A detailed discussion is given of the analysis of recent data to obtain improved upper bounds on the couplings |Ue4| 2 and |Uµ4| 2 for a mainly sterile neutrino mass eigenstate ν4. Using the excellent agreement among Ft values for superallowed nuclear beta decay, an improved upper limit is derived for emission of a ν4. The agreement of the ratios of branching ratios R (π) e/µ = BR(π + → e + νe)/BR(π + → µ + νµ), R (K) e/µ , R (Ds) e/τ , R (Ds) µ/τ , and R (D)e/τ , and the branching ratios BR(B + → e + νe) and BR(B + → µ + νµ) decays with predictions of the Standard Model, is utilized to derive new constraints on ν4 emission covering the ν4 mass range from MeV to GeV. We also discuss constraints from peak search experiments probing for emission of a ν4 via lepton mixing, as well as constraints from pion beta decay, CKM unitarity, µ decay, leptonic τ decay, and other experimental inputs.