Improved microbial oil production from oil palm empty fruit bunch by Mucor plumbeus

Ahmad, Farah B.; Zhang, Zhanying; Doherty, William O.S.; Te’o, Junior; O’Hara, Ian M.

doi:10.1016/j.fuel.2017.01.013

Cited by 24 publications

(11 citation statements)

References 30 publications

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“…The molasses medium contained a sugar concentration of ~30 g L -1 (glucose equivalent), 0.5 g L -1 (NH 4 ) 2 SO 4 and 0.25 g L -1 KH 2 PO 4 . Fungal biomass was produced at 28 °C, pH 6.0 over a cultivation period of 6 days with oxygen levels maintained at above 20% by aeration (Ahmad et al, 2017). Microbial oil production was performed under nitrogen-limiting condition.…”

Section: Fungal Biomass Cultivation and Validation Of T 2 Fd-induced Cell Breakagementioning

confidence: 99%

Turbo thin film continuous flow production of biodiesel from fungal biomass

Sitepu

Jones

Zhang

et al. 2019

Bioresource Technology

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Direct biodiesel production from wet fungal biomass may significantly reduce production costs, but there is a lack of fast and cost-effective processing technology. A novel thin film continuous flow process has been applied to study the effects of its operational parameters on fatty acid (FA) extraction and FA to fatty acid methyl ester (FAME) conversion efficiencies. Single factor experiments evaluated the effects of catalyst concentration and water content of biomass, while factorial experimental designs determined the interactions between catalyst concentration and biomass to methanol ratio, flow rate, and rotational speed. Direct transesterification (DT) of wet Mucor plumbeus biomass at ambient temperature and pressure achieved a FA to FAME conversion efficiency of > 90% using 3 wt./v % NaOH concentration, if the water content was ≤ 50% (w/w). In comparison with existing DT methods, this continuous flow processing technology has an estimated 90-94% reduction in energy consumption, showing promise for up-scaling.

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Section: Fungal Biomass Cultivation and Validation Of T 2 Fd-induced Cell Breakagementioning

confidence: 99%

Turbo thin film continuous flow production of biodiesel from fungal biomass

Sitepu

Jones

Zhang

et al. 2019

Bioresource Technology

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“…The selection of types of microorganism is crucial as different microorganisms could accumulate different amount lipids. The lipid accumulation could be optimized through optimizing the cultivation media [33], as it will impact glycolysis pathway and microbial growth (subsequently biomass yield). However, this is not the focus of this study as the aim of this study is to optimize the main economic bottleneck for microbial biodiesel production, which is the extraction and transesterification.…”

Section: Microbial Biodiesel Production Via In-situ Transesterification Of Fungal Biomassmentioning

confidence: 99%

Comparison of Ex-Situ and In-Situ Transesterification for the Production of Microbial Biodiesel

Hazmi

Ahmad

et al. 2021

Bull. Chem. React. Eng. Catal.

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Microbial biodiesel is converted from microbial lipids via transesterification process. Most microbial biodiesel studies are focusing on the use of microalgal lipids as feedstock. Apart from using microalgae for lipid biosynthesis, lipids can also be extracted from other oleaginous microorganisms like fungi and yeast. However, there are gaps in the studies of lipid production from filamentous fungi, especially in-situ transesterification process. The aim of this project is to compare in-situ with the ex-situ transesterification of fungal biomass from Aspergillus oryzae. In ex-situ transesterification, two methods of lipid extraction, the Soxhlet extraction and the Bligh and Dyer extraction, were performed. For in-situ transesterification, two methods using different catalysts were investigated. Base-catalyzed in-situ transesterification of fungal biomass resulted on the highest Fatty Acid Methyl Esters (FAME) yield. The base-catalyzed in-situ transesterification was further optimized via Central Composite Design (CCD) of Response Surface Methodology (RSM). The parameters investigated were the catalyst loading, methanol to biomass ratio and reaction time. The optimization showed that the highest FAME yield was at 25.1% (w/w) with 10 minutes reaction time, 5% catalyst and 360:1 of the ratio of the methanol to biomass. Based on Analysis of Variance (ANOVA), the model was found to be significant according to the value of “Prob >F” of 0.0028. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

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“…Yeast oil accumulation is dependent on the C/N molar ratio of the OPM (Sathiyamoorthi et al 2019). A higher sugar concentration in the OPM gives a higher C/N molar ratio and subsequently a higher oil accumulation by oleaginous microorganisms (Ahmad et al 2017). The sugar concentration in the OPEFB hydrolysate-based OPM was increased by adding glucose to a final concentration of 50 g/L (OPEFB hydrolysateglucose), whereupon the OPEFB hydrolysate-glucose gave the highest oil yield of N. cerealis IN1S2.5 at 0.8 g/L after 7 d with an increased oil content to 12.45% (g/g DCW), but a decreased cell biomass to 6.4 g/L (Fig.…”

Section: Maximization Of the N Cerealis In1s25 Oil Production In Opefb Hydrolysatementioning

confidence: 99%

Naganishia cerealis IN1S2.5 oil production from the hydrolysate of NaOH-impregnated and catalyst steam explosion pretreated oil palm empty fruit bunch

Weeraphan

Tanasupawat

Savarajara

2021

BioRes

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NaOH-impregnation with catalyst steam explosion was found to be an efficient pretreatment method for oil palm empty fruit bunch (OPEFB) as a substrate for oil production by Naganishia cerealis IN1S2.5. Cellulase hydrolysis of the pretreated OPEFB yielded glucose at 0.364 g/g. Investigation of N. cerealis IN1S2.5 oil production in the OPEFB hydrolysate revealed a maximum oil yield (2.46 g/L) when the C/P molar ratio of the OPEFB hydrolysate was adjusted to 25.71, supplemented with Ca2+ and Zn2+, and set to pH 4. The N. cerealis IN1S2.5 oil was comprised of oleic (37.6%), palmitic (36.2%), and steric (17.9%) acids, all (w/w), as the major fatty acids. Predicted properties of the produced biodiesel indicated the potential of N. cerealis IN1S2.5 oil as a biodiesel feedstock.

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Improved microbial oil production from oil palm empty fruit bunch by Mucor plumbeus

Cited by 24 publications

References 30 publications

Turbo thin film continuous flow production of biodiesel from fungal biomass

Turbo thin film continuous flow production of biodiesel from fungal biomass

Comparison of Ex-Situ and In-Situ Transesterification for the Production of Microbial Biodiesel

Naganishia cerealis IN1S2.5 oil production from the hydrolysate of NaOH-impregnated and catalyst steam explosion pretreated oil palm empty fruit bunch

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