Improved production of tannase by Klebsiella pneumoniae using Indian gooseberry leaves under submerged fermentation using Taguchi approach

Abstract

Tannase (tannin acyl hydrolase E.C 3.1.1.20) is an inducible, largely extracellular enzyme that causes the hydrolysis of ester and depside bonds present in various substrates. Large scale industrial application of this enzyme is very limited owing to its high production costs. In the present study, cost effective production of tannase by Klebsiella pneumoniae KP715242 was studied under submerged fermentation using different tannin rich agro-residues like Indian gooseberry leaves (Phyllanthus emblica), Black plum leaves (Syzygium cumini), Eucalyptus leaves (Eucalyptus glogus) and Babul leaves (Acacia nilotica). Among all agro-residues, Indian gooseberry leaves were found to be the best substrate for tannase production under submerged fermentation. Sequential optimization approach using Taguchi orthogonal array screening and response surface methodology was adopted to optimize the fermentation variables in order to enhance the enzyme production. Eleven medium components were screened primarily by Taguchi orthogonal array design to identify the most contributing factors towards the enzyme production. The four most significant contributing variables affecting tannase production were found to be pH (23.62 %), tannin extract (20.70 %), temperature (20.33 %) and incubation time (14.99 %). These factors were further optimized with central composite design using response surface methodology. Maximum tannase production was observed at 5.52 pH, 39.72 °C temperature, 91.82 h of incubation time and 2.17 % tannin content. The enzyme activity was enhanced by 1.26 fold under these optimized conditions. The present study emphasizes the use of agro-residues as a potential substrate with an aim to lower down the input costs for tannase production so that the enzyme could be used proficiently for commercial purposes.

Untitled

“…[5] The basic principle of this method serves as screening filters that examine the effects of many process variables and identify those factors that have major effects on the process. [6] However, it is difficult to determine the key factors of the fermentation medium and to optimize the cultural conditions with single dimensional traditional methods as they are laborious, time consuming, and incapable of reaching the true optimal point due to the interaction between the variables. The response surface method (RSM) is one of the popularly used optimization procedures mainly developed based on full factorial central composite design (CCD).…”

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

“…3.1.1.20), commonly known as tannase is an important hydrolytic enzyme belongs to the esterase super family. 1 It is an attractive biocatalyst and an inducible enzyme which is widely utilized for tannin degradation. It helps in the hydrolysis of the ester bond (galloyl ester of alcohol moiety) and depside bond (galloyl ester of gallic acid) present in hydrolysable tannins thereby releasing simple phenolic molecules like gallic acid and some gallic acid esters along with glucose molecule.…”

Screening and Optimization of Culture Conditions for Local Production of Tannase from Fungal Species

“…3.1.1.20) catalyzes the hydrolysis of ester bonds present in hydrolysable tannins such as gallotannins, ellagitannins, and gallic acid esters resulting in the production of gallic acid and glucose. 1 Tannase is produced extensively from microorganisms as they are very diverse naturally and their environmental and genetic manipulation is easier. The use of microorganism gives numerous advantages including generation of novel producers in reduced time period or reduced generation time.…”