Improved resolution of the human adipose tissue proteome at alkaline and wide range pH by the addition of hydroxyethyl disulfide

Cortón, Marta; Villuendas, Gemma; Botella, J. I.; Millán, José L. San; Escobar-Morreale, Héctor F.; Peral, Belén

doi:10.1002/pmic.200300644

Cited by 54 publications

(45 citation statements)

References 17 publications

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“…CT-1 was first described in cardiac muscle cells, but different levels of CT-1 expression have also been found in a variety of tissues outside the cardiac compartment (liver, lung, kidney, skeletal muscle, pancreas, and others) (32). Adipose tissue is not only a storage tissue, but it is also an active endocrine organ that secretes numerous proinflammatory hormones and cytokines, such as TNF-␣ and IL-6 (21), and multiple proteomic tools have been used to characterize the secretome of preadipocyte cell lines, primary adipocytes, and adipose tissue (1,8,23). However, no information is available about whether adipose tissue expresses the cytokine CT-1.…”

Section: Discussionmentioning

confidence: 99%

Cardiotrophin-1 is expressed in adipose tissue and upregulated in the metabolic syndrome

Natal

Fortuño²,

Restituto³

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

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Section: Discussionmentioning

confidence: 99%

Cardiotrophin-1 is expressed in adipose tissue and upregulated in the metabolic syndrome

Natal

Fortuño²,

Restituto³

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

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“…Samples were crushed with a disposable pestle and sonicated in icewater four times (10 pulses of 5 s each time) at intervals of 15 min with gentle shaking at room temperature between intervals. Samples were then centrifuged at 80,000 ϫ g for 1.5 h at 19°C (23). The central aqueous layer between the top lipid layer and the cell debris pellet was recovered, residual lipids were removed with a second centrifugation at 25,000 ϫ g for 30 min at 4°C, and the aliquots were stored at Ϫ80°C.…”

Section: Methodsmentioning

confidence: 99%

Amyloidogenic and Associated Proteins in Systemic Amyloidosis Proteome of Adipose Tissue

Lavatelli

Perlman

Spencer

et al. 2008

Molecular & Cellular Proteomics

140

126

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In systemic amyloidoses, widespread deposition of protein as amyloid causes severe organ dysfunction. It is necessary to discriminate among the different forms of amyloid to design an appropriate therapeutic strategy. We developed a proteomics methodology utilizing two-dimensional polyacrylamide gel electrophoresis followed by matrix-assisted laser desorption/ionization mass spectrometry and peptide mass fingerprinting to directly characterize amyloid deposits in abdominal subcutaneous fat obtained by fine needle aspiration from patients diagnosed as having amyloidoses typed as immunoglobulin light chain or transthyretin. Striking differences in the two-dimensional gel proteomes of adipose tissue were observed between controls and patients and between the two types of patients with distinct, additional spots present in the patient specimens that could be assigned as the amyloidogenic proteins in full-length and truncated forms. In patients heterozygotic for transthyretin mutations, wild-type peptides and peptides containing amyloidogenic transthyretin variants were isolated in roughly equal amounts from the same protein spots, indicative of incorporation of both species into the deposits. Furthermore novel spots unrelated to the amyloidogenic proteins appeared in patient samples; some of these were identified as isoforms of serum amyloid P and apolipoprotein E, proteins that have been described previously to be associated with amyloid deposits. Finally changes in the normal expression pattern of resident adipose proteins, such as down-regulation of ␣B-crystallin, peroxiredoxin 6, and aldo-keto reductase I, were observed in apparent association with the presence of amyloid, although their levels did not strictly correlate with the grade of amyloid deposition. This proteomics approach not only provides a way to detect and unambiguously type the deposits in abdominal subcutaneous fat aspirates from patients with amyloidoses but it may also have the capability to generate new insights into the mechanism of the diseases by identifying novel proteins or protein post-translational modifications associated with amyloid infiltration. Molecular & Cellular Proteomics 7:1570 -1583, 2008.

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“…To date, however, only two studies have investigated the proteome of human and mouse adipose tissue; one resolved about 100 human proteins using wide IPG strips and identified 16 polypeptides by means of mass spectrometry (39), while the other resolved a considerable number of murine white adipose tissue proteins and identified 80 unique polypeptides (40).…”

mentioning

confidence: 99%

Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients

Celis

Moreira

Cabezón

et al. 2005

Molecular & Cellular Proteomics

208

164

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It has become clear that growth and progression of breast tumor cells not only depend on their malignant potential but also on factors present in the tumor microenvironment. Of the cell types that constitute the mammary stroma, the adipocytes are perhaps the least well studied despite the fact that they represent one of the most prominent cell types surrounding the breast tumor cells. There is compelling evidence demonstrating a role for the mammary fat pad in mammary gland development, and some studies have revealed the ability of fat tissue to augment the growth and ability to metastasize of mammary carcinoma cells. Very little is known, however, about which factors adipocytes produce that may orchestrate these actions and how this may come about. In an effort to shed some light on these questions, we present here a detailed proteomic analysis, using two-dimensional gel-based technology, mass spectrometry, immunoblotting, and antibody arrays, of adipose cells and interstitial fluid of fresh fat tissue samples collected from sites topologically distant from the tumors of high risk breast cancer patients that underwent mastectomy and that were not treated prior to surgery. A total of 359 unique proteins were identified, including numerous signaling molecules, hormones, cytokines, and growth factors, involved in a variety of biological processes such as signal transduction and cell communication; energy metabolism; protein metabolism; cell growth and/or maintenance; immune response; transport; regulation of nucleobase, nucleoside, and nucleic acid metabolism; and apoptosis. Apart from providing a comprehensive overview of the mammary fat proteome and its interstitial fluid, the results offer some insight as to the role of adipocytes in the breast tumor microenvironment and provide a first glance of their molecular cellular circuitry. In addition, the results open new possibilities to the study of obesity, which has a strong association with type 2 diabetes, hypertension, and coronary heart disease. Molecular & Cellular Proteomics 4: 492-522, 2005.During the last years there have been numerous reports indicating that growth and progression of breast as well as other tumor cells depend not only on their malignant potential but also on stromal factors present in the tumor microenvironment, the insoluble extracellular matrix as well as cell-cell interactions (Refs.

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Improved resolution of the human adipose tissue proteome at alkaline and wide range pH by the addition of hydroxyethyl disulfide

Cited by 54 publications

References 17 publications

Cardiotrophin-1 is expressed in adipose tissue and upregulated in the metabolic syndrome

Cardiotrophin-1 is expressed in adipose tissue and upregulated in the metabolic syndrome

Amyloidogenic and Associated Proteins in Systemic Amyloidosis Proteome of Adipose Tissue

Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients

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