Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

Abstract

The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

An efficient protocol for Cistus crispus L. (Cistaceae) micropropagation

“…The study of the propagation of endangered plants is oriented toward methods, such as micropropagation, that can limit further impoverishment of rare plant material (Fay, 1992;Giovino et al, 2015a;Manokari and Shekhawat, 2018). In conclusion, the protocol used here showed very high performance in number of plants obtained per unit time and could be used for various aims, including its mass production with low costs or the increase in the presence of C. crispus in the natural environment.…”

Cissus antractica -ZnO NPs Induce Apoptosis in A549 Cells through ROS-Mediated p53/Bcl-2/Bax Signaling Pathways and Inhibit Inflammatory Cytokines

“…Cissus Antarctica is a yellowish leaf, lamina ovate to ovateoblong, mostly 4-12 cm long, 20-50 mm wide used traditionally used as a vine in subtropical climates as an ornamental plant in gardens. Cissus species contains saponins, triterpenoids, terpenoids, alcohols, phenols, alkanes, carboxylic acids, alkenes, aliphatic amines and aromatics [34,36,37]. Cissus species has been reported on human health as an antioxidant, antimicrobial, anti-nociceptive, antibacterial, analgesic, anti-inflammatory, antipyretic activity and anti-cancer [38][39][40][41][42] but there is no scientific evidence that Cissus antractica pharmacologically efficacious against lung cancer and inflammation.…”

Response of Turnera subulata cuttings to various types of compound fertilizers and growing medium

“…Cuttings are the most common method of propagation for T. subulata in oil palm plantations. This is due to the fact that seeds of this plant are fairly difficult to obtain, and the rate of growth in large quantities is significantly accelerated by using cuttings [5], [6], [8], [9].…”