Improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells

Goddeeris, Bruno; Baldwin, Cynthia L.; ole-MoiYoi, Onesmo K.; Morrison, W. Ivan

doi:10.1016/0022-1759(86)90354-6

Cited by 100 publications

(52 citation statements)

References 28 publications

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“…Monocytes were isolated from PBMC by using gelatine and plasma-coated flasks according to the method of Goddeeris et al (1986) with minor modifications. Whole PBMC were added to gelatine\plasma-coated flasks for 2 h at 37 mC, after which the non-adherent cell populations were removed by gentle swirling and rinsing with PBS.…”

Section: Methodsmentioning

confidence: 99%

Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.

Rhodes

Cocksedge

Collins

et al. 1999

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.

Rhodes

Cocksedge

Collins

et al. 1999

Journal of General Virology

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“…Blood was either collected into a solution of heparin or defibrinated as indicated for the assay (24). PBMC were isolated from blood via density gradient centrifugation over Ficoll-Hypaque (Ficoll-Paque; LKB-Pharmacia Biotechnology) by standard techniques.…”

Section: Animals and Cellsmentioning

confidence: 99%

γδ T Cell Function Varies with the Expressed WC1 Coreceptor

Rogers¹,

VanBuren

Hedblom³

et al. 2005

The Journal of Immunology

115

107

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WC1 molecules are transmembrane glycoproteins belonging to the scavenger receptor cysteine-rich family and uniquely expressed on γδ T cells. Although participation of WC1+ γδ T cells in immune responses is well established, very little is understood regarding the significance of expressing different forms of the WC1 molecule. Two forms previously identified by mAbs, i.e., WC1.1 and WC1.2, are expressed by largely nonoverlapping subpopulations of γδ T cells. In this study it was shown that expression of the WC1.1 coreceptor was the main indicator of proliferation and IFN-γ production in response to autologous and bacterial Ags as well as for IFN-γ production without proliferation in Th1-polarizing, IL-12-containing cultures. Nevertheless, after culture in either Th1-polarizing or neutral conditions, mRNA was present for both T-bet and GATA-3 as well as for IL-12Rβ2 in WC1.1+ and WC1.2+ subpopulations, and neither produced IL-4 under any conditions. Although the steady decrease in the proportion of WC1.1+ cells, but not WC1.2+ cells, within PBMC with animal aging suggested that the two subpopulations may have different roles in immune regulation, cells bearing either WC1.1 or WC1.2 expressed mRNA for regulatory cytokines IL-10 and TGF-β, with TGF-β being constitutively expressed by ex vivo cells. Overall, the results demonstrate that the form of the WC1 coreceptor expressed on γδ T cells divides them into functional subsets according to IFN-γ production and proliferative capacity to specific stimuli as well as with regard to representation within PBMC. Finally, evidence is provided for minor differences in the intracytoplasmic tail sequences of WC1.1 and WC1.2 that may affect signaling.

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“…Importantly, this latter finding suggests that, upon enrichment, T cells become resistant to apoptosis induced by the cytopathic virus. To determine whether the presence of monocytes is required to trigger apoptosis in T cells, we performed mixing experiments in which monocytes were added, at different concentrations, to non-adherent cells isolated from PBMC by a plasma\gelatine method (Goddeeris et al, 1986). We showed that the percentages of BoCD4 + and BoCD8 + T cells undergoing apoptosis in cytopathic virus-containing medium were consistently greater in the presence of autologous monocytes than those observed without addition of monocytes (Fig.…”

mentioning

confidence: 96%

Bovine viral diarrhoea virus induces apoptosis in blood mononuclear cells by a mechanism largely dependent on monocytes.

Lambot

Hanon

Lecomte

et al. 1998

Journal of General Virology

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Improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells

Cited by 100 publications

References 28 publications

Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.

Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.

γδ T Cell Function Varies with the Expressed WC1 Coreceptor

Bovine viral diarrhoea virus induces apoptosis in blood mononuclear cells by a mechanism largely dependent on monocytes.

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