Improved Release of Monosaccharides and Ferulic Acid Using Enzyme Blends From Aspergillus Niger and Eupenicillium Parvum

Liu, Zhenghui; Shi, Enze; Feng, Mang; Zhou, Xin; Jiang, Kankan

doi:10.3389/fbioe.2021.814246

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…The CMCase activity, xylanase activity, and FAE activity of FA12 were measured on the third day of five consecutive passages. The kinds of phenolic acids released by the microbial consortium and the amount of FA release were studied by LC–MS qualitative and quantitative analysis . At the same time, the weight loss rate and changes of four components (cellulose, hemicellulose, acid-insoluble lignin, and acid-soluble lignin) during the microbial treatment were determined.…”

Section: Methodsmentioning

confidence: 99%

“…The kinds of phenolic acids released by the microbial consortium and the amount of FA release were studied by LC−MS qualitative and quantitative analysis. 22 At the same time, the weight loss rate and changes of four components (cellulose, hemicellulose, acid-insoluble lignin, and acidsoluble lignin) during the microbial treatment were determined. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) were used to study the change in the structure characteristics of the distiller's grains before and after microbial treatment.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Fermenting Distiller’s Grains by the Domesticated Microbial Consortium To Release Ferulic Acid

Zhang,

Ye,

Liu

et al. 2024

J. Agric. Food Chem.

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The microbial consortium FA12 that can release ferulic acid (FA) by fermenting distiller's grains was screened from Daqu. Taibaiella, Comamonadaceae, and Ochrobacum were highly abundant in FA12 by 16S rRNA gene sequencing. In the process of long-term acclimation with distiller's grains as a medium, the biomass of FA12 remained stable, and the pH value of fermentation liquid was also relatively stable. Meanwhile, the activities of cellulase, xylanase, and feruloyl esterase secreted by FA12 were stable in the ranges of 0.2350−0.4470, 0.1917−0.3078, and 0.1103−0.1595 U/mL, respectively, and the release of FA could reach 133.77 μg/ g. It is proven that the microbial consortium has good genetic stability. In addition, the structural changes of lignocellulose in distiller's grains before and after fermentation were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR), and the changes of distiller's grains weight and lignocellulose content before and after fermentation were also detected. These results all confirmed that FA12 had the function of degrading distiller's grains. In this study, we explored a method to use microbial communities to release FA from distiller's grains and degrade lignocellulose in the waste, which opened up a new way for the application of the high value of lost waste.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Fermenting Distiller’s Grains by the Domesticated Microbial Consortium To Release Ferulic Acid

Zhang,

Ye,

Liu

et al. 2024

J. Agric. Food Chem.

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“…This compound combination of specific depolymerizing carbohydrases, also called zymogram, synergistically acts on AX, aiding in the release of monosaccharides and the improvement of the degradation rate [ 9 , 10 ]. Therefore, EA removes the arabinose residues in order to expose more cleavage sites and enhance EX accessibility [ 11 , 12 ], while EF cleaves ferulic acid ester bonds cross-linked to arabinose residues in order to liberate ferulic acid [ 13 , 14 ]. Accordingly, our previous work systematically demonstrated that water-extractable AX and water-unextractable AX were hydrolyzed more effectively in vitro by the special xylanase collaborations with EA and EF [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Compound Ratio of Exogenous Xylanase and Debranching Enzymes Supplemented in Corn-Based Broiler Diets Using In Vitro Simulated Gastrointestinal Digestion and Response Surface Methodology

Zhou

Chen

et al. 2022

Animals

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This experiment aimed to explore the zymogram of endo-xylanase (EX) and debranching enzymes (arabinofuranosidase [EA] and ferulic acid esterase [EF]) supplemented in the corn–soybean meal-based diet of broilers. An in vitro simulated gastrointestinal digestion model was adopted. According to single-factor, completely random design, the optimal supplemental levels of individual carbohydrase were determined by reducing sugars (RS) and in vitro dry matter digestibility (IVDMD). Response surface method (RSM) was used to predict the proper compound ratio of three carbohydrases. Results showed that shifts were different for feedstuffs such as corn–soybean meal–distillers dried grains with solubles, corn hull, and wheat bran, revealing that the net increase of RS or IVDMD distinctly dropped when degrading corn and related by-products by EX (p < 0.05). There was a significant quadratic relationship between the above response metrics and addition levels of each enzyme (p < 0.05). The determined dosage was 54 U/g EX, 5.0 U/g EA, and 0.4 U/g of EF, respectively. The optimistic zymogram of carbohydrases in corn basal substrates was judged by the IVDMD screening (R2 = 0.9089, p < 0.001). Conclusively, the in vitro assay and RSM were convenient and rapid methods for the optimization of xylan-degrading zymogram, and also testified asthenic hydrolysis of corn arabinoxylan by EX, thus highlighting the synergistic combinations with debranching enzymes.

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Synergistically Enhanced Enzymatic Hydrolysis of Sugarcane Bagasse Mediated by a Recombinant Endo-Xylanase from Streptomyces ipomoeae

Li,

Dong,

Liu

et al. 2024

Processes

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Xylanase is commonly thought to effectively cooperate with cellulase to promote the bioconversion of lignocellulose. In this study, a novel xylanase, SipoEnXyn10A (Xyn10A), previously identified from Streptomyces ipomoeae, was employed to investigate its synergetic effects on sugarcane bagasse (SCB) transformation. It was shown that the relative increase in reducing sugars reached up to 65%, with enhanced yields of glucose and xylose by 78% and 50%, respectively, in the case of the replacement of cellulase with an equivalent amount of Xyn10A at an enzyme loading of 12.5%. The highest degrees of synergy (DS) for glucose and xylose could reach 2.57 and 1.84. Moreover, the hydrolysis rate increased evidently, and the reaction time to reach the same yield of glucose and xylose was shortened by 72 h and 96 h, respectively. This study on synergistic mechanisms demonstrated that the addition of Xyn10A could cause the destruction of substrates’ morphology and the dissolution of lignin components but could not change the accessibility and crystallinity of substrate cellulose. The joint effect of cellulase and xylanase during the hydrolysis process was thought to result in a synergistic mechanism.

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Improved Release of Monosaccharides and Ferulic Acid Using Enzyme Blends From Aspergillus Niger and Eupenicillium Parvum

Cited by 3 publications

References 42 publications

Fermenting Distiller’s Grains by the Domesticated Microbial Consortium To Release Ferulic Acid

Fermenting Distiller’s Grains by the Domesticated Microbial Consortium To Release Ferulic Acid

Optimization of Compound Ratio of Exogenous Xylanase and Debranching Enzymes Supplemented in Corn-Based Broiler Diets Using In Vitro Simulated Gastrointestinal Digestion and Response Surface Methodology

Synergistically Enhanced Enzymatic Hydrolysis of Sugarcane Bagasse Mediated by a Recombinant Endo-Xylanase from Streptomyces ipomoeae

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