Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. igri

A high-resolution transcript linkage map of barley was created using a single doubled haploid (DH) mapping population, only 3 0 -end expressed sequence tags (ESTs) and only PCR-based assays. Cultivar 'Haruna Nijo' and an ancestral wild-form accession 'H602' were used as EST donors and crossing parents of the mapping population. Of the 10 366 primer sets developed from a non-redundant set of 3 0 EST sequences, 7700 sets generated useful amplicons and 3975 (52%) showed polymorphisms between the mapping parents. Of these, 2890 (28% of the total) were mapped by single nucleotide polymorphisms (1717), cleaved amplified polymorphic sequence (933) and INDELs (240). The present work involves an estimated 9% of the genes of barley. Of the mapped ESTs, 2689 (93%) are formatted in the Affymetrix Barley 1 GeneChip and full-length cDNA sequences are available for 1039 (36%). Mapped ESTs show highest similarity with sequences in the wheat gene index (93%) and moderate similarity with rice (50%). Comparison of mapped EST positions and the rice pseudomolecule indicated collinear regions between two species; these are particularly conserved for the entire barley chromosome 3H and rice chromosome 1. These mapped genes, together with a systematically developed set of genetic resources, will make it easier to directly clone genes showing simple inheritance and to determine the genetic basis of complex traits. The information will contribute to the development of a framework for the physical mapping of barley, which is necessary for genome sequencing.

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“…The DH mapping population of 93 lines was derived from the F 1 using microspore culture by Pajbjergfonden, Denmark (Ziauddin et al, 1990).…”

Section: Primer Developmentmentioning

confidence: 99%

A high-density transcript linkage map of barley derived from a single population

2009

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“…Among these, the liquid anther cultures in tobacco (Sunderland and Roberts, 1979), colza (Lichter, 1981), Anemone sp. and Clematis (Johansson et al, 1982), potato (Uhrig, 1985), barley (Ziauddin et al, 1990), and winter triticale (Ponitka and Ślusarkiewicz-Jarzina, 2007) are remarkable. As a type of submethod between anther and microspore culture, the shed-microspore technique has lately drawn attention.…”

Section: Introductionmentioning

confidence: 98%

Androgenic responses of 64 ornamental pepper (Capsicum annuum L.)genotypes to shed-microspore culture in the autumn season

Ari¹,

Bedir²,

Yildirim³

et al. 2016

Turk J Biol

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IntroductionOrnamental peppers (Capsicum annuum L.) have the advantage of multipurpose use in fresh, spice, and pickle consumption, besides being pot and garden plants and cut flower arrangement species. Lately, interest and demand for ornamental peppers has increased. According to Stommel and Bosland (2006), ornamental peppers have begun to regain their previous popularity due to easy seed propagation, relatively short cropping time, and heat/ drought tolerance. The varied foliage color and colorful fruits characterizing ornamental peppers ensure a vivid summer garden display that rivals the chrysanthemum (Dendramthema grandiflora Tzvelev) for brilliant fall color (Stommel and Griesbach, 2005).Steadily growing interest has encouraged intensive breeding studies of ornamental peppers. When evaluated in terms of method, it was seen that the majority of these studies (

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“…In barley, plant regeneration has been achieved in calli from various explant sources: anthers (Jähne et al 1991), microspores (Ziauddin et al 1990), apical meristems (Vitanova et al 1995), immature inflorescences (Thomas and Scott 1985), mature embryos (Ukai and Nishimura 1987), and immature embryos (Rikiishi et al 2003a(Rikiishi et al , 2003b. Immature embryos are currently used as reliable and efficient target tissues for genetic transformation (Cho et al 2002, Scholz et al 2001, Zhang et al 2001.…”

Section: Introductionmentioning

confidence: 99%

Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos

et al. 2008

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Callus growth and shoot regeneration were examined in cultures of immature barley embryos incubated under various combinations of a week under a 16-h photoperiod and a week under continuous darkness. Incubation in darkness for four weeks, during which calli were formed, enhanced shoot regeneration in 'K-3' and 'Kanto Nijo-5', but inhibited it in 'Lenins'. 'K-3' and 'Kanto Nijo-5', incubated in darkness during the first two weeks of callus induction, followed by two weeks of a 16-h photoperiod (D2L2), showed a higher percentage of shoot regeneration than those incubated for two weeks with a 16-h photoperiod followed by two weeks of darkness (L2D2). Nevertheless, when the light conditions, D2L2 and L2D2, were combined into the same periods of a 16-h photoperiod and continuous darkness, except for the order, light conditions affected shoot regeneration differently. The early stage of callus induction seems to be sensitive to light. The expression ratios of the auxin-responsive gene (AUX/IAA) to the cytokinin-responsive gene (WPK4) were increased in continuous darkness in 'K-3' and 'Kanto Nijo-5'. The effects of light qualities (white, red, farred, and blue) on callus growth and shoot regeneration were also examined. Blue light inhibited shoot regeneration, as did white light, in 'K-3' and 'Kanto Nijo-5', and far-red light in 'Kanto Nijo-5'. Light probably controls shoot regeneration from calli by modifying cytokinin levels and/or response; blue light signals act in photo-inhibition of shoot regeneration in immature barley embryo culture.

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Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. igri

Cited by 58 publications

References 9 publications

A high-density transcript linkage map of barley derived from a single population

A high-density transcript linkage map of barley derived from a single population

Androgenic responses of 64 ornamental pepper (Capsicum annuum L.)genotypes to shed-microspore culture in the autumn season

Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos

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