Improved sister-chromatid differentiation using paraffin-coated bromodeoxyuridine tablets in mice

McFee, A.F.; Lowe, Karen W.; Sebastián, Juan

doi:10.1016/0165-7992(83)90042-8

Cited by 88 publications

(6 citation statements)

References 6 publications

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“…Paraffin-coated BrdU tablets (50 mg) following the methodology of McFee et al (1983) and Sharief et al (1986) were used for in vivo SCE studies and cell replication kinetics analyses. Approximately 80% of the surface of the tablets was coated with embedding paraffin and implanted subcutaneously on the flank of mice under ether anesthesia.…”

Section: In Vivo Sister-chromatid Exchange Assaymentioning

confidence: 99%

Sister-chromatid exchange and chromosome aberrations for 4 aliphatic epoxides in mice

Giri

Messerly

Sinsheimer

1989

Mutation Research/Genetic Toxicology

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Sister-chromatid exchange (SCE) and chromosome aberrations (CA) in bone marrow cells were analyzed after in vivo exposure in mice to 4 aliphatic epoxides, namely 1-naphthyl glycidyl ether (NGE), 1-naphthyl propylene oxide (NPO), 4-nitrophenyl glycidyl ether (NPGE) and trichloropropylene oxide (TCPO). These compounds were selected as being among the most mutagenic aliphatic epoxides in our previous structure-mutagenicity studies with the Ames test. There were significant dose-related increases in SCE and CA results for all 4 epoxides. The order of genotoxicity as established through SCE was NGE greater than NPO greater than NPGE approximately equal to TCPO greater than solvent control. It is of interest that Ames Salmonella results are consistent with in vivo genotoxicity for these compounds. However, only the plate test version of the Ames procedure is consistent with this order of in vivo genotoxicity and neither preincubation Ames testing results nor chemical alkylation rates would have predicted this order.

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Section: In Vivo Sister-chromatid Exchange Assaymentioning

confidence: 99%

Sister-chromatid exchange and chromosome aberrations for 4 aliphatic epoxides in mice

Giri

Messerly

Sinsheimer

1989

Mutation Research/Genetic Toxicology

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“…Paraffin-coated 5-bromodeoxyuridine tablets (50 mg), following the methodology of McFee et al [1983] and Sharief et al [1986] were used for in vivo SCE studies and cell replication kinetics analyses. Approximately 80% of the surface of the tablets was coated with embedding paraffin and the tablets were implanted subcutaneously on the flank of mice under ether anesthesia.…”

Section: Sce Assaymentioning

confidence: 99%

Mutagenicity in salmonella and sister chromatid exchange in mice for 1,4‐, 1,3‐, 2,4‐, and 3,4‐dimethylphenanthrenes

Sinsheimer

Giri

Hooberman

et al. 1991

Environ and Mol Mutagen

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The mutagenicity in Salmonella and in vivo sister chromatid exchange in the bone-marrow cells of mice was determined for 1,4-, 1,3-, 2,4-, and 3,4-dimethylphenanthrene (DMPh) with the objective to study the relative importance of substitution at the 1 and 4 positions of this series of methylated phenanthrenes. For both tests, 1,4- DMPh was decidedly more genotoxic than the remaining regioisomers. While the well recognized role of steric crowding in the bay region is a factor in this enhanced genotoxicity, equally important is substitution at the 1 position with its potential to inhibit detoxication through 9,10-diol formation.

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“…SCE frequencies and cell replication kinetics were analyzed in mouse bone marrow cells after DNA labeling with BrdUrd. In vivo labelling was achieved with BrdUrd tablet methodology (Allen et al, 1978;McFee et al, 1983). Fifty-milligram BrdUrd tablets were prepared with a Parr Pellet Press and 0.178-in.…”

Section: In Vivo Sister Chromatid Exchange Analysis In Micementioning

confidence: 99%

Mutagenic screening of marker grenade dyes by the salmonella reversion assay, l5178y/tk^± mouse lymphoma assay, and in vivo sister chromatid exchange analysis in mice

et al. 1988

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Two dyes (C.I. Solvent Yellow No. 33 and a mixture of C.I. Solvent Yellow No. 33 and C.I. Solvent Green No. 3) were tested for mutagenicity in the Salmonella reversion assay and the L5178Y/TK± mouse lymphoma assay, and also for sister chromatid exchange (SCE) induction in vivo in C57B1/6J mice. In addition, a > 99.9% pure sample of the yellow dye [2‐(2′‐quinolyl)‐1,3‐indandione] was tested with and without exogenous activation in the Salmonella reversion assay and the L5178Y/TK± mouse lymphoma assay. Neither C.I. Solvent Yellow No. 33 nor the C.I. Solvent Yellow No. 33 and Solvent Green No. 3 mixture was positive for inducing SCEs in vivo. All three dyes were tested in the standard plate incorporation test in seven Salmonella strains TA98, TA100, TA102, TA104, TA1535, TA1537, and TA1538. The dyes were negative with and without exogenous activation in TA98, TA1535, and TA1538. One test with TA1537 was positive with the > 99.9% purified yellow dye. All three dyes gave weakly positive results (less than a twofold increase) with S‐9 in TA100 and were clearly positive in TA102 and TA104 both with and without S‐9. They also induced mutation at the thymidine kinase locus in mouse.

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Improved sister-chromatid differentiation using paraffin-coated bromodeoxyuridine tablets in mice

Cited by 88 publications

References 6 publications

Sister-chromatid exchange and chromosome aberrations for 4 aliphatic epoxides in mice

Sister-chromatid exchange and chromosome aberrations for 4 aliphatic epoxides in mice

Mutagenicity in salmonella and sister chromatid exchange in mice for 1,4‐, 1,3‐, 2,4‐, and 3,4‐dimethylphenanthrenes

Mutagenic screening of marker grenade dyes by the salmonella reversion assay, l5178y/tk^± mouse lymphoma assay, and in vivo sister chromatid exchange analysis in mice

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Improved sister-chromatid differentiation using paraffin-coated bromodeoxyuridine tablets in mice

Cited by 88 publications

References 6 publications

Sister-chromatid exchange and chromosome aberrations for 4 aliphatic epoxides in mice

Sister-chromatid exchange and chromosome aberrations for 4 aliphatic epoxides in mice

Mutagenicity in salmonella and sister chromatid exchange in mice for 1,4‐, 1,3‐, 2,4‐, and 3,4‐dimethylphenanthrenes

Mutagenic screening of marker grenade dyes by the salmonella reversion assay, l5178y/tk± mouse lymphoma assay, and in vivo sister chromatid exchange analysis in mice

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Mutagenic screening of marker grenade dyes by the salmonella reversion assay, l5178y/tk^± mouse lymphoma assay, and in vivo sister chromatid exchange analysis in mice