Improved Physiochemical Properties of Chitosan@PCL Nerve Conduits by Natural Molecule Crosslinking

Bianchini, Marta; Zinno, Ciro; Micera, Silvestro; Redolfi Riva, Eugenio

doi:10.3390/biom13121712

Cited by 4 publications

(1 citation statement)

References 69 publications

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“…Among the discussed manufacturing techniques, unidirectional freezing and electrospinning have the highest benefit–cost ratio given that they can mimic the nerve environment with an anisotropic nanofibrillar structure, and tunable porous morphology, which favor nutrient exchange, and subsequently axonal elongation. 246 A wide variety of materials can be processed with these techniques and their relative simplicity and versatility can ensure industrial scalability. Among the reviewed examples, natural-based materials such as COL, chitosan and silk-fibroin represent the best solution for nerve regeneration.…”

Section: Discussionmentioning

confidence: 99%

Beyond the limiting gap length: peripheral nerve regeneration through implantable nerve guidance conduits

Redolfi Riva,

Özkan,

Contreras

et al. 2024

Biomater. Sci.

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Section: Discussionmentioning

confidence: 99%

Beyond the limiting gap length: peripheral nerve regeneration through implantable nerve guidance conduits

Redolfi Riva,

Özkan,

Contreras

et al. 2024

Biomater. Sci.

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Improving Stability and Mechanical Strength of Electrospun Chitosan‐Polycaprolactone Scaffolds Using Genipin Cross‐linking for Biomedical Applications

Uma Thanu Krishnan Neela,

Szewczyk,

Karbowniczek

et al. 2024

Macromol. Rapid Commun.

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Electrospun nanofiber scaffolds have become vital in biomedical applications due to their high surface area and tunable properties. Chitosan (CS) is widely used, but its rapid degradation limits its effectiveness. This study addresses this limitation by blending CS with polycaprolactone (PCL) and applying genipin cross‐linking to enhance its stability and mechanical properties. Scanning electron microscopy indicated a uniform morphology of the electrospun fibers, and further, the crystallinity of the scaffolds before and after cross‐linking is verified. Fourier‐transform infrared spectroscopy is used to analyze the chemical structure, identifying the presence of trifluoroacetic acid residues in the as‐spun fibers. These residues are successfully eliminated through neutralization and cross‐linking, which are critical for enhancing stability and cell viability in in‐vitro studies. Mechanical testing revealed that cross‐linked CS+PCL scaffolds exhibit a 350% increase in tensile strength compared to pure CS, and zeta potential reaches the favorable for cell development ‐26.27 mV. The cytotoxicity assay results with murine NIH 3T3 fibroblast cells indicate the suitability of CS+PCL scaffolds for targeted tissue engineering and wound healing. This work establishes the potential for fine‐tuning scaffold properties to create stable, functional, and biocompatible substrates for extended biomedical use.

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PLCL/SF/NGF nerve conduit loaded with RGD‐TA‐PPY hydrogel promotes regeneration of sciatic nerve defects in rats through PI3K/AKT signalling pathways

Liu,

Tang,

Jin

et al. 2024

J Cellular Molecular Medi

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Peripheral nerve defect are common clinical problem caused by trauma or other diseases, often leading to the loss of sensory and motor function in patients. Autologous nerve transplantation has been the gold standard for repairing peripheral nerve defects, but its clinical application is limited due to insufficient donor tissue. In recent years, the application of tissue engineering methods to synthesize nerve conduits for treating peripheral nerve defect has become a current research focus. This study introduces a novel approach for treating peripheral nerve defects using a tissue‐engineered PLCL/SF/NGF@TA‐PPy‐RGD conduit. The conduit was fabricated by combining electrospun PLCL/SF with an NGF‐loaded conductive TA‐PPy‐RGD gel. The gel, synthesized from RGD‐modified tannic acid (TA) and polypyrrole (PPy), provides growth anchor points for nerve cells. In vitro results showed that this hybrid conduit could enhance PC12 cell proliferation, migration, and reduce apoptosis under oxidative stress. Furthermore, the conduit activated the PI3K/AKT signalling pathway in PC12 cells. In a rat model of sciatic nerve defect, the PLCL/SF/NGF@TA‐PPy‐RGD conduit significantly improved motor function, gastrocnemius muscle function, and myelin sheath axon thickness, comparable to autologous nerve transplantation. It also promoted angiogenesis around the nerve defect. This study suggests that PLCL/SF/NGF@TA‐PPy‐RGD conduits provide a conducive environment for nerve regeneration, offering a new strategy for peripheral nerve defect treatment, this study provided theoretical basis and new strategies for the research and treatment of peripheral nerve defect.

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Improved Physiochemical Properties of Chitosan@PCL Nerve Conduits by Natural Molecule Crosslinking

Cited by 4 publications

References 69 publications

Beyond the limiting gap length: peripheral nerve regeneration through implantable nerve guidance conduits

Beyond the limiting gap length: peripheral nerve regeneration through implantable nerve guidance conduits

Improving Stability and Mechanical Strength of Electrospun Chitosan‐Polycaprolactone Scaffolds Using Genipin Cross‐linking for Biomedical Applications

PLCL/SF/NGF nerve conduit loaded with RGD‐TA‐PPY hydrogel promotes regeneration of sciatic nerve defects in rats through PI3K/AKT signalling pathways

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