Improved Production of High-Quality Potato Seeds in Aeroponics with Plant-Growth-Promoting Rhizobacteria

Tkachenko, Oksana V.; Evseeva, Nina V.; Terentyeva, E. V.; Burygin, Gennady L.; Shirokov, Alexander; Буров, А. М.; Matora, L. Yu.; Shchyogolev, S. Yu.

doi:10.1007/s11540-020-09464-y

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…Our previous studies have shown that the associative rhizospheric bacteria A. baldaniorum Sp245 (Tkachenko et al, 2015) and O. cytisi IPA7.2 (Burygin et al, 2019) can be used to promote the growth of potato microplants in vitro and ex vitro. The ability of Azospirillum bacteria to promote potato growth and productivity, including in the seed production system, is well known (Naqqash et al, 2016;Kargapolova et al, 2020;Tkachenko et al, 2021). The efficacy of use of these bacteria is higher in vitro (optimal conditions) but is lower when plants are grown in the field (Bacilio et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2

et al. 2022

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Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material.

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Section: Discussionmentioning

confidence: 99%

Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2

et al. 2022

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“…with their biochemical activity and in particular with the increase of indole-3-acetic acid content (Kargapolova et al 2020). Tkachenko et al (2020) reported that under aeroponic conditions, A. brasilense Sp245 inoculations increased the number of minitubers in varieties Kondor (+40%) and Nevsky (+60%) leading to an average productive increase of 17% in comparison with the uninoculated control. Regarding tuber quality, inoculations increased the starch content in minitubers (by 1.5 times in comparison with the uninoculated control).…”

Section: Microbial Plant Biostimulantmentioning

confidence: 99%

Plant Biostimulants in Sustainable Potato Production: an Overview

Caradonia

Ronga

Tava³

et al. 2021

Potato Res.

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In modern agriculture, the reduction of environmental impact, ensuring both high and stable yield and high quality, is a primary goal. To avoid an excessive use of external inputs, without compromising crop performance, the increase of soil nutrient availability and nutrient use efficiency is fundamental in a sustainable potato production. In this framework, the use of plant biostimulants, such as humic substances, seaweed and microalgal extracts, hydrolysed proteins and amino acids and microorganisms, could give a great help to farmers. Due to the agronomic importance and the keen interest in these substances, the aim of this review was to survey and summarise the outputs derived from scientific published studies on the combination of potato and plant biostimulants. In the literature, several in vitro, growth chamber, glasshouse and field experiments were reported. Field experiments were conducted in different environmental conditions, using different genotypes, and all the applied plant biostimulants showed beneficial effects on both productive and qualitative potato parameters. Seaweed extract and humic substances could also reduce the normal dose of fertiliser without reducing yield. Among quality features, tuber size, contents of protein, vitamin C, starch and phenols are the principal parameters influenced by plant biostimulants. Weaknesses related to the use of plant biostimulants were shown and were related to different effectiveness of products due to their origin, production process and microbial species. In addition, product/strain-genotype interactions have been shown. Environmental factor, agricultural practice and timing of treatment can influence the effectiveness of both microbial and non-microbial plant biostimulants.

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“…At the same time, from the point of view of increasing the stability of the plant-microbial association, inoculation of plants by a consortium of strains is preferable [47]. Data on the growth-stimulating activity of single A. baldaniorum Sp245 in aeroponics coincide with the established effect of co-inoculation by two strains [9]. In contrast to our previous studies, this work establishes mechanisms for increasing the growth activity and productivity of plants through the regulation of the pro/antioxidant activity of the system by rhizobacteria.…”

Section: Discussionmentioning

confidence: 91%

“…Thirty-day-old microplants were planted in an aeroponic system (Figure 1). The plants were grown under a 16-h day and 8-h night photoperiod at a daytime temperature of 25 • C and a night-time temperature of 20 • C. The composition of the plant growth nutrient solution was described by Tkachenko et al [9]. After 2 weeks of growth, the concentration of the main nutrient solution was doubled.…”

Section: Experimental Planmentioning

confidence: 99%

Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

et al. 2023

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Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potato microclones adapting to ex vitro growth in an aeroponic system. Potato (Solanum tuberosum L. cv. Nevsky) microplants were inoculated in vitro with the bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2. On days 1 and 7 of plant growth ex vitro, catalase and peroxidase activities in the leaves of inoculated plants were 1.5-fold higher than they were in non-inoculated plants. The activity of ascorbate peroxidase was reduced in both in vitro and ex vitro treatments, and this reduction was accompanied by a decrease in the leaf content of hydrogen peroxide and malondialdehyde. As a result, inoculation contributed to the regulation of the plant pro/antioxidant system, lowering the oxidative stress and leading to better plant survival ex vitro. This was evidenced by the higher values of measured morphological and physiological variables of the inoculated plants, as compared with the values in the control treatment. Thus, we have shown some PGPR-mediated mechanisms of potato plant protection from adverse environmental factors under aeroponic conditions.

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Improved Production of High-Quality Potato Seeds in Aeroponics with Plant-Growth-Promoting Rhizobacteria

Cited by 15 publications

References 23 publications

Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2

Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2

Plant Biostimulants in Sustainable Potato Production: an Overview

Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

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