Improved spectrophotometric method for determining amorphine in the fruits of Amorpha fruticosa

Genkina, G. L.; Shakirov, T. T.; Shamsutdinov, R. I.

doi:10.1007/bf00771414

Cited by 2 publications

(2 citation statements)

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“…The extracts for fruticin assay were prepared essentially as described by Genkina et al (1971). In brief, approximately 1 g of powdered herbal substance was weighed accurately, macerated 24 h with methanol (25 mL) and further shaken for 4 h at the room temperature.…”

Section: 2 Preparation Of Extractsmentioning

confidence: 99%

Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia

et al. 2020

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Fruticin (amorphin) is a constituent of the fruit of the false indigo-bush (Amorpha fruticosa L.), which belongs to the class of rotenoid glycosides, and shows several interesting pharmacological activities. The aim of this study was to isolate and chemically characterize this natural product, as well as to determine the optimal period of the year for A. fruticosa fruits collection. Fruticin was obtained by re-crystallization of the precipitate that formed after partial evaporation of the extract, prepared by 3fold extraction of powdered plant material by chloroform - ethanol (1:1, v/v). The structure of the final product was determined by various techniques of instrumental analysis (NMR, UV and MS), and confirmed by comparing the obtained spectra with corresponding data in available literature. The content of fruticin in A. fruticosa fruit was determined by LC-DAD-MS, using the external standard method based on the constructed calibration curve. Limits of detection (LOD) and quantification (LOQ) were also determined. A substantial increase in fruticin content was observed during the ripening period (>50 %). It has also been established that the optimal time for fruit collection is mid-December. Obtained results indicate that the content of fruticin in the A. fruticosa fruit is highly dependable on the time of collection. Since the biosynthesis of secondary plant metabolites is influenced not only by the time of collection, but by numerous other factors as well, additional studies are needed to define, with greater certainty, the conditions that are necessary for design of prospective efficient and sustainable production process.

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Section: 2 Preparation Of Extractsmentioning

confidence: 99%

Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia

et al. 2020

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“…A wealth of phytochemical information about phytochemicals in various plant parts of A. fruticosa is available from scientists from Uzbekistan, who published their results in the 1960s and 1970s of the last century. They report isolation of rotenoid glycosides and aglycones, such as amorphigenin ( 1 ), amorphin ( 21 – arabinoglucoside of 1 ) and their dehydro- and hydroxy- analogs ( Kondratenko et al, 1967 ; Kasymov et al, 1968 , 1969 , 1972 ; Genkina et al, 1971 ; Kadyrova et al, 1973 ; Khodzhaev et al, 1982 ).…”

Section: Phytochemical Constituents Of Amorpha Fruticosa mentioning

confidence: 99%

Amorpha fruticosa – A Noxious Invasive Alien Plant in Europe or a Medicinal Plant against Metabolic Disease?

et al. 2017

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Amorpha fruticosa L. (Fabaceae) is a shrub native to North America which has been cultivated mainly for its ornamental features, honey plant value and protective properties against soil erosion. It is registered amongst the most noxious invasive species in Europe. However, a growing body of scientific literature also points to the therapeutic potential of its chemical constituents. Due to the fact that A. fruticosa is an aggressive invasive species, it can provide an abundant and cheap resource of plant chemical constituents which can be utilized for therapeutic purposes. Additionally, exploitation of the biomass for medicinal use might contribute to relieving the destructive impact of this species on natural habitats. The aim of this review is to provide a comprehensive summary and systematize the state-of-the-art in the knowledge of the phytochemical composition and the potential of A. fruticosa in disease treatment and prevention, with especial emphasis on diabetes and metabolic syndrome. Also reviewed are aspects related to potential toxicity of A. fruticosa which has not yet been systematically evaluated in human subjects.

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Improved spectrophotometric method for determining amorphine in the fruits of Amorpha fruticosa

Cited by 2 publications

References 1 publication

Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia

Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia

Amorpha fruticosa – A Noxious Invasive Alien Plant in Europe or a Medicinal Plant against Metabolic Disease?

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