Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium sp. and Thraustochytriidae sp. through bioprocess optimization

Wang, Qiuzhen; Ye, Huike; Sen, Biswarup; Xie, Yunxuan; He, Yaodong; Park, Sunghoon; Wang, Guangyi

doi:10.1016/j.synbio.2018.04.001

Cited by 41 publications

(28 citation statements)

References 38 publications

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“…Furthermore, a combination of peptone and yeast extract showed the highest potential for DHA production (0.11 g/g, 0.77 g/L), probably because of the presence of amino acids and vitamins which are known to promote cell growth and DHA accumulation. These findings are consistent with previous studies which reported the better action of organic than inorganic nitrogen sources on lipid production [33,34,54]. Our results provide evidence that a combined organic nitrogen source can significantly improve DHA production over a single nitrogen source.…”

Section: Discussionsupporting

confidence: 93%

“…Our results provide evidence that a combined organic nitrogen source can significantly improve DHA production over a single nitrogen source. In addition to carbon and nitrogen sources, temperature, initial pH and rotation rate are also known to exhibit a significant effect on DHA production, and our results are consistent with the earlier findings [33,54]. However, consistent with a previous report [33], we did not observe a significant effect of KH 2 PO 4 and salinity on DHA production by the strain ZJWZ-7 in the present study.…”

Section: Discussionsupporting

confidence: 93%

“…On the contrary, the group thraustochytrids has gained significant attention of researchers because of their biotechnological potential [28]. Especially, some species of Schizochytrium and Aurantiochytrium exhibit the ability to produce large amounts of polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) used in dietary supplements [13,24,25,29,30,31,32,33]. Besides DHA, some strains of thraustochytrids (e.g., Schizochytrium sp.…”

Section: Introductionmentioning

confidence: 99%

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Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China

Wang

Xie

et al. 2019

Marine Drugs

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Labyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, their lipid profiles are poorly described because only a few large-scale isolation attempts have been made so far. Here, we isolated more than 200 strains from mangrove habitats of China and characterized the molecular phylogeny and lipid accumulation potential of 71 strains. These strains were the closest relatives of six genera namely Aurantiochytrium, Botryochytrium, Parietichytrium, Schizochytrium, Thraustochytrium, and Labyrinthula. Docosahexaenoic acid (DHA) production of the top 15 strains ranged from 0.23 g/L to 1.14 g/L. Two labyrinthulid strains, GXBH-107 and GXBH-215, exhibited unprecedented high DHA production potential with content >10% of biomass. Among all strains, ZJWZ-7, identified as an Aurantiochytrium strain, exhibited the highest DHA production. Further optimization of culture conditions for strain ZJWZ-7 showed improved lipid production (1.66 g/L DHA and 1.68 g/L saturated fatty acids (SFAs)) with glycerol-malic-acid, peptone-yeast-extract, initial pH 7, 28 °C, and rotation rate 150 rpm. Besides, nitrogen source, initial pH, temperature, and rotation rate had significant effects on the cell biomass, DHA, and SFAs production. This study provides the identification and characterization of nearly six dozen thraustochytrids and labyrinthulids with high potential for lipid accumulation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China

Wang

Xie

et al. 2019

Marine Drugs

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“…and Schizochytrium spp. (Wang et al, 2018). Moreover, microalgae with high nutritional values are widely used in aquaculture, either for direct consumption for molluscs and shrimp, or indirectly through live feed for fish larvae (Reitan et al, 1997;Brown and Robert, 2002).…”

Section: Introductionmentioning

confidence: 99%

Influence of Nitrogen Limitation on Lipid Accumulation and EPA and DHA Content in Four Marine Microalgae for Possible Use in Aquafeed

Wang

Fosse

et al. 2019

Front. Mar. Sci.

