Improved micropropagation of Bacopa monnieri (L.) Wettst. (Plantaginaceae) and antimicrobial activity of in vitro and ex vitro raised plants against multidrug-resistant clinical isolates of urinary tract infecting (UTI) and respiratory tract infecting (RTI) bacteria

Haque, Sk Moquammel; Chakraborty, Avijit; Dey, Diganta; Mukherjee, Swapna; Nayak, Sanghamitra; Ghosh, Biswajit

doi:10.1186/s40816-017-0055-6

Cited by 22 publications

(11 citation statements)

References 37 publications

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“…However, our findings confirm all the tissue culture derived ex vitro plants have better antimicrobial activity than naturally propagated plants. These results are in agreed with reports in Bacopa monnieri , Drimia robusta and Cotyledon orbiculata where tissue-culture-derived ex vitro acclimatized plants showed maximum antimicrobial activity [20], [42], [43]. This preliminary findings ensure the bulb of L. revoluta have potential antimicrobial activity .…”

Section: Discussionsupporting

confidence: 92%

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Callus mediated shoot organogenesis and regeneration of cytologically stable plants of Ledebouria revoluta: An ethnomedicinal plant with promising antimicrobial potency

Haque

Chakraborty

Ghosh

2018

Journal of Genetic Engineering and Biotechnology

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Ledebouria revoluta are important ethnomedicinal plant found in India and South Africa. Micropropagation via indirect shoot organogenesis had been established from three types of explant (i.e. scale leaf, leaf lamina and root) of L. revoluta. Scale leaf was found superior as compared to leaf lamina and root explant with respect to their organogenic callus induction potentiality. Murashige and Skoog (1962) [MS] media supplemented with 3.0 mg L−1 2,4-dichlorophenoxyacetic acid, 0.75 mg L−1 β-naphthoxyacetic acid were best effective for inducing organogenic callus. Maximum 17.0 ± 0.52 bulblets were induced from about 500 mg of callus within 42–46 days sub-culturing on a medium containing 0.75 mg L−1 kinetin. The bulblets were matured (86.7% success) after one month culture on the same medium composition. The best result of in vitro root induction with 100% response and 8.4 ± 0.31 roots per bulb was achieved after 18 days of implantation on MS medium containing 2.0 mg L−1 indole-3-butyric acid. Plantlets were acclimatized with a 96.0% survival rate. Chromosomal studies revealed cytological stability of callus cells and all regenerants containing 2n = 30 chromosomes, same as parental plants. Antimicrobial activity of L. revoluta was tested against two Gram-positive bacteria, three Gram-negative bacteria and two fungi. The methanol and ethanol extract proved more effective against bacteria, whereas acetone and chloroform extract shows potential anti-fungal activities. Present protocol can be applied reliably to produce uniform planting materials in large scale. In addition, this efficient indirect regeneration pathway via callus culture opens a way for improvement through genetic transformation.

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Section: Discussionsupporting

confidence: 92%

“…All bacterial strain were tested in Muller Hinton agar medium and fungi were in Saborouds dextrose agar medium. The experiment was carried out following the methods previously described by Haque et al [20]. The dried plant extracts were dissolved in DMSO for dilution and DMSO alone used as negative control.…”

Section: Methodsmentioning

confidence: 99%

Callus mediated shoot organogenesis and regeneration of cytologically stable plants of Ledebouria revoluta: An ethnomedicinal plant with promising antimicrobial potency

Haque

Chakraborty

Ghosh

2018

Journal of Genetic Engineering and Biotechnology

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“…BAP was the best cytokinin in shoot induction and proliferation from different plants and explants, e.g., Bambusa balcooa nodal cuttings [ 49 ], Punica granatum leaf explant [ 50 ], and in plant regeneration from Bauhinia variegate seeds [ 51 ]. In the family Plantaginaceae, BAP was more effective than Kn for shoot multiplication from Bacopa monnieri and Plantago asiatica in vitro cultures [ 52 , 53 ]. A combination of BAP and NAA was more effective in organogenic shoots proliferation of Mondia whitei [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, biotechnological tools, like tissue culture technology, have proven to provide a reliable method for rapid multiplication of the desired plant species and so conserving its germplasm by establishing tissue banks or increasing intervals between subcultures [ 16 , 17 ]. Despite the large number of studies on the in vitro propagation of Plantaginaceae plants [ 18 , 19 ], the application of this in vitro technology to propagate any of the species from genus Anarrhinum is still not fully established.…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling, in vitro propagation, and genetic assessment of the endangered rare plant Anarrhinum pubescens

