Improved penicillin selective enzyme electrode

Cullen, L. F.; Rusling, James F.; Schleifer, Arthur.; Papariello, G. J.

doi:10.1021/ac60349a007

Cited by 64 publications

(12 citation statements)

References 15 publications

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“…We have tested a number of immobilization methods: inorganic bridge formation (17), cyanuric chloride activation (18), adsorption (12), cross-linking (with and without bovine serum albumin) the enzyme into the pad with glutar aldehyde (19), and covalent attachment via silanization of the cellulose, followed by glutar aldehyde coupling of the enzyme to the activated surface (8). Two immobilization methods have proved successful: glutar aldehyde cross-linking of the penicillinase (with or without bovine serum albumin) within the fibrous pad; and covalent attachment via silanization and glutar aldehyde coupling to the cellulose (without bovine serum albumin).…”

Section: Resultsmentioning

confidence: 99%

Flow injection analysis as a diagnostic tool for development and testing of a penicillin sensor

Yerian¹,

Christian²,

Růžička³

1988

Anal. Chem.

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Section: Resultsmentioning

confidence: 99%

Flow injection analysis as a diagnostic tool for development and testing of a penicillin sensor

Yerian¹,

Christian²,

Růžička³

1988

Anal. Chem.

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“…Although the literature is quite replete with studies on β-lactamase-based enzymatic and microbial electrodes for detecting penicillin and its derivatives [17,19,20,[38][39][40], only a few reports concerning cephalosporin detection by such biosensors are available [18,21]. Matasumoto et al [18] constructed a cephalosporinase-based microbial flow-type sensor having Citrobacter freundii that showed good specificity towards cephalosporins but with limited detection range and response time.…”

Section: Substrate Specificity and Interference Due To Other Antibioticsmentioning

confidence: 99%

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Kumar

Kundu

Pakshirajan

et al. 2008

Appl Biochem Biotechnol

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A new potentiometric microbial biosensor based on Pseudomonas aeruginosa was developed in this study for detecting the cephalosporin group of antibiotics. Preliminary results with the biosensor indicated that P. aeruginosa cells, when treated with lysozyme, showed more efficiency in detecting cephalosporin C in a wide concentration range of 0.1-11 mM with high sensitivity compared to the normal cells. Optimization of the three important biosensor design parameters permeabilized cell contents, quantities of gelatin, and glutaraldehyde resulted in high performance of the biosensor. The optimized values of the above parameters were cell contents 2.5 mg/cm(2), gelatin 8.5 mg/cm(2), and 0.25% glutaraldehyde. The assay conditions, namely phosphate buffer pH, ionic strength, and temperature, were optimized for best performance of the biosensor. The specificity test of the biosensor towards detecting different beta-lactam antibiotics showed good response only for the cephalosporins. The operational and storage stability in detecting cephalosporin C indicated very good potential of the biosensor in detecting cephalosporins with high accuracy.

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“…Considerable work has been done with regard to the immobilization of enzymes directly onto or in close proximity to (6)(7)(8)(9)(10) pH, pNH3 glass electrodes or glass-bodied p02 electrodes. However, the response times of most of the enzyme electrodes so far constructed indicate that they normally range from 1 to 5 or even 10 min and that a response time of 25-30 s has been the best achieved.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of thin enzyme membranes to construct glass enzyme electrodes

Kumaran¹,

Meier²,

Danna³

et al. 1991

Anal. Chem.

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International audienceA technique of immobilizing very thin layer of enzymes over glass electrodes, resulting in response times as low as 5-10s, is described. The method involves the adsorption of enzyme molecules onto the sensitive end of the glass electrode followed by the formation of covalent bonds between the enzyme molecules, by means of spraying a dilute solution of glutaraldehyde over the surface. Enzymes immobilized in this manner over pH glass elctrodes are acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), penicillinase, and urease. The cross-sectional scanning electron micrograph of the fractured surface of the electrode shows the immobilized layer is not more than 1-2 µm thick. The technique could offer immense scope for immobilization of other protein substances as thin layers on different support surfaces

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Improved penicillin selective enzyme electrode

Cited by 64 publications

References 15 publications

Flow injection analysis as a diagnostic tool for development and testing of a penicillin sensor

Flow injection analysis as a diagnostic tool for development and testing of a penicillin sensor

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Immobilization of thin enzyme membranes to construct glass enzyme electrodes

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