2021
DOI: 10.1016/j.jinf.2020.05.061
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Improved sensitivity using a dual target, E and RdRp assay for the diagnosis of SARS-CoV-2 infection: Experience at a large NHS Foundation Trust in the UK

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Cited by 32 publications
(37 citation statements)
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“…following treatment with Prolectin-M, Ct value of Rd/Rp + N gene increased by16.41 points, 95% (CI -0.3527 to 32.48, p=0.047). Similarly, small envelope (E) gene also increased by 17.75 points (95% CI;-0.1321 to 35.63, p = 0.05). The expression of N1, N2 genes went below detectable thresholds by day 7, (Mann whitney U = 0.000, p<0.029).…”
Section: Resultsmentioning
confidence: 92%
“…following treatment with Prolectin-M, Ct value of Rd/Rp + N gene increased by16.41 points, 95% (CI -0.3527 to 32.48, p=0.047). Similarly, small envelope (E) gene also increased by 17.75 points (95% CI;-0.1321 to 35.63, p = 0.05). The expression of N1, N2 genes went below detectable thresholds by day 7, (Mann whitney U = 0.000, p<0.029).…”
Section: Resultsmentioning
confidence: 92%
“…Surprisingly, one target in the N gene (n3) was positive in all positive cases, whereas the rdrp1 and rdrp2 targets did less well, consistent with previous reports. 26 The design of the cartridge, with each assay distributed across the analytical unit, means that this difference is more likely due to biological differences in assay performance, than technical performance of the cartridge. Future adaptations will be to replace redundant assays with targets for respiratory syndromic screening (eg, influenza or respiratory syncytial virus) in anticipation of the diagnostic challenges on entering annual influenza season.…”
Section: Resultsmentioning
confidence: 99%
“…In order to optimize the performance of a SARS-Cov-2 detection test and in particular its sensitivity, the concomitant detection of several genes is often to be preferred. Even if detection of the E gene alone is not recommended by the European Center for Disease Prevention and Control (ECDC) because of specificity problems and its vulnerability to sample contamination [75], it is nevertheless recommended to use the E gene as a target, along with the RdRP gene for confirmation [54]. Detection of the E gene can also be combined with detection of the N gene.…”
Section: Molecular Targets Selectedmentioning
confidence: 99%