐phase extraction of trace cytokinins in plant samples followed by liquid chromatography–tandem mass spectrometry

Recent advances in application and progress of advanced materials as adsorbents in sample preparation for plant growth regulators

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