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Microalgae are regarded as a promising alternative that can replace fishmeal and fish oil in aquaculture. Under N-limitation, many microalgae species change their carbon storage patterns in favor of neutral lipids (NLs) mainly in the form of triacylglycerol (TAG), but fatty acids in polar lipids (PL) are nutritionally more available for fish than those esterified into NLs. In the present study, the effect of N-limitation on the lipid content and fatty acid profiles in different lipid classes of Phaeodactylum tricornutum, Isochrysis aff. galbana clone T-Iso, Rhodomonas baltica, and Nannochloropsis oceanica were investigated. The microalgae cells were cultivated by two different methods, batch and semi-continuous culture, to create strong and moderate N-limitation, and this in turn will significantly affect the biomass and lipid productivity. All four species accumulated lipids mainly in the form of TAG, in response to strong nitrogen limitation. N. oceanica, however, accumulated 51% of the dry weight as lipid in moderate nitrogen limitation and up to 87% of the fatty acid was in TAG. Isochrysis aff. galbana clone T-Iso was the only species where the fraction of polyunsaturated fatty acid (PUFA), especially the fraction of docosahexaenoic acid (DHA), increased with increasing nitrogen limitation. Total lipid productivity showed no increase in batch culture although stronger nitrogen limitation led to lipid accumulation. P. tricornutum had the highest eicosapentaenoic acid (EPA) content, while N. oceanica showed the highest EPA productivity due to the high content of lipid. The highest DHA productivity was found in Isochrysis aff. galbana clone T-Iso from moderate N-limitation, mainly due to the high biomass productivity. Based on the results from the current study, N. oceanica and T-Iso are two promising microalgae strains ass long-term sustainable sources of n-3 long chain-PUFAs under moderate N-limitation. As shown in the present study, increased lipid content in microalgal cells due to strong N-limitation induction may not increase the lipid productivity because biomass production is usually reduced. Therefore, a combination of approaches such as metabolic engineering, conditioning and selection may be needed to further increase the n-3 LC-PUFA productivity without substantial loss of biomass.

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“…Maximum DHA yield (Y p/x ) of 21.0% and 18.9% and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained, respectively, in a 5 L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy in Schizochytrium sp. [19]. Nevertheless, it is difficult for the wild-type (WT) strain to meet the requirements of industrial production due to the low biomass and n-3 LC-PUFA content, which accounts for the high cost of the downstream process [20].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

Liu

et al. 2020

Microorganisms

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Docosahexaenoic acid (DHA), a n-3 long-chain polyunsaturated fatty acid, is critical for physiological activities of the human body. Marine eukaryote Aurantiochytrium sp. is considered a promising source for DHA production. Mutational studies have shown that ultraviolet (UV) irradiation (50 W, 30 s) could be utilized as a breeding strategy for obtaining high-yield DHA-producing Aurantiochytrium sp. After UV irradiation (50 W, 30 s), the mutant strain X2 which shows enhanced lipid (1.79-fold, 1417.37 mg/L) and DHA (1.90-fold, 624.93 mg/L) production, was selected from the wild Aurantiochytrium sp. Instead of eicosapentaenoic acid (EPA), 9.07% of docosapentaenoic acid (DPA) was observed in the mutant strain X2. The comparative transcriptomic analysis showed that in both wild type and mutant strain, the fatty acid synthesis (FAS) pathway was incomplete with key desaturases, but genes related to the polyketide synthase (PKS) pathway were observed. Results presented that mRNA expression levels of CoAT, AT, ER, DH, and MT down-regulated in wild type but up-regulated in mutant strain X2, corresponding to the increased intercellular DHA accumulation. These findings indicated that CoAT, AT, ER, DH, and MT can be exploited for high DHA yields in Aurantiochytrium.

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Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium sp. and Thraustochytriidae sp. through bioprocess optimization

Cited by 41 publications

References 38 publications

Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China

Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China

Influence of Nitrogen Limitation on Lipid Accumulation and EPA and DHA Content in Four Marine Microalgae for Possible Use in Aquafeed

Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

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