Abdelsalam

Mahran

Chowdhury

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Anarrhinum pubescens Fresen. (Plantaginaceae) is a rare plant, endemic to the Saint Catherine area, of South Sinai, Egypt. Earlier studies have reported the isolation of cytotoxic and anti-cholinesterase iridoid glucosides from the aerial parts of the plant. The present study aimed to investigate the chemical profiling of the wild plant shoots as well as establish efficient protocols for in vitro plant regeneration and proliferation with further assessment of the genetic stability of the in vitro regenerated plants. Results Twenty-seven metabolites have been identified in wild plant shoots using the Nuclear Magnetic Resonance (NMR) spectroscopy. The metabolites include alkaloids, amino acids, carbohydrates, organic acids, vitamins, and a phenol. In vitro propagation of the plant was carried out through nodal cutting-micropropagation and leaf segment-direct organogenesis. The best results were obtained when nodal cutting explants were cultured on Murashige and Skoog medium with Gamborg B5 vitamins supplemented with 6-benzylaminopurine (BAP) (1.0 mg/L) and naphthaleneacetic acid (NAA) (0.05 mg/L), which gave a shoot formation capacity of 100% and a mean number of shoots of 27.67 ± 1.4/explant. These shoots were successfully rooted and transferred to the greenhouse and the survival rate was 75%. Genetic fidelity evaluation of the micropropagated clones was carried out using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) molecular markers. Jaccard’s similarity coefficient indicated a similarity as high as 98% and 95% from RAPD and ISSR markers, respectively. Conclusions This study provides the chemical profiling of the aerial part of Anarrhinum pubescens. Moreover, in vitro regeneration through different tissue culture techniques has been established for mass propagation of the plant, and the genetic fidelity of the in vitro regenerated plants was confirmed as well. Our work on the in vitro propagation of A. pubescens will be helpful in ex situ conservation and identification of bioactive metabolites.

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“…The promotive role of spermidine in combination with BA in adventitious shoot bud induction from mature leaves excised from in vitro propagated plants was reported couple of years back by [63]. Thus, using explants from tissue culture raised plants direct shoots regeneration leading to production of more than 100 rooted plants/explant within 8-12 weeks period with 85%-100% survival in the field after acclimatization can be expected following optimized protocols summarized in Table 1.…”

Section: Organogenesis From In Vitro Derived Explantsmentioning

confidence: 99%

In Vitro Propagation, Phytochemical and Neuropharmacological Profiles of Bacopa monnieri (L.) Wettst.: A Review

Saha

Sarkar

Jeyasri

et al. 2020

Plants

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Bacopa monnieri has been used as a reputed drug in the Indian traditional ayurvedic system for centuries. This medicinal herb with important phytopharmaceuticals has been popularly known as “Brahmi”. In recent years, B. monnieri has been extensively studied for its bioactive constituents, constituents responsible for memory enhancing effect, and also its diverse other useful effects. It possesses many pharmacological activities such as antioxidant, gastrointestinal, endocrine, antimicrobial, anti-inflammatory etc. The plant has been also used for the treatment of neurological and neuropsychiatric diseases. Due to its multipurpose therapeutic potential, micropropagation using axillary meristems and de novo organogenesis has been extensively studied in the species and is being reviewed. High frequency direct shoot organogenesis can be induced in excised leaf and internode explants in the absence of exogenous phytohormones and the rate of induction is enhanced in the presence of exogenous cytokinins, supplements, growth regulators, etc. Using explants from tissue culture raised plants, direct shoot regeneration leading to production of more than 100 rooted plants/explant within 8–12 weeks period with 85%–100% survival in the field after acclimatization can be expected following optimized protocols. Bioreactor based micropropagation was found to increase the multiplication rate of shoot cultures for the commercial propagation of B. monnieri plants. The maximum content of bacosides has been recorded in shoot biomass using an airlift bioreactor system. Further studies for the biosynthesis of bacosides and other secondary metabolites need to be conducted in the species utilizing untransformed shoot cultures in bioreactors.

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Improved micropropagation of Bacopa monnieri (L.) Wettst. (Plantaginaceae) and antimicrobial activity of in vitro and ex vitro raised plants against multidrug-resistant clinical isolates of urinary tract infecting (UTI) and respiratory tract infecting (RTI) bacteria

Cited by 22 publications

References 37 publications

Callus mediated shoot organogenesis and regeneration of cytologically stable plants of Ledebouria revoluta: An ethnomedicinal plant with promising antimicrobial potency

Callus mediated shoot organogenesis and regeneration of cytologically stable plants of Ledebouria revoluta: An ethnomedicinal plant with promising antimicrobial potency

Metabolic profiling, in vitro propagation, and genetic assessment of the endangered rare plant Anarrhinum pubescens

In Vitro Propagation, Phytochemical and Neuropharmacological Profiles of Bacopa monnieri (L.) Wettst.: A Review

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