Improved pharmacological properties for superoxide dismutase modified with mannan

Abstract

Mannan from Sacharomyces cerevisiae was activated by oxidation with NaIO(4) (sodium m-periodate) and further linked to SOD (superoxide dismutase) via reductive alkylation with NaBH(4) (sodium borohydride). The glycosidated enzyme contained an average of 1.2 mol of polysaccharide per mol of protein and retained 52% of its initial activity. The modified enzyme was 560-fold more resistant to inactivation with H(2)O(2) and acquired a lectin-recognition capacity in respect of concanavalin A. The anti-inflammatory activity of SOD was increased 2-fold and its plasma half-life time was prolonged from 4.8 min to 1.7 h after glycosylation with the polymer.

Promotion of Survival and Engraftment of Transplanted Adipose Tissue-Derived Stromal and Vascular Cells by Overexpression of Manganese Superoxide Dismutase

“…The cytoprotective effects of preconditioning can also be achieved by pharmacological treatment, heat shock intervention and gene transfer to promote expression of pro survival genes [ 11 ]. Unfortunately, development of SODs as pharmaceutical products has been hampered by unfavorable pharmacologic and pharmacokinetic properties of the enzymes [ 38 , 39 ]. To counteract SOD rapid renal clearance, gene therapy approaches have been proposed to achieve robust SOD expression and enhanced recovery of tissue injuries has been described [ 40 ].…”