Improved quality control method for Fufang Danshen preparations through simultaneous determination of phenolic acids, saponins and diterpenoid quinones by HPLC coupled with diode array and evaporative light scattering detectors

Wei, Yingjie; Qi, Lian‐Wen; Li, Ping; Luo, Hou-Wei; Liu, Yi; Sheng, Liang-Hong

doi:10.1016/j.jpba.2007.07.013

Cited by 67 publications

(33 citation statements)

References 22 publications

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“…The establishment of good quality control methods is of urgent importance for medical applications. Generally, the therapeutic effects of Chinese medicine preparations are integrative results of multiple bioactive compounds (Wei et al, 2007). The protoberberine alkaloids, such as berberine, palmatine, jatrorrhizine, and epiberberine are found in Coptidis Rhizoma, and indolequinazoline alkaloids, such as evodiamine and rutaecarpine are found in Euodiae Fructus (structures are shown in Figure 1) and are considered to be biologically active based on pharmacological studies (Kong et al, 2009b;Yan et al, 2007;Yang et al, 2009;Yarosh et al, 2006;Yu et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

Gào

Yang

Marriott

2013

Pharmaceutical Biology

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Context: Coptidis Rhizoma-Euodiae Fructus couple (CEC) is a classic traditional Chinese medicine preparation consisting of Coptidis Rhizoma and Euodiae Fructus at the ratio of 6:1, and used to treat gastro-intestinal disorders. Alkaloids are the main bioactive component. This research provides comprehensive analysis information for the quality control of CEC. Objective: To develop a high-performance liquid chromatography-diode array detection fingerprint for chemical composition characteristics of CEC and its products. Materials and methods: The samples were separated with a Gemini C 18 column by using gradient elution with water-formic acid (100:0.03) and acetonitrile as mobile phase. Flow rate was 1.0 mL/ min and detection wavelength was 250 nm. Similarity analysis and principal component analysis (PCA) were employed to evaluate quality consistencies of analytes. Mean chromatograms and correlation coefficients of analytes were calculated by the software ''Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine''. Results: Fingerprint chromatogram comparison determined 20 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.988. Consistent results were obtained to show that CEC and its related samples could be successfully divided into three groups. Contribution plots generated by PCA were performed to interpret differences among the sample groups while peaks which significantly contributed to classification were identified. Seven bioactive constituents in the samples were verified by quantitative analysis. Discussion and conclusion: The chromatographic fingerprint with similarity evaluation and PCA assay combined with quantification of seven compounds could be utilized as a quality control method for the herbal couple.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

Gào

Yang

Marriott

2013

Pharmaceutical Biology

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“…1 It mainly exists in the tropical and subtropical zones of China, including Guangdong, Guangxi, Yunnan, and Fujian. The fruit of the Brucea javanica plant is characteristically ovate in shape, hard, and slightly apiculate at the apex with an approximate length of 6-10 mm and diameter of 4-7 mm.…”

Section: Introductionmentioning

confidence: 99%

Chemical components, pharmacological properties, and nanoparticulate delivery systems of Brucea javanica

Chen

Chen²,

Wang³

et al. 2013

IJN

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Brucea javanica has demonstrated a variety of antitumoral, antimalarial, and anti-inflammatory properties. As a Chinese herbal medicine, Brucea javanica is mainly used in the treatment of lung and gastrointestinal cancers. Pharmacological research has identified the main antitumor components are tetracyclic triterpene quassinoids. However, most of these active components have poor water solubility and low bioavailability, which greatly limit their clinical application. Nanoparticulate delivery systems are urgently needed to improve the bioavailability of Brucea javanica. This paper mainly focuses on the chemical components in Brucea javanica and its pharmacological properties and nanoparticulate formulations, in an attempt to encourage further research on its active components and nanoparticulate drug delivery systems to expand its clinical applications. It is expected to improve the level of pharmaceutical research and provide a strong scientific foundation for further study on the medicinal properties of this plant.

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“…In recent years, an on-line coupled HPLC-DAD-ELSD method has attracted ever-increasing attention and has been successfully applied to simultaneous quantification of multi-components in traditional Chinese herbal medicines and their preparations [14][15][16][17][18][19][20][21][22][23]. This method is potentially ideal for routine analysis and quality evaluation of YST since UV and ELSD signals contain complementary information for each other.…”

Section: Introductionmentioning

confidence: 99%

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu¹,

Jia²,

Cai³

et al. 2013

Acta Chromatographica

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Summary. Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min −1 . The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

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Improved quality control method for Fufang Danshen preparations through simultaneous determination of phenolic acids, saponins and diterpenoid quinones by HPLC coupled with diode array and evaporative light scattering detectors

Cited by 67 publications

References 22 publications

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

Chemical components, pharmacological properties, and nanoparticulate delivery systems of Brucea javanica

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

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