Improved standardization and potential for shortened time to results with BD Kiestra™ total laboratory automation of early urine cultures: A prospective comparison with manual processing

Graham, Maryza; Tilson, Leanne; Streitberg, Richard; Hamblin, John; Korman, Tony M.

doi:10.1016/j.diagmicrobio.2016.06.020

Cited by 33 publications

(29 citation statements)

References 10 publications

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“…Previous studies suggested earlier growth in microbiological samples when processed with TLA and therefore resulted in shorter TTR [3, 7, 8]. In the present study, the mean time to result for all culture-positive samples was 48.66 h with TLA and 49.98 h pre-TLA.…”

Section: Discussionsupporting

confidence: 55%

“…Significantly more samples showed growth of more than one bacterial species, which has been reported previously [7]. These specimen are classically regarded as contaminated by vaginal or periurethral microbiota [10].…”

Section: Discussionmentioning

confidence: 55%

“…Therefore, there is still potential to decrease the TTR further by longer reading times, earlier, and 24/7 processing of cultures. It has been reported than incubation time may be reduced to 14 h [7] and the time to report may then dramatically decrease. This may then improve the early optimal antibiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

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Significant increase in cultivation of Gardnerella vaginalis, Alloscardovia omnicolens, Actinotignum schaalii, and Actinomyces spp. in urine samples with total laboratory automation

Klein

Nurjadi

Hörner

et al. 2018

Eur J Clin Microbiol Infect Dis

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While total laboratory automation (TLA) is well established in laboratory medicine, only a few microbiological laboratories are using TLA systems. Especially in terms of speed and accuracy, working with TLA is expected to be superior to conventional microbiology. We compared in total 35,564 microbiological urine cultures with and without incubation and processing with BD Kiestra TLA for a 6-month period each retrospectively. Sixteen thousand three hundred thirty-eight urine samples were analyzed in the pre-TLA period and 19,226 with TLA. Sixty-two percent (n = 10,101/16338) of the cultures processed without TLA and 68% (n = 13,102/19226) of the cultures processed with TLA showed growth. There were significantly more samples with two or more species per sample and with low numbers of colony forming units (CFU) after incubation with TLA. Regarding the type of bacteria, there were comparable amounts of Enterobacteriaceae in the samples, slightly less non-fermenting Gram-negative bacteria, but significantly more Gram-positive cocci, and Gram-positive rods. Especially Alloscardivia omnicolens, Gardnerella vaginalis, Actinomyces spp., and Actinotignum schaalii were significantly more abundant in the samples incubated and processed with TLA. The time to report was significantly lower in the TLA processed samples by 1.5 h. We provide the first report in Europe of a large number of urine samples processed with TLA. TLA showed enhanced growth of non-classical and rarely cultured bacteria from urine samples. Our findings suggest that previously underestimated bacteria may be relevant pathogens for urinary tract infections. Further studies are needed to confirm our findings.Electronic supplementary materialThe online version of this article (10.1007/s10096-018-3250-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 55%

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Significant increase in cultivation of Gardnerella vaginalis, Alloscardovia omnicolens, Actinotignum schaalii, and Actinomyces spp. in urine samples with total laboratory automation

Klein

Nurjadi

Hörner

et al. 2018

Eur J Clin Microbiol Infect Dis

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“…Recent studies suggest fully automated microbiology specimen inoculation systems have many advantages, such as improved workflow efficiency and reduced labor costs, operational errors and environmental contamination. [5][6][7][8][9] Previously, Croxatto et al 5 demonstrated that urine specimens processed with the benchtop InoqulA model resulted in better isolated colonies and fewer subcultures. This confirmed the observations made previously by Froment et al 6 with the Kiestra InoqulA and Quiblier et al 10 with urine specimens processed with the Copan WASP system.…”

Section: Discussionmentioning

confidence: 99%

“…Although the BD Kiestra InoqulA system was originally introduced in 2006 and has been evaluated in the United States and Europe, [5][6][7] the system was only recently approved for use in China and this is the first evaluation of the system performance. Additionally, previous studies primarily focused on processing urine specimens and not more challenging specimens where a heavy growth of multiple organisms may be encountered.…”

Section: Introductionmentioning

confidence: 99%

<p>Clinical Performance of BD Kiestra InoqulA Automated System in a Chinese Tertiary Hospital</p>

Yue

Zhou

Zhang

et al. 2020

IDR

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Background: The aim of this study was to evaluate the clinical performance of the BD Kiestra InoqulA automated specimen processing system with commonly encountered clinical microbiology specimens. Materials and Methods: Four types of clinical specimens (sputum, urine, normally sterile body fluids, and feces) were inoculated onto relevant agar plates using a manual method and the BD Kiestra automated system. The number of isolated pathogen species, number of isolated single colonies and uniformity of plate streaking were calculated and compared between two methods. Results: Significantly more isolated colonies were observed on plates inoculated by InoqulA for all specimen types and media with the exception of sputum specimens inoculated onto chocolate agar with vancomycin (P =0.076) and urine onto China blue agar (P =0.856). The quality of plate streaking was also better with InoqulA for all specimen types and media with the exception of urine specimens (P =1.000) and sterile body fluids (P =0.56) inoculated onto China blue agar. Conclusion: This is the first evaluation study of InoqulA with 4 types of clinical specimens in China. It focused on the effect of streaking plates automatically with the magnetic bead. Inoculation of clinical specimens with the BD Kiestra InoqulA system is superior to the manual method for recovery of single colonies and the overall quality of semi-quantitative plate streaking.

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Urine Cultures

Yarbrough

2023

ClinMicroNow

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Urine culture results are reported semiquantitatively, as the colony count is used to guide the microbiological significance of bacteriuria. Thoughtful design and implementation of a urine reflex algorithm may improve diagnostic stewardship by increasing probability of a positive culture and decreasing overutilization of urine culture in cases of asymptomatic bacteriuria. Accordingly, interpretation of bacteria growing from urinary stone culture must take into account organism identification (e.g., urease producer, traditional uropathogen, traditional skin bacteria) and the mechanism by which the stone was obtained, in conjunction with concurrent urine culture results. Urinary stone disease is the result of urinary tract calcification leading to the formation of large mineral deposits. The exact molecular mechanism leading to the formation of urinary stones is unknown; however, there is a well‐understood association between the presence of bacteria within the genitourinary tract and formation of struvite stones.

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Improved standardization and potential for shortened time to results with BD Kiestra™ total laboratory automation of early urine cultures: A prospective comparison with manual processing

Cited by 33 publications

References 10 publications

Significant increase in cultivation of Gardnerella vaginalis, Alloscardovia omnicolens, Actinotignum schaalii, and Actinomyces spp. in urine samples with total laboratory automation

Significant increase in cultivation of Gardnerella vaginalis, Alloscardovia omnicolens, Actinotignum schaalii, and Actinomyces spp. in urine samples with total laboratory automation

<p>Clinical Performance of BD Kiestra InoqulA Automated System in a Chinese Tertiary Hospital</p>

Urine Cultures

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