Improved risk assessment of coronary artery disease by substituting paraoxonase 1 activity for HDL-C: Novel cardiometabolic biomarkers based on HDL functionality

Mahrooz, Abdolkarim; Shokri, Yasaman; Variji, Atena; Zargari, Mehryar; Alizadeh, Ahad; Mehtarian, Ehsan

doi:10.1016/j.numecd.2020.12.026

Cited by 11 publications

(6 citation statements)

References 47 publications

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“…17 Como a DAC é uma doença importante em relação à mortalidade e os estudos mostram que existe uma associação entre PON1 e DAC, decidiu-se investigar essa associação na sociedade iraniana, principalmente no nordeste do país. 18 Existem poucos estudos sobre a associação entre o genótipo ou fenótipo de PON1 e DAC no nordeste do Irã, [19][20][21] e a atividade enzimática sérica não foi estudada juntamente com polimorfismos. O objetivo do presente estudo foi avaliar a associação entre polimorfismos de PON1 e atividade da paraoxonase com DAC em adultos iranianos que vivem no nordeste do país.…”

Section: Introductionunclassified

Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

Soflaei¹,

Baktashian²,

Moghaddam³

et al. 2022

Arquivos Brasileiros De Cardiologia

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Fundamento: Tem sido demonstrado que um aumento dos níveis séricos de PON1 é protetor contra vários distúrbios. Foi relatado que vários polimorfismos de nucleotídeo único (SNPs, single nucleotide polymorphisms) do gene PON1 estão associados a níveis e atividade de proteínas enzimáticas séricas. Objetivos: Investigar a associação de SNPs do PON1 e atividade da paraoxonase sérica com a doença arterial coronariana (DAC). Métodos: Foram estudados 601 pacientes não relacionados submetidos à angiografia coronária, incluindo aqueles com estenose >50% (N=266) e aqueles com estenose <30% (N=335). Os SNPs rs662 e rs840560 do gene da paraoxonase foram determinados utilizando o método ARMS-PCR e o SNP rs705379 foi genotipado utilizando análise de PCR-RFLP. A atividade da paraoxonase sérica foi medida utilizando paraoxon como substrato. O valor de p<0,05 foi considerado significante. Resultados: A atividade da paraoxonase sérica não foi significativamente diferente entre os grupos de estudo. Após ajuste para idade, sexo, hipertensão, diabetes mellitus e dislipidemia, o genótipo GG e o modelo codominante de rs662 foram positivamente associados a uma angiografia positiva (respectivamente, OR = 2,424, IC 95% [

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Section: Introductionunclassified

Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

Soflaei¹,

Baktashian²,

Moghaddam³

et al. 2022

Arquivos Brasileiros De Cardiologia

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“…Paraoxonase 1 (PON1), a component of HDL particles, reduces lipid peroxidation of other lipoproteins, and the antioxidant capacity of HDL depends mainly on its content [ 5 ]. Measuring lipid peroxidation products, namely conjugated dienes, is another approach to assessing HDL antioxidant capacity and therefore the lipoprotein’s function [ 6 ]. Furthermore, studies have shown that HDL lipid composition (phospholipids, triglycerides, esterified, and free cholesterol) and transfer are predictors of premature CAD [ 4 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Overall antioxidant defense influences HDL function [ 6 ]. One pivotal protein in antioxidant defense is sirtuin-1 (Sirt-1), which has been associated with better cardiometabolic risk factors control and longevity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

