Improved sample preparation method for selected persistent organochlorine pollutants in human serum using solid-phase disk extraction with gas chromatographic analysis

Pauwels, An; Wells, David A.; Covaci, Adrian; Schepens, Paul

doi:10.1016/s0378-4347(98)00493-9

Cited by 61 publications

(41 citation statements)

References 20 publications

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“…The sample preparation was set up by adapting a method established by the Laboratory of Food Analysis (University of Liège, Belgium) according to Singh et al (1998), Janak et al (1999, Pauwels et al (1999) and Frenich et al (2000). Serum was first deproteinised by adding 100 µl of triethylamine and 10 ml of formic acid to a precisely known volume of sample (from 2.5 to 10 ml, depending on the amount of sample available).…”

Section: Methodsmentioning

confidence: 99%

Quantitative dynamics of PCB transfer from mother to pup during lactation in UK grey seals Halichoerus grypus

Debier¹,

Pomeroy²,

Dupont³

et al. 2003

Mar. Ecol. Prog. Ser.

136

101

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Section: Methodsmentioning

confidence: 99%

Quantitative dynamics of PCB transfer from mother to pup during lactation in UK grey seals Halichoerus grypus

Debier¹,

Pomeroy²,

Dupont³

et al. 2003

Mar. Ecol. Prog. Ser.

136

101

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“…Complete details of the solid-phase disk extraction are described and evaluated elsewhere (19). The method was shown to be consistent at three fortification levels.…”

Section: Methodsmentioning

confidence: 99%

Comparison of chemical-activated luciferase gene expression bioassay and gas chromatography for PCB determination in human serum and follicular fluid.

Pauwels

Cenijn

Schepens

et al. 2000

Environ Health Perspect

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We assessed exposure to dioxin-like compounds using chemical and bioassay analysis in different matrices in a female population. A total of 106 serum and 9 follicular fluid samples were collected from infertile women attending Centers for Reproductive Medicine in Belgium from 1996 to 1998. Major polychlorinated biphenyl (PCB) congeners were quantified by chemical analysis using gas chromatography with electron-capture detection, and the chemical-activated luciferase gene expression (CALUX) bioassay was used to determine the total dioxin-like toxic equivalence (TEQ) of mixtures of polyhalogenated aromatic hydrocarbons present in body fluids, such as serum and follicular fluid. To the best of our knowledge, this is the first investigation to determine TEQ values by the CALUX bioassay in follicular fluid. The TEQ levels in both matrices are well correlated (r = 0.83, p = 0.02). As the chemical and bioassay analysis executed in this study do not cover the same span of polyhalogenated aromatic hydrocarbons, we did not expect totally correlated results. Moreover, the sample workup and quantification of the analytes differed completely. Nonetheless, the TEQ values in human extracts correlated well with the sum of four major PCB congeners chemically determined in both serum and follicular fluid. These results indicate that the CALUX bioassay may serve as a simple, relatively inexpensive prescreening tool for exposure assessment in epidemiologic surveys. Images Figure 1 Figure 2

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“…The mixture was homogenized by vortexing for 1 min. Blood samples were prepared by a method modified from Pauwels et al (1999), Janak et al (1999) and Sunbdberg et al (2006). Four millilitres of blood were first deproteinised by adding 100 lL of triethylamine and 5 mL of formic acid; 50 lL of PCB 112 (100 pg lL À1 in acetone) were used as surrogate marker.…”

Section: Sample Preparationmentioning

confidence: 99%

Maternal transfer of chlorinated contaminants in the leatherback turtles, Dermochelys coriacea, nesting in French Guiana

et al. 2010

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a b s t r a c tWe examined the maternal transfer of organochlorine contaminants (OCs), pesticides (DDTS and HCHs) and polychlorinated biphenyls (PCBs), and the temporal variation of blood and eggs concentrations from 38 leatherback turtles (Dermochelys coriacea) nesting in French Guiana. PCBs were found to be the dominant OCs with respective mean concentrations of 55.14 ng g À1 lipid-mass for egg and 1.26 ng mL À1 wetmass for blood. OC concentrations were lower than concentrations measured in other marine turtles which might be due to the lower trophic position (diet based on gelatinous zooplankton) and to the location of their foraging and nesting grounds. All OCs detected in leatherback blood were detected in eggs, suggesting a maternal transfer of OCs. This transfer was shown to depend on female blood concentration for RDDTs and for the most prevalent PCB congeners, since significant relationships were found between paired blood-egg concentrations. During the nesting season, OC concentrations in eggs and the percentage of lipid in eggs were found to decline in successive clutches, highlighting a process of offloading from females to their eggs and a decreasing investment of lipid from females into their clutches. OCs in eggs tended to be higher in females spending 3 years in the foraging grounds between two nesting seasons than in those spending 2 years, suggesting an impact of time spacing two breeding seasons, called remigration interval, and of location of the foraging grounds.

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Improved sample preparation method for selected persistent organochlorine pollutants in human serum using solid-phase disk extraction with gas chromatographic analysis

Cited by 61 publications

References 20 publications

Quantitative dynamics of PCB transfer from mother to pup during lactation in UK grey seals Halichoerus grypus

Quantitative dynamics of PCB transfer from mother to pup during lactation in UK grey seals Halichoerus grypus

Comparison of chemical-activated luciferase gene expression bioassay and gas chromatography for PCB determination in human serum and follicular fluid.

Maternal transfer of chlorinated contaminants in the leatherback turtles, Dermochelys coriacea, nesting in French Guiana

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