Improved removal of viruslike particles from purified monoclonal antibody IgM preparation via virus filtration

Maerz, Holger K.; Hahn, S.; Maaßen, Anke; Meisel, Helga; Roggenbuck, Dirk; Sato, Tetsuo; Tanzmann, H.; Emmrich, Frank; Marx, Uwe

doi:10.1038/nbt0596-651

Cited by 8 publications

(2 citation statements)

References 14 publications

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“…Realistically, viruses the size of polio-or parvoviruses (20-25 nm) cannot be separated from similarly sized proteins by such filters, since in this size range larger proteins are already held back. Yet Maerz et al 1 Success in solving this separation problem may be attributed to the different static properties of viruses and antibodies. Antibodies are very flexible and can slip through the narrow membrane pores at slow flow rates, whereas small, nonenveloped viruses must be considered to be rigid molecules that are restrained by the membrane.…”

Section: Viral Clearancementioning

confidence: 99%

Avoiding viral contamination in biotechnological and pharmaceutical processes

Henzler

Kaiser

1998

Nat Biotechnol

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Section: Viral Clearancementioning

confidence: 99%

Avoiding viral contamination in biotechnological and pharmaceutical processes

Henzler

Kaiser

1998

Nat Biotechnol

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“…Recently, large pore ultrafiltration membranes with MWCO (150$800 kDa) have been developed as virus filtration membranes (Christy and Vermant, 2002;Hamamoto et al, 1989;Maerz et al, 1996). Here the aim is to validate virus clearance by greatly reducing the number of contaminating viruses by retaining virions that may be present in the product stream.…”

Section: Introductionmentioning

confidence: 99%

Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification

Wickramasinghe

Kalbfuss

Zimmermann

et al. 2005

Biotechnol. Bioeng.

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Large scale purification of viruses and viral vectors for gene therapy applications and viral vaccines is a major separation challenge. Here tangential flow microfiltration and ultrafiltration using flat sheet membranes has been investigated for concentration of human influenza A virus. Ultrafiltration membranes with molecular weight cutoffs of 100 and 300 kDa as well as 0.1, 0.2 and 0.45 microm microfiltration membranes have been tested. The results indicate that use of 300 kDa membranes not only concentrate the virus particles but also lead to a significant removal of host cell proteins and DNA in the permeate. Tangential flow filtration may be used to fractionate virus particles. Human influenza A virus particles are spherical with an average size of 100 nm. Use of a 0.1 microm membrane leads to passage of virus particles less than 100 nm into the permeate and an increase of larger particles in the retentate. These results suggest that control of the transmembrane pressure, membrane pore size and pore size distribution could enable isolation of intact virus particles from damaged virions. Isolation of the virus particles of interest from viral fragments and other particulate matter could result in simplification of subsequent purification steps. Larger pore size membranes such as 0.45 microm that allow the passage of all virus particles may be used to remove host cell fragments. In addition virus particles attached to these fragments will be removed. Careful selection of membrane morphology and operating conditions will be essential in order to maximize the benefit of tangential flow filtration steps in the purification of viral products from cell cultures.

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Antibody Purification

Ultee¹,

Rea²

1999

Encyclopedia of Bioprocess Technology

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Improved removal of viruslike particles from purified monoclonal antibody IgM preparation via virus filtration

Cited by 8 publications

References 14 publications

Avoiding viral contamination in biotechnological and pharmaceutical processes

Avoiding viral contamination in biotechnological and pharmaceutical processes

Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification

Antibody Purification

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