Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

Villers, Agnès; Ris, Laurence

doi:10.3791/50483

Cited by 20 publications

(16 citation statements)

References 42 publications

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“…Next, we tested if loss of DBN affects the continuous process of induction and expression of LTP. Maintenance of LTP is highly dependent on external parameters such as oxygenation and temperature 46 47 . Therefore we tested different conditions that varied the temperature (potentially influencing actin dynamics) and the flow rate of recording solution (potentially affecting oxygenation and/or the washout of secreted factors 48 ), or the composition of the recording solution (addition of glycine and D-serine as co-agonist of NMDA receptors).…”

Section: Resultsmentioning

confidence: 99%

Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin

Willmes

Mack

Ledderose

et al. 2017

Sci Rep

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The dynamic regulation of the actin cytoskeleton plays a key role in controlling the structure and function of synapses. It is vital for activity-dependent modulation of synaptic transmission and long-term changes in synaptic morphology associated with memory consolidation. Several regulators of actin dynamics at the synapse have been identified, of which a salient one is the postsynaptic actin stabilising protein Drebrin (DBN). It has been suggested that DBN modulates neurotransmission and changes in dendritic spine morphology associated with synaptic plasticity. Given that a decrease in DBN levels is correlated with cognitive deficits associated with ageing and dementia, it was hypothesised that DBN protein abundance instructs the integrity and function of synapses. We created a novel DBN deficient mouse line. Analysis of gross brain and neuronal morphology revealed no phenotype in the absence of DBN. Electrophysiological recordings in acute hippocampal slices and primary hippocampal neuronal cultures showed that basal synaptic transmission, and both long-term and homeostatic synaptic plasticity were unchanged, suggesting that loss of DBN is not sufficient in inducing synapse dysfunction. We propose that the overall lack of changes in synaptic function and plasticity in DBN deficient mice may indicate robust compensatory mechanisms that safeguard cytoskeleton dynamics at the synapse.

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Section: Resultsmentioning

confidence: 99%

Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin

Willmes

Mack

Ledderose

et al. 2017

Sci Rep

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“…4) There are several differences in experimental conditions between Yamasaki et al and ours, 4) such as stimulation site and frequency, age of animals, and temperature during recording, which are all known to affect neuronal activity. [10][11][12] Also, as shown in Figs. 2A and B, the amplitude of fEPSP depends on stimulation intensity, yet Yamasaki et al did not mention how much intensity they gave.…”

Section: Resultsmentioning

confidence: 85%

Ectopic Mossy Fiber Pathfinding in the Hippocampus Caused the Abnormal Neuronal Transmission in the Mouse Models of Psychiatric Disease

Nakahara

Matsumoto

Ito³

et al. 2018

Biological & Pharmaceutical Bulletin

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Appropriate axonal pathfinding is a necessary step for the function of neuronal circuits. The mossy fibers (MFs) in the hippocampus of CaMKIIα heterozygous knockout (CaMKIIα-hKO) psychiatric model mice project onto not only the stratum lucidum but also the stratum oriens region in the CA3, which is a projection pattern distinct from that in normal mice. Thus, we examined the electrophysiological properties of the MF-CA3 connection in this mutant mouse on field recordings and found a lower synaptic connection. This study suggested that the phenotype of abnormal MF pathfindings could induce aberrant neuronal functions, which may link to cognition and memory.Key words mossy fiber; immature dentate gyrus; field recording; infrapyramidal bundle; stratum oriens; psychiatry Therapeutic interventions for psychiatric disorders such as schizophrenia (SCZ) are hampered by a lack of clear molecular signatures underlying the pathophysiology of the disorders. However, several studies point to abnormal neuronal connectivity in the hippocampus, which potentially relates to the cognitive impairments observed in SCZ patients.1) The abnormal hippocampal synaptic transmission found in SCZ patients could result from inappropriate neuronal connectivity.Appropriate axonal guidance has to be established during development and maintained throughout life to coordinate neural circuits and their functions. Mossy fibers (MFs), which are axons of granule cells in the dentate gyrus (DG), innervate to the CA3 subregion, forming a hippocampal circuit between DG and CA3. During postnatal development, MFs extend to both the stratum lucidum (SL) and the stratum oriens (SO), forming supra-pyramidal bundles (SPB) and infra-pyramidal bundles (IPB), respectively. The formation of IPB, however, is transient and undergoes pruning during development, leaving mostly SPB upon maturation.2) It is of note that some pathological conditions such as temporal lobe epilepsy result in ectopic formation of IPB.3) The CaMKIIα heterozygous knockout (CaMKIIα-hKO) mouse is considered one of the psychiatric model mice as they showed hyper locomotor activity, the deficits of working memory and social interaction, 4,5) which also have the ectopic MF projection in SO. 6) In the present study, we investigated the electrophysiological properties in the MF-CA3 connection in CaMKIIα-hKO mice to characterize the functional abnormalities of the ectopic MF distribution. MATERIALS AND METHODSTwo-to four-month-old male mice (age-matched littermates) were used for the experiments. CaMKIIα-hKO mice were generated and maintained in a C57BL6/N tac background 7)

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“…Even though classical demonstration of LTP induction is demonstrated in the hippocampal CA3-CA1 region, LTP has been demonstrated in various locations in the nervous system. When a stimulating electrode stimulates large number of CA3 neuronal axons (Schaffer collaterals) ( Figure 5(a)) whose terminals synapse with dendritic spines of the neurons of the CA1 layer, a recording electrode placed close to the main dendritic stems of the CA1 neurons records electrical changes [41]. The electrical settings of the input electrode are adjusted until the output neuron fires 50% of the time (labeled as "amplitude of 50%").…”

Section: Interpostsynaptic Functional Link and Ltpmentioning

confidence: 99%

An Electronic Circuit Model of the Interpostsynaptic Functional LINK Designed to Study the Formation of Internal Sensations in the Nervous System

Vadakkan

2014

Advances in Artificial Neural Systems

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The nervous system makes changes in response to the continuous arrival of associative learning stimuli from the environment and executes behavioral motor activities after making predictions based on past experience. The system exhibits dynamic plasticity changes that involve the formation of the first-person internal sensations of perception, memory, and consciousness to which only the owner of the nervous system has access. These properties of natural intelligence need to be verified for their mechanism of formation using engineered systems so that a third person can access them. In the presence of a synaptic junctional delay of up to two milliseconds, we anticipate that the systems property of formation of internal sensations is likely independent of the mode of conduction along the neuronal processes. This allows testing for the formation of internal sensations using electronic circuits. The present work describes the neurobiological context for the formation of the basic units of inner sensations that occur through the reactivation of interpostsynaptic functional LINKs and its connection to motor activity. These mechanisms are translated to an analogue circuit unit for the development of robotic systems.

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Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

Cited by 20 publications

References 42 publications

Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin

Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin

Ectopic Mossy Fiber Pathfinding in the Hippocampus Caused the Abnormal Neuronal Transmission in the Mouse Models of Psychiatric Disease

An Electronic Circuit Model of the Interpostsynaptic Functional LINK Designed to Study the Formation of Internal Sensations in the Nervous System

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