Improved preservation of human red blood cells by lyophilization

Frozen Red Blood Cells in Transfusion

“…Lyophilised RBC is envisaged to offer many advantages such as weight, convenient transportation, room temperature preservation and ease of reconstitution [19]. However 'Lyophilised RBC' is in experimental stages and has long way for clinical use [19]. Thus FS-RBC is the only option in augmenting current liquid blood supply for Armed Forces Blood Programme.…”

“…Various groups are working on lypholisation as modality in storage of RBC. Lyophilised RBC is envisaged to offer many advantages such as weight, convenient transportation, room temperature preservation and ease of reconstitution [19]. However 'Lyophilised RBC' is in experimental stages and has long way for clinical use [19].…”

“…Currently various groups are pursuing research on artificial blood and various modalities for storage of RBC [18,19]. An oxygen carrier molecule as substitute for RBC transfusion is under research and may reshape the practice of transfusion medicine [18].…”

Synergistic Effect of Trehalose and Saccharose Pretreatment on Maintenance of Lyophilized Human Red Blood Cell Quality

“…Hemoglobin, the main RBC protein, has an important role in carrying oxygen and carbon dioxide [17] and is an NO storage library; hemoglobin is an antioxidant and participates in homeostasis and platelet aggregation [18]. Therefore, the RBC counts and total hemoglobin recovery rates are two acceptable indices used to evaluate the effects of trehalose and saccharose pretreatment on maintenance of lyophilized RBC quality [19]. Trehalose and saccharose pretreatment synergistically decreased lyophilizationrehydration-induced damage on RBC counts and total hemoglobin by increasing the RBC counts and total hemoglobin recovery rates (Fig.…”

Freeze-drying as an alternative method of human sclera preservation

“…This alternative technique has been used to preserve many biological products such as bovine pericardium (9,11) , human red blood cells (12) , epidermal keratinocytes (13) , amniotic membrane (14) , vaccines (15) and others.…”