The Interplay of Sirtuin-1, LDL-Cholesterol, and HDL Function: A Randomized Controlled Trial Comparing the Effects of Energy Restriction and Atorvastatin on Women with Premature Coronary Artery Disease

et al. 2022

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Introduction: HDL function has gained prominence in the literature as there is a greater predictive capacity for risk in early coronary artery disease when compared to the traditional parameters. However, it is unclear how dietary energy restriction and atorvastatin influence HDL function. Methods: A randomized controlled trial with 39 women with early CAD divided into three groups (n = 13): energy restriction (30% of VET), atorvastatin (80 mg), and control. Analyses of traditional biochemical markers (lipid and glucose profile), circulating Sirt-1, and HDL function (lipid composition, lipid transfer, and antioxidant capacity). Results: Participants’ mean age was 50.5 ± 3.8 years. Energy restriction increased Sirt-1 by 63.6 pg/mL (95%CI: 1.5–125.7; p = 0.045) and reduced BMI by 0.8 kg/m2 (95%CI: −1.349–−0.273; p = 0.004) in a manner independent of other cardiometabolic factors. Atorvastatin reduced LDL-c by 40.0 mg/dL (95%CI: −69.910–−10.1; p = 0.010). Increased Sirt-1 and reduced BMI were independently associated with reduced phospholipid composition of HDL (respectively, β = −0.071; CI95%:−0.136–−0.006; p = 0.033; β = 7.486; CI95%:0.350–14.622; p = 0.040). Reduction in BMI was associated with lower HDL-free cholesterol (β = 0.818; CI95%:0.044–1.593; p = 0.039). LDL-c reduction by statins was associated with reduced maximal lipid peroxide production rate of HDL (β = 0.002; CI95%:0.000–0.003; p = 0.022) and total conjugated diene generation (β = 0.001; CI95%:0.000–0.001; p = 0.029). Conclusion: This study showed that energy restriction and atorvastatin administration were associated with changes in lipid profile, serum Sirt-1 concentrations, and HDL function.

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“…Moreover, in physiological conditions, HDLs have been recently reported to exert other functions, including anti-inflammatory and antioxidant capacity, highly dependent on HDL composition [ 2 ]. The antioxidant capacity of HDLs is mainly conferred by the presence of apolipoproteins and enzymes transported by HDLs, including paraoxonase 1 (PON1) [ 3 ]. In addition, lipid components are essential in HDL-related antioxidant capacity in order to prevent their own oxidation and low-density lipoproteins (LDLs) oxidation [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells

Grao‐Cruces

Millán-Linares²,

Martin-Rubio

et al. 2021

Biomedicines

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High-density lipoproteins (HDLs) play an important role in reverse cholesterol transport and present antioxidant properties, among others. In the central nervous system (CNS), there are HDLs, where these lipoproteins could influence brain health. Owing to the new evidence of HDL functionality remodeling in obese patients, and the fact that obesity-associated metabolic disturbances is pro-inflammatory and pro-oxidant, the aim of this study was to investigate if HDL functions are depleted in obese patients and obesity-associated microenvironment. HDLs were isolated from normal-weight healthy (nwHDL) and obese men (obHDL). The oxHDL level was measured by malondialdehyde and 4-hydroxynoneal peroxided products. BV2 microglial cells were exposed to different concentrations of nwHDL and obHDL in different obesity-associated pro-inflammatory microenvironments. Our results showed that hyperleptinemia increased oxHDL levels. In addition, nwHDLs reduced pro-inflammatory cytokines’ release and M1 marker gene expression in BV2 microglial cells. Nevertheless, both nwHDL co-administered with LPS+leptin and obHDL promoted BV2 microglial activation and a higher pro-inflammatory cytokine production, thus confirming that obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of HDLs in microglial cells.

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Improved risk assessment of coronary artery disease by substituting paraoxonase 1 activity for HDL-C: Novel cardiometabolic biomarkers based on HDL functionality

Cited by 11 publications

References 47 publications

Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

Associação do Genótipo e Fenótipo da Paraoxonase-1 com Angiografia Positiva para Doença Arterial Coronariana

The Interplay of Sirtuin-1, LDL-Cholesterol, and HDL Function: A Randomized Controlled Trial Comparing the Effects of Energy Restriction and Atorvastatin on Women with Premature Coronary Artery Disease

Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells

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