Improved methods for the extraction and analysis of isoflavones from soy-containing foods and nutritional supplements by reversed-phase high-performance liquid chromatography and liquid chromatography–mass spectrometry

Griffith, Aaron; Collison, Mark W.

doi:10.1016/s0021-9673(00)01077-3

Cited by 189 publications

(132 citation statements)

References 20 publications

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“…C. The area ratios of daidzein, genistein and glycitein against the area of the internal standard were obtained from a chromatogram, and the plasma concentrations of daidzein, genistein and glycitein were obtained from a quantity calculation. 29) Pharmacokinetic analysis. The non-compartment model of the WinNonlin program (Pharsight, USA) was used to calculate the AUC values of IFE and IFE--CD.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Bioavailability of Soy Isoflavones by Complexation with β-Cyclodextrin in Rats

Lee

Kim

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Enhanced Bioavailability of Soy Isoflavones by Complexation with β-Cyclodextrin in Rats

Lee

Kim

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…Serum isoflavones were analyzed by the National Center for Toxicological Research in Jefferson, AR using HPLC-UV [16]. The limits of detection of genistein, daidzein, and equol were 0.004, 0.002, and 0.03 µM respectively.…”

Section: Serum Isoflavone Analysismentioning

confidence: 99%

Pelleting Diets Impairs TRAMP Prostate Carcinogenesis

Conlon¹,

Wallig²,

Erdman³

2017

FNS

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Diets rich in soy products may reduce the risk of prostate cancer (PCa). Daidzein, the major isoflavone present in soy germ, can be metabolized by the gut microbiota into equol. The effects of daidzein and equol on PCa have not been well studied. The objective of this study was to investigate the effect of feeding 2% soy germ, 92 ppm daidzein, or 88 ppm equol diets on the progression of PCa in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. 3-week old male C57BL/6 X FVB TRAMP mice were weaned from our breeding colony and immediately acclimated to an AIN-93G control diet for one week. At 4 weeks of age, mice (n = 30 per diet group) were randomized to one of four pelleted study diets until 18 weeks of age. Unexpectedly, we did not detect any statistical differences in cancer incidence between diets. We suggest that these results are due to the physical attributes of the pelleted diets in the current study. Mice fed pelleted diets had reduced food intake and significantly decreased body weights (p < 0.001) compared to previous studies. A reduction in food intake is known to reduce cancer incidence in a number of cancer models and is likely to have contributed to the decrease in expected cancer incidence in the current study. In conclusion, we suggest that the hardness of the diets pellets could result in a decreased cancer incidence in TRAMP mice.

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“…Initial chromatographic conditions were based on those described in related publications (Adlercreutz, 2003;Griffith & Collison, 2001;Wu et al, 2004;Klejdus et al, 2005), which described methods of quantification of soy isoflavones by liquid chromatography. Following the procedures of these studies, trials were conducted to develop the mobile phase.…”

Section: Instrument and Hplc Conditionsmentioning

confidence: 99%

“…Many articles have been written on the identification and quantification of isoflavones or phytoestrogens (Wang et al, 2002) in soy products (such as capsules of soy germ, and nutritional supplements or other products containing soy) using high-performance liquid chromatography (HPLC) detection method (Micke et al, 2006;Griffith & Collison, 2001), nuclear magnetic resonance spectroscopy (Xiao et al, 2005), and other chromatographic. However, only a few researchers (such as César et al, 2006) have developed methods for quantifying isoflavones in capsules.…”

Section: Introductionmentioning

confidence: 99%

Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography

Auwerter¹,

Wanczinski²,

Chiandotti³

2012

Rev. bras. farmacogn.

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Isoflavones can be found in grains and leaves of soybean. Currently, these are sold in pharmacies as phytotherapic capsules. Isoflavones have been recommended by doctors, especially for women, due to their ability to relieve menopause symptoms, among other benefits. However, no method exists for the official control of isoflavone content in capsules sold in the Brazilian market. This study aims to develop an appropriate analytical method to determine the total isoflavone content (daidzin, glycitin, and genistin, and their respective aglycone forms) in phytotherapic capsules purchased in pharmacies in Curitiba, Parana State, Brazil, using the technique of high-performance liquid chromatography with UV detection (UV-HPLC). The HPLC system consisted of a quaternary pump, an autosampler, and Waters reversed-phase C 18 column (5 μm × 300 mm. Analyses were carried out at 40 °C, using a flow rate of 1.0 mL/min (acetonitrile and acetic acid 0.1%), and detection was performed at 254 nm. The method was validated as required by ANVISA and showed to be reliable for the following parameters: linearity (r 2 >0.99), selectivity (correlation between 0.99 and 1.00), precision (relative standard derivation <1.59%), accuracy (from 80% to 111.63% intraday and from 80% to 117.88% interday recovery), and robustness.

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Improved methods for the extraction and analysis of isoflavones from soy-containing foods and nutritional supplements by reversed-phase high-performance liquid chromatography and liquid chromatography–mass spectrometry

Cited by 189 publications

References 20 publications

Enhanced Bioavailability of Soy Isoflavones by Complexation with β-Cyclodextrin in Rats

Enhanced Bioavailability of Soy Isoflavones by Complexation with β-Cyclodextrin in Rats

Pelleting Diets Impairs TRAMP Prostate Carcinogenesis

Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography

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