Improved separation of bovine serum albumin and lactoferrin mixtures using charged ultrafiltration membranes

Valiño, Virginia; Román, M. Fresnedo San; Ibáñez, Raquel; Ortiz, Inmaculada

doi:10.1016/j.seppur.2014.01.023

Cited by 48 publications

(51 citation statements)

References 46 publications

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“…The values obtained for the BSA rejection and water flux recommend the use of these membranes for ultrafiltration processes with potential applications in proteins retention, colloidal aggregates, saccharides and polysaccharides, or macromolecular compounds. The CA/GO composite membranes showed superior behavior for the rejection of BSA in comparison with other CA membranes [36] or even other composite CA membranes, [37] the average retention being around 75-80%. Another potential explanation in the case of our composite membranes could be the weak interaction between GO surface in the membrane pores and first protein molecules which penetrate the active surface of the membrane (interaction between delocalized electrons at the surface of graphene and non-participants electrons from protein functional groups).…”

Section: Fluxesmentioning

confidence: 89%

Fabrication of cellulose triacetate/graphene oxide porous membrane

Ioniță

Crică

Voicu

et al. 2015

Polym. Adv. Technol.

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Cellulose triacetate (AC)/graphene oxide (GO) porous membranes were successfully fabricated by combining ultrasonication and phase inversion method. The structures and morphologies of the resultant composite membranes were investigated by X-ray diffraction (XRD), scanning electron microscopy, and transmission electron microscopy, respectively. Microscopic and X-ray diffraction measurements revealed that GO sheets were uniformly dispersed within the AC matrix. The pore size and structure were modulated by changing GO concentration from 0.25 to 1 wt%. Membrane thermal properties were also studied. Among all tested membranes, the most favorable GO amount was 1 wt%, giving Td 3% of 274°C, which represents a 22°C enhancement compared with AC. Conversely, the membranes showed improved barrier properties against water and ethanol. The decrease of both ethanol and water fluxes was assigned to the stabilization of composite membrane structure, as a result of GO progressive addition. Bovine serum albumin rejection assay indicated an increasing from 78% in the case of CA membrane to 99% in the case of CA/GO 1 wt% of the rejection degree after 90 min.

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Section: Fluxesmentioning

confidence: 89%

Fabrication of cellulose triacetate/graphene oxide porous membrane

Ioniță

Crică

Voicu

et al. 2015

Polym. Adv. Technol.

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“…Taking into account the results previously obtained, Figure 9 collects the summary of the best experimental conditions to improve the efficiency of BSA/LF proteins separation using charged ultrafiltration membranes [3]. …”

Section: Selection Of the Most Suitable Experimental Conditionsmentioning

confidence: 99%

“…Whey is a high and varied mixture of secreted proteins, which contain a wide range of chemical, physical, and functional properties [2]. Among them, bovine serum albumin (BSA) has foaming and gelling properties and bovine lactoferrin (LF) presents important nutraceutical and anti-inflammatory or antimicrobial properties and plays a significant role in iron metabolism [3]. Such properties made its use interesting in food and pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Valiño

Román

Ibáñez

et al. 2014

Separation and Purification Technology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. KeywordsDynamic light scattering, Zeta Potential, Bovine Serum Proteins, Bioprocess Design. AbstractThe aim of this work is to accurately measure fundamental surface properties, i.e., zeta potential, isoelectric point and protein size that determine the optimal separation conditions of Bovine serum albumin and lactoferrin, two high added value food proteins whose similarity in weight makes their separation a scientific and technical challenge.

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“…Although many techniques have been developed to isolate LF, the separation of high-value minor proteins of similar molecular weights, such as BSA and LF, remains a challenge [25,26]. BSA is a 66.5-kDa protein with an isoelectric point near 4.7; LF is a 78.0-kDa protein with an isoelectric point around 8.7.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Membrane Chromatography and Monolith Chromatography for Lactoferrin and Bovine Serum Albumin Separation

2016

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These last few decades, membranes and monoliths have been increasingly used as stationary phases for chromatography. Their fast mass transfer is mainly based on convection, which leads to reduced diffusion, which is usually observed in resins. Nevertheless, poor flow distribution, which causes inefficient binding, remains a major challenge for the development of both membrane and monolith devices. Moreover, the comparison of membranes and monoliths for biomolecule separation has been very poorly investigated. In this paper, the separation of two proteins, bovine serum albumin (BSA) and lactoferrin (LF), with similar sizes, but different isoelectric points, was investigated at a pH of 6.0 with a BSA-LF concentration ratio of 2/1 (2.00 mg·mL −1 BSA and 1.00 mg·mL −1 LF solution) using strong cation exchange membranes and monoliths packed in the same housing, as well as commercialized devices. The feeding flow rate was operated at 12.0 bed volume (BV)/min for all devices. Afterward, bound LF was eluted using a phosphate-buffered saline solution with 2.00 M NaCl. Using membranes in a CIM housing from BIA Separations (Slovenia) with porous frits before and after the membrane bed, higher binding capacities, sharper breakthrough curves, as well as sharper and more symmetric elution peaks were obtained. The monolith and commercialized membrane devices showed lower LF binding capacity and broadened and non-symmetric elution peaks.

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Improved separation of bovine serum albumin and lactoferrin mixtures using charged ultrafiltration membranes

Cited by 48 publications

References 46 publications

Fabrication of cellulose triacetate/graphene oxide porous membrane

Fabrication of cellulose triacetate/graphene oxide porous membrane

Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Comparison of Membrane Chromatography and Monolith Chromatography for Lactoferrin and Bovine Serum Albumin Separation

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