Improved processing of secretory proteins in Hansenula polymorpha by sequence variation near the processing site of the alpha mating factor prepro sequence

Eilert, Eva; Rolf, Theresa; Heumaier, Andreas; Hollenberg, Cornelis P.; Piontek, Michael; Suckow, Manfred

doi:10.1016/j.jbiotec.2012.08.024

Cited by 6 publications

(1 citation statement)

References 30 publications

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“…Secretion can be further enhanced through optimization of the residues at the cleavage point between the signal sequence and the protein of interest (Eilert et al., 2013; Wetzel et al., 2016; Yang et al., 2013; Zheng et al., 2016). In Ko.…”

Section: Expression Hostsmentioning

confidence: 99%

Precision cellular agriculture: The future role of recombinantly expressed protein as food

Dupuis

Cheung

Newman

et al. 2022

Comp Rev Food Sci Food Safe

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Cellular agriculture is a rapidly emerging field, within which cultured meat has attracted the majority of media attention in recent years. An equally promising area of cellular agriculture, and one that has produced far more actual food ingredients that have been incorporated into commercially available products, is the use of cellular hosts to produce soluble proteins, herein referred to as precision cellular agriculture (PCAg). In PCAg, specific animal‐ or plant‐sourced proteins are expressed recombinantly in unicellular hosts—the majority of which are yeast—and harvested for food use. The numerous advantages of PCAg over traditional agriculture, including a smaller carbon footprint and more consistent products, have led to extensive research on its utility. This review is the first to survey proteins currently being expressed using PCAg for food purposes. A growing number of viable expression hosts and recent advances for increased protein yields and process optimization have led to its application for producing milk, egg, and muscle proteins; plant hemoglobin; sweet‐tasting plant proteins; and ice‐binding proteins. Current knowledge gaps present research opportunities for optimizing expression hosts, tailoring posttranslational modifications, and expanding the scope of proteins produced. Considerations for the expansion of PCAg and its implications on food regulation, society, ethics, and the environment are also discussed. Considering the current trajectory of PCAg, food proteins from any biological source can likely be expressed recombinantly and used as purified food ingredients to create novel and tailored food products.

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